Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Poly(U) | the activation by poly(U) is inhibited by K+ | Classical swine fever virus |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, functional expression of His-tagged full-length viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3 in Escherichia coli | Classical swine fever virus |
Protein Variants | Comment | Organism |
---|---|---|
A354V | site-directed mutagenesis of the motif III residue does not affect the enzyme activity and kinetics | Classical swine fever virus |
K232A | site-directed mutagenesis of the conserved motif I residue leads to inactivation of the mutant | Classical swine fever virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Classical swine fever virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, activates | Classical swine fever virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, recombinant full-length NS3 protein, SDS-PAGE | Classical swine fever virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Classical swine fever virus | - |
ADP + phosphate | - |
? | |
ATP + H2O | Classical swine fever virus CSFV | - |
ADP + phosphate | - |
? | |
CTP + H2O | Classical swine fever virus | - |
CDP + phosphate | - |
? | |
dATP + H2O | Classical swine fever virus | - |
dADP + phosphate | - |
? | |
dCTP + H2O | Classical swine fever virus | - |
dCDP + phosphate | - |
? | |
dGTP + H2O | Classical swine fever virus | - |
dGDP + phosphate | - |
? | |
dUTP + H2O | Classical swine fever virus | - |
dUDP + phosphate | - |
? | |
GTP + H2O | Classical swine fever virus | - |
GDP + phosphate | - |
? | |
GTP + H2O | Classical swine fever virus CSFV | - |
GDP + phosphate | - |
? | |
additional information | Classical swine fever virus | the viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3, recombinantly expressed in Escherichia coli, contain specific polynucleotide-stimulated NTPase acitivity, the enzyme activity is essential for viral replication | ? | - |
? | |
additional information | Classical swine fever virus CSFV | the viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3, recombinantly expressed in Escherichia coli, contain specific polynucleotide-stimulated NTPase acitivity, the enzyme activity is essential for viral replication | ? | - |
? | |
UTP + H2O | Classical swine fever virus | - |
UDP + phosphate | - |
? | |
UTP + H2O | Classical swine fever virus CSFV | - |
UDP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Classical swine fever virus | - |
CSFV | - |
Classical swine fever virus CSFV | - |
CSFV | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged full-length viral NS3 protein and N-terminal truncated version ntNS3 from Escherichia coli by nickel affinity chromatography and dialysis | Classical swine fever virus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Classical swine fever virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Classical swine fever virus | ADP + phosphate | - |
? | |
ATP + H2O | - |
Classical swine fever virus CSFV | ADP + phosphate | - |
? | |
CTP + H2O | - |
Classical swine fever virus | CDP + phosphate | - |
? | |
dATP + H2O | - |
Classical swine fever virus | dADP + phosphate | - |
? | |
dCTP + H2O | - |
Classical swine fever virus | dCDP + phosphate | - |
? | |
dGTP + H2O | - |
Classical swine fever virus | dGDP + phosphate | - |
? | |
dUTP + H2O | - |
Classical swine fever virus | dUDP + phosphate | - |
? | |
GTP + H2O | - |
Classical swine fever virus | GDP + phosphate | - |
? | |
GTP + H2O | - |
Classical swine fever virus CSFV | GDP + phosphate | - |
? | |
additional information | the viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3, recombinantly expressed in Escherichia coli, contain specific polynucleotide-stimulated NTPase acitivity, the enzyme activity is essential for viral replication | Classical swine fever virus | ? | - |
? | |
additional information | the conserved motif I, GXGK232T, is required for NTPase activity of the viral NS3 protein, while motif III is not | Classical swine fever virus | ? | - |
? | |
additional information | the viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3, recombinantly expressed in Escherichia coli, contain specific polynucleotide-stimulated NTPase acitivity, the enzyme activity is essential for viral replication | Classical swine fever virus CSFV | ? | - |
? | |
additional information | the conserved motif I, GXGK232T, is required for NTPase activity of the viral NS3 protein, while motif III is not | Classical swine fever virus CSFV | ? | - |
? | |
UTP + H2O | - |
Classical swine fever virus | UDP + phosphate | - |
? | |
UTP + H2O | - |
Classical swine fever virus CSFV | UDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, recombinant full-length NS3 protein, SDS-PAGE | Classical swine fever virus |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme is a nonstructural protein 3, NS3 of family Flaviviridae members | Classical swine fever virus |
NS3 protein | - |
Classical swine fever virus |
NS3FL | - |
Classical swine fever virus |
NTPase | - |
Classical swine fever virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Classical swine fever virus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
82 | - |
dUTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) | Classical swine fever virus | |
114 | - |
UTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) | Classical swine fever virus | |
135 | - |
dCTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) | Classical swine fever virus | |
136 | - |
dGTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) | Classical swine fever virus | |
155 | - |
ATP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) | Classical swine fever virus | |
208 | - |
dATP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) | Classical swine fever virus | |
220 | - |
CTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) | Classical swine fever virus | |
400 | - |
GTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) | Classical swine fever virus | |
1875 | - |
dUTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) | Classical swine fever virus | |
2162 | - |
UTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) | Classical swine fever virus | |
2286 | - |
CTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) | Classical swine fever virus | |
2500 | - |
dGTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) | Classical swine fever virus | |
2963 | - |
GTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) | Classical swine fever virus | |
3150 | - |
dATP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) | Classical swine fever virus | |
4384 | - |
ATP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) | Classical swine fever virus | |
5096 | - |
dCTP | pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) | Classical swine fever virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | broad range optimum, pH profile | Classical swine fever virus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | pH profile | Classical swine fever virus |