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Literature summary for 3.6.1.20 extracted from

  • Kellerman, G.M.
    Isolation and characteristics of the enzyme acyl 5'-nucleotidase (1959), Biochim. Biophys. Acta, 33, 101-105.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
adenylic acid 50 mM causes 20% inhibition Sus scrofa
iodoacetate inhibitory above 10 mM Sus scrofa
p-chloromercuribenzoate inhibitory above 0.1 mM Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Sus scrofa 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-acylphosphoadenosine + H2O Sus scrofa
-
AMP + carboxylate
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Sus scrofa
-

Storage Stability

Storage Stability Organism
-18°C, stable for months, 30% loss of activity by frequent freezing and thawing within 2 month Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-acylphosphoadenosine + H2O
-
Sus scrofa AMP + carboxylate
-
?
acetyl adenylate + H2O
-
Sus scrofa acetic acid + AMP
-
?
benzoyl adenylate + H2O
-
Sus scrofa benzoic acid + AMP
-
?
hippuryl adenylate + H2O
-
Sus scrofa hippuric acid + AMP
-
?
inosine 5'-phosphobutyrate + H2O
-
Sus scrofa butyric acid + IMP
-
?
n-butyryl adenylate + H2O
-
Sus scrofa n-butanoic acid + AMP
-
?
uridine 5'-phosphobutyrate + H2O
-
Sus scrofa butyric acid + UMP
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
in 0.1 M potassium phosphate Sus scrofa