Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Aquifex aeolicus |
Crystallization (Comment) | Organism |
---|---|
free and ATP-bound enzyme, hanging drop vapor diffusion method, using 0.1 M Tris-HCl pH 7.6, 29% (w/v) PEG 3350, and 0.75 M NaCl (free enzyme) or 1 ml 0.1 M Tris-HCl pH 8.8, 25% (w/v) PEG 3350, and 0.2 M NaCl (ATP-bound enzyme) | Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C | Aquifex aeolicus | |
0.089 | - |
P1,P6-bis(5'-adenosyl) hexaphosphate | in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C | Aquifex aeolicus | |
0.183 | - |
P1,P5-bis(5'-adenosyl) pentaphosphate | in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C | Aquifex aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O66548 | - |
- |
Purification (Comment) | Organism |
---|---|
Toyopearl SuperQ-650M column chromatography, Resource Q column chromatography, Bio-Scale CHT2-I column chromatography, and Superdex 75 gel filtration | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | best substrate | Aquifex aeolicus | ATP + AMP | - |
? | |
P1,P5-bis(5'-adenosyl) pentaphosphate + H2O | - |
Aquifex aeolicus | ATP + ADP | - |
? | |
P1,P6-bis(5'-adenosyl) hexaphosphate + H2O | - |
Aquifex aeolicus | ATP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ap4A hydrolase | - |
Aquifex aeolicus |
asymmetric Ap4A hydrolase | - |
Aquifex aeolicus |
asymmetric diadenosine tetraphosphate hydrolase | - |
Aquifex aeolicus |
ATP-producing Ap4A hydrolase | - |
Aquifex aeolicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
P1,P6-bis(5'-adenosyl) hexaphosphate | in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C | Aquifex aeolicus | |
1.4 | - |
P1,P5-bis(5'-adenosyl) pentaphosphate | in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C | Aquifex aeolicus | |
2.5 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C | Aquifex aeolicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
P1,P6-bis(5'-adenosyl) hexaphosphate | in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C | Aquifex aeolicus | |
7.6 | - |
P1,P5-bis(5'-adenosyl) pentaphosphate | in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C | Aquifex aeolicus | |
46 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C | Aquifex aeolicus |