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Literature summary for 3.6.1.15 extracted from
- Polypeptide p41 of a Norwalk-like virus is a nucleic acid-independent nucleoside triphosphatase (2001), J. Virol., 75, 1611-1619.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | in presence of Mn2+, 10 mM MgCl2 inhibits 20% | Southampton virus |  |
| MgCl2 | in presence of Mn2+, 10 mM MgCl2 inhibits 10% | Southampton virus | |
| NaCl | 100 mM, inhibits ATP hydrolysis by 30% | Southampton virus | |
| polyA | inhibits to a lesser extent than polyC and polyU | Southampton virus | |
| polyC | 1 mg/l, 70% inhibition | Southampton virus | |
| polyG | inhibits to a lesser extent than polyC and polyU | Southampton virus | |
| polyU | 1 mg/l, 70% inhibition | Southampton virus | |
| RNA | homopolymeric RNA inhibits under otherwise optimal conditions | Southampton virus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 20 mM, about 50% of the maximal activation obtained with Mn2+ | Southampton virus | |
| Mn2+ | activates, 3.5-5 mM MnCl2 are optimal at an ATP concentration of 0.5 mM | Southampton virus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Southampton virus | Q04544 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Southampton virus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | viral protein is expressed in Escherichia coli cells | Southampton virus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Southampton virus | ADP + phosphate | - |
? | |
| CTP + H2O | about 35% of activity with ATP | Southampton virus | CDP + phosphate | - |
? | |
| GTP + H2O | about 90% of activity with ATP | Southampton virus | GDP + phosphate | - |
? | |
| additional information | the enzyme does not possess helicase activity | Southampton virus | ? | - |
? | |
| UTP + H2O | about 35% of activity with ATP | Southampton virus | UDP + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nucleic acid-independent nucleoside triphosphatase | - |
Southampton virus |
| polypeptide p41 | - |
Southampton virus |
| SHVp41 | - |
Southampton virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | - |
- |
Southampton virus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
ATP | - |
Southampton virus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 7.5 | ATPase activity | Southampton virus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8 | pH 6.8: about 90% of maximal activity, pH 8.0: about 70% of maximal activity | Southampton virus |
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