Literature summary for 3.6.1.17 extracted from

Szurmak, B.; Wyslouch-Cieszyeska, A.; Wszelaka-Rylik, M.; Bal, W.; Dobrzanska, M.
A diadenosine 5',5''-P1P4 tetraphosphate (Ap4A) hydrolase from Arabidopsis thaliana that is activated preferentially by Mn2+ ions (2008), Acta Biochim. Pol., 55, 151-160.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Arabidopsis thaliana

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	no activity in the presence of 1.0 and 5.0 mM Ca2+	Arabidopsis thaliana
Cd2+	no activity in the presence of 1.0 mM Cd2+, weak enzyme activation at 5 mM (4.% compared to 1.5 mM Mn2+)	Arabidopsis thaliana
Mg2+	no activity in the presence of 1 mM Mg2+, 9.1% relative activity at 5 mM Mg2+ compared to 1.5 mM Mn2+	Arabidopsis thaliana
Mn2+	enzyme preferentially hydrolyses diadenosine 5',5'-P1P4 tetraphosphate in the form of a Mn2+ complex at a concentration of 1.5 mM, 37.7% relative activity at 0.5 mM compared to 1.5 mM Mn2+	Arabidopsis thaliana
Zn2+	enzyme exhibit residual activity in the presence of 1.5 mM Zn2+ ions, 28.4% relative activity at 5 mM Zn2+ compared to 1.5 mM Mn2+	Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
21670	-	by electrospray ionization quadrupole time-of-flight mass spectrometry	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
diadenosine 5',5'-P1,P4-tetraphosphate + H2O	Arabidopsis thaliana	key enzyme controlling the in vivo concentration of diadenosine 5',5'-P1,P4 tetraphosphate (Ap4A) - an important signaling molecule involved in regulation of DNA replication and repair, signaling in stress response and apoptosis	ATP + AMP + ?	-	?
diadenosine 5',5'-P1,P5-pentaphosphate + H2O	Arabidopsis thaliana	-	ATP + ADP	-	?
diguanosine 5',5'-P1,P4-tetraphosphate + H2O	Arabidopsis thaliana	-	GTP + GMP	-	?
additional information	Arabidopsis thaliana	enzyme hydrolyses bis(5'-nucleosidyl) polyphosphates (Np4-6N') in such a way that a nucleosidyl triphosphate is always one of the products	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9C6Z2	-	-

Purification (Commentary)

Purification (Comment)	Organism
by affinity chromatography by means of nickel-nitrilotriacetic acid agarose	Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5.9	-	Mn2+ concentration 0.15 mM	Arabidopsis thaliana
19.2	-	Mn2+ concentration 0.75 mM	Arabidopsis thaliana
27	-	maximal activity of the enzyme coincides with total Mn2+ and Ap4A concentrations of 1 mM, at such concentrations the Mn(Ap4A) complex dominates and the concentration of its components Mn2+ = Ap4A are 0.083 mM, an excess of either Mn2+ or Ap4A over Mn(Ap4A) results in a decrease of the reaction rate	Arabidopsis thaliana
31.1	-	Mn2+ concentration 1.5 mM	Arabidopsis thaliana
55.2	-	Mn2+ concentration 3.5 mM	Arabidopsis thaliana
70.5	-	Mn2+ concentration 6.5 mM	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
diadenosine 5',5'-P1,P4-tetraphosphate + H2O	key enzyme controlling the in vivo concentration of diadenosine 5',5'-P1,P4 tetraphosphate (Ap4A) - an important signaling molecule involved in regulation of DNA replication and repair, signaling in stress response and apoptosis	Arabidopsis thaliana	ATP + AMP + ?	-	?
diadenosine 5',5'-P1,P5-pentaphosphate + H2O	-	Arabidopsis thaliana	ATP + ADP	-	?
diguanosine 5',5'-P1,P4-tetraphosphate + H2O	-	Arabidopsis thaliana	GTP + GMP	-	?
additional information	enzyme hydrolyses bis(5'-nucleosidyl) polyphosphates (Np4-6N') in such a way that a nucleosidyl triphosphate is always one of the products	Arabidopsis thaliana	?	-	?

Synonyms

Synonyms	Comment	Organism
Ap4A hydrolase	-	Arabidopsis thaliana
asymmetrical bis-(5'-nucleosidyl)-tetraphosphatase	-	Arabidopsis thaliana
AtNUDX25 protein	-	Arabidopsis thaliana
diadenosine 5',5'-P1P4 tetraphosphate hydrolase	-	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Arabidopsis thaliana

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.8	9.5	in the presence of 3.5 mM Mn2+, the enzyme is active over a wide pH range	Arabidopsis thaliana

pI Value

Organism	Comment	pI Value Maximum	pI Value
Arabidopsis thaliana	deduced pI from amino acid sequence	-	4.1

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Release 2023.1 (January 2023)