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Literature summary for 3.6.1.23 extracted from

  • Williams, M.V.
    Deoxyuridine triphosphate nucleotidohydrolase induced by herpes simplex virus type 1. Purification and characterization of induced enzyme (1984), J. Biol. Chem., 259, 10080-10084.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxymercuribenzoate 5 mM, complete inhibition Homo sapiens
EDTA reversed by Mg2+, Co2+ or Mn2+ Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
dUTP enzyme from uninfected KB cells Homo sapiens
0.0048
-
dUTP enzyme from herpes simplex virus type 1 infected KB cells Human alphaherpesvirus 1

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulates, enzyme also exhibits activity in absence of divalent cations Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
enzyme from uninfected KB cells, gel filtration Homo sapiens
53000
-
enzyme from HSV-1 infected KB cells, gel filtration Human alphaherpesvirus 1

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
HSV-1-induced dUTPase and KB dUTPase
-
Human alphaherpesvirus 1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa-S3 cell
-
Homo sapiens
-
KB cell KB cells induced or not induced with herpes simplex virus type 1 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.46
-
enzyme from uninfected KB cells Homo sapiens
2.171
-
enzyme from HSV-1-infected cells Human alphaherpesvirus 1

Storage Stability

Storage Stability Organism
-20°C, 7 days, 2% loss of activity of HSV-1 induced dUTPase Human alphaherpesvirus 1
-20°C, 7 days, 70% loss of activity of uninduced dUTPase Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dUTP + H2O
-
Homo sapiens dUMP + diphosphate
-
?
dUTP + H2O
-
Human alphaherpesvirus 1 dUMP + diphosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
t1/2: 35 min for enzyme from HSV-1 infected KB cells Human alphaherpesvirus 1
45
-
t1/2: 22 min for enzyme from uninfected KB cells Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 enzyme from HSV-1 infected KB cells Human alphaherpesvirus 1
7 8 enzyme from uninfected KB cells Homo sapiens