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Literature summary for 3.6.1.15 extracted from

  • Xu, W.; Jones, C.R.; Dunn, C.A.; Bessman, M.J.
    Gene ytkD of Bacillus subtilis encodes an atypical nucleoside triphosphatase member of the Nudix hydrolase superfamily (2004), J. Bacteriol., 186, 8380-8384.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene ytkD, expression in Escherichia coli strain HMS174, complementation of the mutT mutator phenotype in Escherichia coli strain SB3 lacking a functional mutT gene Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
gene ytkD
-
Bacillus subtilis 168
-
gene ytkD
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain HMS174 by streptomycin sulfate treatment and gel filtration Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dNTP + H2O
-
Bacillus subtilis dNDP + phosphate
-
?
dNTP + H2O
-
Bacillus subtilis 168 dNDP + phosphate
-
?

Synonyms

Synonyms Comment Organism
More the enzyme is a member of the Nudix hydrolase superfamily Bacillus subtilis
nucleoside triphosphatase
-
Bacillus subtilis
YtkD
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Bacillus subtilis