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Literature summary for 3.6.1.22 extracted from

  • Frick, D.N.; Bessman, M.J.
    Cloning, purification, and properties of a novel NADH pyrophosphatase (1995), J. Biol. Chem., 270, 1529-1534.
    View publication on PubMed
Search for more literature for 3.6.1.22

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
NMNH competitive inhibitor with Ki: 4.2 mM, no inhibition with NMN+ Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 NADH in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5 Escherichia coli
0.29
-
 deamino-NADH in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5 Escherichia coli
0.67
-
 AppA in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5 Escherichia coli
1.8
-
 ADP-ribose in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5 Escherichia coli
2.6
-
 deamino-NAD+ in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5 Escherichia coli
5.1
-
 NAD+ in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ slight activation Escherichia coli
Fe2+ slight activation Escherichia coli
Mg2+ strong activation Escherichia coli
Mn2+ strong activation Escherichia coli
Zn2+ slight activation Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29774
-
 homodimer, 2 * 29774 in SDS-PAGE, gel filtration Escherichia coli
60000
-
 gel filtration, Sephadex G-100 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H2O Escherichia coli possible function in regulation of NAD+/NADH-ratio NMNH + AMP
-
 ir

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
more than 98% purity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-glucose + H2O
-
 Escherichia coli ?
-
 ?
ADPribose + H2O
-
 Escherichia coli ?
-
 ?
AppA + H2O
-
 Escherichia coli ?
-
 ?
ApppA + H2O
-
 Escherichia coli ?
-
 ?
deamino-NAD+ + H2O
-
 Escherichia coli deamino-NMN + AMP
-
 ?
deamino-NADH + H2O
-
 Escherichia coli deamino-NMNH + AMP
-
 ?
FAD + H2O
-
 Escherichia coli ?
-
 ?
NAD+ + H2O
-
 Escherichia coli NMN + AMP
-
 ir
NADH + H2O possible function in regulation of NAD+/NADH-ratio Escherichia coli NMNH + AMP
-
 ir
NADH + H2O
-
 Escherichia coli AMP + NMNH
-
 ?
NADP+ + H2O
-
 Escherichia coli NMN + adenosine 3',5'-diphosphate
-
 ?
NADPH + H2O
-
 Escherichia coli ?
-
 ?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
 Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
dimer homodimer, 2 * 29774 in SDS-PAGE, gel filtration Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 NADH based on two active sites/dimer Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1
-
 NMNH competitive inhibitor, no inhibiton with NMN+ Escherichia coli