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Literature summary for 3.6.1.23 extracted from
- Purification and properties of the deoxyuridine triphosphate nucleotidohydrolase enzyme derived from HeLa S3 cells. Comparison to a distinct dUTP nucleotidohydrolase induced in herpes simplex virus-infected HeLa S3 cells (1984), J. Biol. Chem., 259, 5459-5464.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | dissociation of the enzyme into its monomeric form | Homo sapiens |  |
| EGTA | - |
Homo sapiens | |
| Mn2+ | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
dUTP | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dialyzed enzyme is activated 2fold by 5 mM MgCl2, reverses inhibition by EDTA | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22500 | - |
2 * 22500, SDS-PAGE | Homo sapiens |
| 35000 | - |
1 * 35000, virally induced enzyme, SDS-PAGE | Human alphaherpesvirus 1 |
| 45000 | - |
gel filtration, density gradient centrifugation in presence of MgCl2 | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
after infection with herpes simplex type 1 virus a distinct species of dUTPase is induced in HeLa S3 cells | - |
| Human alphaherpesvirus 1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
| KB cell | - |
Homo sapiens | - |
| RAJI cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dUTP + H2O | - |
Homo sapiens | dUMP + diphosphate | - |
? | |
| dUTP + H2O | - |
Human alphaherpesvirus 1 | dUMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 22500, SDS-PAGE | Homo sapiens |
| monomer | 1 * 35000, virally induced enzyme, SDS-PAGE | Human alphaherpesvirus 1 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.17 | - |
dUTP | - |
Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | 20 mM potassium phosphate buffer | Homo sapiens |
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