Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | stimulating in physiological concentrations, 148% relative activity at a concentration of 0.5 mM, 128% relative activity at a concentration of 0.05 mM | Bos taurus | |
acetyl-CoA | stimulating in physiological concentrations, 148% relative activity at a concentration of 0.5 mM, 127% relative activity at a concentration of 0.05 mM | Bos taurus | |
D-fructose 1,6-bisphosphate | stimulating in physiological concentrations, 155% relative activity at a concentration of 0.5 mM, 126% relative activity at a concentration of 0.05 mM | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
propionate | 39% relative activity at a concentration of 0.5 mM, 63% relative activity at a concentration of 0.05 mM | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
ITP | in presence of 10 mM Mg2+ | Bos taurus | |
0.041 | - |
GTP | in presence of 10 mM Mg2+ | Bos taurus | |
0.086 | - |
UTP | in presence of 10 mM Mg2+ | Bos taurus | |
0.15 | - |
CTP | in presence of 10 mM Mg2+ | Bos taurus | |
0.26 | - |
ATP | in presence of 10 mM Mg2+ | Bos taurus | |
0.5 | - |
CDP | in presence of 10 mM Mg2+ | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bos taurus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 5 mM, unable to stimulate enzyme activity | Bos taurus | |
Ca2+ | 5 mM, 52% relative enzyme activity in presence of | Bos taurus | |
Co2+ | 5 mM, 60% relative enzyme activity in presence of | Bos taurus | |
Cu2+ | 5 mM, 46% relative enzyme activity in presence of | Bos taurus | |
Mg2+ | 5 mM, 100% relative enzyme activity in presence of | Bos taurus | |
Mn2+ | 5 mM, 73% relative enzyme activity in presence of | Bos taurus | |
Zn2+ | 5 mM, 34% relative enzyme activity in presence of | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58600 | - |
gel filtration, at 4°C in 20 mM Tris-HCl, 0.1 M NaCl, pH 7.5 | Bos taurus |
60200 | - |
SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | Bos taurus | 0.5 mM, 25% relative activity towards ADP compared to CTP, no activity towards AMP | AMP + phosphate | - |
? | |
ATP + H2O | Bos taurus | 0.5 mM, 32% relative activity compared to CTP | ADP + phosphate | - |
? | |
CDP + H2O | Bos taurus | 0.5 mM, 41% relative activity towards CDP, no activity towards CMP | CMP + phosphate | - |
? | |
CTP + H2O | Bos taurus | 0.5 mM, 100% relative activity | CDP + phosphate | - |
? | |
GDP + H2O | Bos taurus | 0.5 mM, 19% relative activity towards GDP compared to CTP, no activity towards GMP | GMP + phosphate | - |
? | |
GTP + H2O | Bos taurus | 0.5 mM, 30% relative activity compared to CTP | GDP + phosphate | - |
? | |
IDP + H2O | Bos taurus | 0.5 mM, 41% relative activity towards IDP compared to CTP, 5.3% relative activity towards IMP compared to CTP | IMP + phosphate | - |
? | |
ITP + H2O | Bos taurus | 0.5 mM, 58% relative activity compared to CTP | IDP + phosphate | - |
? | |
UDP + H2O | Bos taurus | 0.5 mM, 32% relative activity compared to CTP, 8.2% relative activity towards UMP compared to CTP | UMP + phosphate | - |
? | |
UTP + H2O | Bos taurus | 0.5 mM, 66% relative activity compared to CTP | UDP + phosphate | - |
? | |
XTP + H2O | Bos taurus | 0.5 mM, 40% relative activity compared to CTP | XDP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration, SDS-PAGE | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | purified from | Bos taurus | - |
heart | expression in | Bos taurus | - |
kidney | expression in | Bos taurus | - |
liver | expression in | Bos taurus | - |
lung | expression in | Bos taurus | - |
small intestine | expression in | Bos taurus | - |
spleen | expression in | Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
dependence on bivalent cations and broad substrate specificity towards nucleoside-5'-tri- and -diphosphates reveal similarities to apyrases | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | 0.5 mM, 25% relative activity towards ADP compared to CTP, no activity towards AMP | Bos taurus | AMP + phosphate | - |
? | |
ATP + H2O | 0.5 mM, 32% relative activity compared to CTP | Bos taurus | ADP + phosphate | - |
? | |
CDP + H2O | 0.5 mM, 41% relative activity towards CDP, no activity towards CMP | Bos taurus | CMP + phosphate | - |
? | |
CDP + H2O | 0.5 mM, 41% relative activity towards CDP compared to CTP, no activity towards CMP | Bos taurus | CMP + phosphate | - |
? | |
CTP + H2O | 0.5 mM, 100% relative activity | Bos taurus | CDP + phosphate | - |
? | |
GDP + H2O | 0.5 mM, 19% relative activity towards GDP compared to CTP, no activity towards GMP | Bos taurus | GMP + phosphate | - |
? | |
GTP + H2O | 0.5 mM, 30% relative activity compared to CTP | Bos taurus | GDP + phosphate | - |
? | |
IDP + H2O | 0.5 mM, 41% relative activity towards IDP compared to CTP, 5.3% relative activity towards IMP compared to CTP | Bos taurus | IMP + phosphate | - |
? | |
IDP + H2O | 0.5 mM, 41% relative activity towards IDP, 5.3% relative activity towards IMP compared to CTP | Bos taurus | IMP + phosphate | - |
? | |
ITP + H2O | 0.5 mM, 58% relative activity compared to CTP | Bos taurus | IDP + phosphate | - |
? | |
UDP + H2O | 0.5 mM, 32% relative activity compared to CTP, 8.2% relative activity towards UMP compared to CTP | Bos taurus | UMP + phosphate | - |
? | |
UDP + H2O | 0.5 mM, 32% relative activity, 8.2% relative activity towards UMP compared to CTP | Bos taurus | UMP + phosphate | - |
? | |
UTP + H2O | 0.5 mM, 66% relative activity compared to CTP | Bos taurus | UDP + phosphate | - |
? | |
XTP + H2O | 0.5 mM, 40% relative activity compared to CTP | Bos taurus | XDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60200, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
nucleoside triphosphatase | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | pH 6.0: about 55% of maximal activity, pH 8.5: about 55% of maximal activity | Bos taurus |