Literature summary for 3.6.1.23 extracted from

Chan, S.; Segelke, B.; Lekin, T.; Krupka, H.; Cho, U.S.; Kim, M.Y.; So, M.; Kim, C.Y.; Naranjo, C.M.; Rogers, Y.C.; Park, M.S.; Waldo, G.S.; Pashkov, I.; Cascio, D.; Perry, J.L.; Sawaya, M.R.
Crystal structure of the Mycobacterium tuberculosis dUTPase: insights into the catalytic mechanism (2004), J. Mol. Biol., 341, 503-517.

Search for more literature for 3.6.1.23

Application

Application	Comment	Organism
medicine	potential target for anti-tuberculosis therapy	Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-Gold (DE3) as His-tag fusion protein	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging and sitting drop vapor diffusion method	Mycobacterium tuberculosis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for stabilization of the active form of the enzyme	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dUTP + H2O	Mycobacterium tuberculosis	removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA	dUMP + diphosphate	-	?
dUTP + H2O	Mycobacterium tuberculosis H37Rv	removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA	dUMP + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WNS5	-	-
Mycobacterium tuberculosis H37Rv	P9WNS5	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein using His-tag	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dUTP + H2O	-	Mycobacterium tuberculosis	dUMP + diphosphate	-	?
dUTP + H2O	removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA	Mycobacterium tuberculosis	dUMP + diphosphate	-	?
dUTP + H2O	-	Mycobacterium tuberculosis H37Rv	dUMP + diphosphate	-	?
dUTP + H2O	removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA	Mycobacterium tuberculosis H37Rv	dUMP + diphosphate	-	?

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Release 2023.1 (January 2023)