BRENDA - Enzyme Database show
show all sequences of 3.6.1.14

Adenosine 5'-tetraphosphate phosphohydrolase from yellow lupin seeds: purification to homogeneity and some properties

Guranowski, A.; Starzynska, E.; Brown, P.; Blackburn, G.M.; Biochem. J. 328, 257-262 (1997)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
-
Lupinus luteus
F-
-
Lupinus luteus
Zn2+
-
Lupinus luteus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
adenosine 5'-tetraphosphate
-
Lupinus luteus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
divalent metal ion, Mg2+, Co2+, Ni2+ or Mn2+, required
Lupinus luteus
Mg2+
divalent metal ion, Mg2+, Co2+, Ni2+ or Mn2+ required
Lupinus luteus
Mn2+
divalent metal ion, Mg2+, Co2+, Ni2+ or Mn2+ required
Lupinus luteus
Ni2+
divalent metal ion, Mg2+, Co2+, Ni2+ or Mn2+ required
Lupinus luteus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
gel filtration
Lupinus luteus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lupinus luteus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Lupinus luteus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seed
-
Lupinus luteus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
20.83
-
-
Lupinus luteus
Storage Stability
Storage Stability
Organism
-20°C, 6 months, no loss of activity
Lupinus luteus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adenosine 5'-pentaphosphate + H2O
hydrolyzed 18fold more slowly than adenosine 5'-tetraphosphate
209873
Lupinus luteus
?
-
-
-
?
adenosine 5'-pentaphosphate + H2O
hydrolyzed at 1/200 the rate of adenosine 5'-tetraphosphate
209873
Lupinus luteus
?
-
-
-
?
adenosine 5'-tetraphosphate + H2O
-
209873
Lupinus luteus
ATP + phosphate
-
209873
Lupinus luteus
?
guanosine tetraphosphate + H2O
-
209873
Lupinus luteus
GTP + phosphate
-
209873
Lupinus luteus
?
tripolyphosphate + H2O
-
209873
Lupinus luteus
diphosphate + phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 25000, SDS-PAGE
Lupinus luteus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.2
-
-
Lupinus luteus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.6
9.4
50% of maximal activity at pH 7.6 and 9.4
Lupinus luteus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
additional information
-
enzyme is stable at the extremes of pH for at least 30 min
Lupinus luteus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
-
Lupinus luteus
F-
-
Lupinus luteus
Zn2+
-
Lupinus luteus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
adenosine 5'-tetraphosphate
-
Lupinus luteus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
divalent metal ion, Mg2+, Co2+, Ni2+ or Mn2+, required
Lupinus luteus
Mg2+
divalent metal ion, Mg2+, Co2+, Ni2+ or Mn2+ required
Lupinus luteus
Mn2+
divalent metal ion, Mg2+, Co2+, Ni2+ or Mn2+ required
Lupinus luteus
Ni2+
divalent metal ion, Mg2+, Co2+, Ni2+ or Mn2+ required
Lupinus luteus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
gel filtration
Lupinus luteus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Lupinus luteus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seed
-
Lupinus luteus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
20.83
-
-
Lupinus luteus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 6 months, no loss of activity
Lupinus luteus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adenosine 5'-pentaphosphate + H2O
hydrolyzed 18fold more slowly than adenosine 5'-tetraphosphate
209873
Lupinus luteus
?
-
-
-
?
adenosine 5'-pentaphosphate + H2O
hydrolyzed at 1/200 the rate of adenosine 5'-tetraphosphate
209873
Lupinus luteus
?
-
-
-
?
adenosine 5'-tetraphosphate + H2O
-
209873
Lupinus luteus
ATP + phosphate
-
209873
Lupinus luteus
?
guanosine tetraphosphate + H2O
-
209873
Lupinus luteus
GTP + phosphate
-
209873
Lupinus luteus
?
tripolyphosphate + H2O
-
209873
Lupinus luteus
diphosphate + phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 25000, SDS-PAGE
Lupinus luteus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.2
-
-
Lupinus luteus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.6
9.4
50% of maximal activity at pH 7.6 and 9.4
Lupinus luteus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
additional information
-
enzyme is stable at the extremes of pH for at least 30 min
Lupinus luteus
Other publictions for EC 3.6.1.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744647
Amici
Synthesis and degradation of ...
Homo sapiens
Cell Chem. Biol.
24
553-564
2017
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668579
Guranowski
Methylene analogues of adenosi ...
Homo sapiens, Lupinus luteus
FEBS J.
273
829-838
2006
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209852
Guranowski
Adenosine 5'-tetraphosphate ph ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1380
232-238
1998
-
-
-
-
-
-
3
1
-
4
1
-
-
3
-
-
-
-
-
-
-
-
4
1
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
4
1
-
-
-
-
-
-
-
-
-
4
1
-
-
-
2
1
1
-
-
-
-
-
-
-
-
209873
Guranowski
Adenosine 5'-tetraphosphate ph ...
Lupinus luteus
Biochem. J.
328
257-262
1997
-
-
-
-
-
-
3
1
-
4
1
-
-
1
-
-
1
-
-
1
1
1
5
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
4
1
-
-
-
-
1
-
1
1
1
5
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
209871
Small
Purification and properties of ...
Oryctolagus cuniculus
Biochemistry
5
14-26
1966
-
-
-
-
-
-
3
2
-
5
-
-
-
1
-
-
1
-
-
4
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
-
5
-
-
-
-
-
1
-
4
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-