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Literature summary for 3.6.1.17 extracted from
- Human placental (asymmetrical) diadenosine 5',5'''-P1,P4-tetraphosphate hydrolase: Purification to homogenity and some properties (1993), Protein Expr. Purif., 4, 45-51.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine 5'-tetraphosphate | Ki: 0.006 mM | Homo sapiens |  |
| Ca2+ | Ki: 0.3 mM | Homo sapiens | |
| diadenosine triphosphate | competitive, also phosphonate analogues of the substance | Homo sapiens | |
| fluoride | Ki: 0.02 mM | Homo sapiens | |
| guanosine 5'-tetraphosphate | Ki: 0.01 mM | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | in presence of Mg2+ | Homo sapiens | |
| 0.04 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | in presence of Zn2+ | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 60% of activity | Homo sapiens | |
| Mg2+ | maximum activity at 3 mM | Homo sapiens | |
| Mn2+ | 40% of activity | Homo sapiens | |
| Zn2+ | 50% of activity | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19200 | - |
SDS-PAGE, gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | Homo sapiens | - |
ATP + AMP | - |
? | |
| P1,P4-bis(5'-guanosyl)tetraphosphate + H2O | Homo sapiens | - |
GTP + GMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00022 | - |
crude extract | Homo sapiens |
| 52 | - |
purified enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| di-2,6-diaminopurine beta-D-ribofuranoside tetraphosphate + H2O | - |
Homo sapiens | ? | - |
? | |
| P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | - |
Homo sapiens | ATP + AMP | - |
? | |
| P1,P4-bis(5'-guanosyl)tetraphosphate + H2O | - |
Homo sapiens | GTP + GMP | - |
? | |
| P1,P5-bis(5'-adenosyl) pentaphosphate + H2O | - |
Homo sapiens | ADP + ATP | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | half maximum activity | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
guanosine 5'-tetraphosphate | - |
Homo sapiens | |
| 0.02 | - |
fluoride | - |
Homo sapiens | |
| 0.3 | - |
Ca2+ | - |
Homo sapiens | |
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