Cloned (Comment) | Organism |
---|---|
expressed in different Escherichia coli strains | Drosophila melanogaster |
Crystallization (Comment) | Organism |
---|---|
hanging and sitting drop vapor diffusion method, stable monomers observed in crystal phase | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
DELTA160-187 | truncated form | Drosophila melanogaster |
General Stability | Organism |
---|---|
dUMP protects against tryptic digestion, slight fragmentation caused by frequent freezing and thawing that does not affect the activity | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dUDP | - |
Drosophila melanogaster | |
dUMP | - |
Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
dUTP | pH 7.5, 25°C | Drosophila melanogaster | |
0.64 | - |
dTTP | pH 7.5, 25°C | Drosophila melanogaster | |
2.3 | - |
dCTP | pH 7.5, 25°C | Drosophila melanogaster |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, modulates conformation of the enzyme | Drosophila melanogaster |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17200 | - |
3 * 19800, full length enzyme, 3 * 17200, truncated enzyme, mass spectrometry, gel filtration | Drosophila melanogaster |
19800 | - |
3 * 19800, full length enzyme, 3 * 17200, truncated enzyme, mass spectrometry, gel filtration | Drosophila melanogaster |
54000 | - |
truncated enzyme, gel filtration | Drosophila melanogaster |
65000 | - |
full length enzyme, gel filtration | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | Drosophila melanogaster | removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, provides substrate for dTTP synthesis | dUMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Drosophila melanogaster |
Storage Stability | Organism |
---|---|
-70°C, stable for at least 3 months | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dCTP + H2O | - |
Drosophila melanogaster | dCMP + diphosphate | - |
? | |
dTTP + H2O | - |
Drosophila melanogaster | dTMP + diphosphate | - |
? | |
dUTP + H2O | - |
Drosophila melanogaster | dUMP + diphosphate | - |
? | |
dUTP + H2O | removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, provides substrate for dTTP synthesis | Drosophila melanogaster | dUMP + diphosphate | - |
? | |
additional information | no hydrolysis of dUDP | Drosophila melanogaster | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 19800, full length enzyme, 3 * 17200, truncated enzyme, mass spectrometry, gel filtration | Drosophila melanogaster |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
54 | - |
melting point | Drosophila melanogaster |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
dTTP | pH 7.5, 25°C | Drosophila melanogaster | |
0.7 | - |
dCTP | pH 7.5, 25°C | Drosophila melanogaster | |
12 | - |
dUTP | pH 7.5, 25°C | Drosophila melanogaster |