BRENDA - Enzyme Database show
show all sequences of 3.6.1.14

Purification and properties of nucleoside tetraphosphate hydrolase from rabbit muscle

Small, G.D.; Cooper, C.; Biochemistry 5, 14-26 (1966)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
N-ethylmaleimide
-
Oryctolagus cuniculus
p-hydroxymercuribenzoate
-
Oryctolagus cuniculus
tripolyphosphate
competitive inhibitor of adenosine tetraphosphate
Oryctolagus cuniculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0027
-
adenosine 5'-tetraphosphate
-
Oryctolagus cuniculus
0.0034
-
inosine tetraphosphate
-
Oryctolagus cuniculus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
divalent metal ion required, the order of decreasing effectiveness for hydrolysis of adenosine 5'-tetraphosphate is: Co2+, Fe2+, Mg2+, Ni2+, Mn2+. For tripolyphosphate hydrolysis the order is: Ni2+, Co2+, Fe2+, Mg2+, Mn2+
Oryctolagus cuniculus
Fe2+
divalent metal ion required, the order of decreasing effectiveness for hydrolysis of adenosine 5'-tetraphosphate is: Co2+, Fe2+, Mg2+, Ni2+, Mn2+. For tripolyphosphate hydrolysis the order is: Ni2+, Co2+, Fe2+, Mg2+, Mn2+
Oryctolagus cuniculus
Mg2+
divalent metal ion required, the order of decreasing effectiveness for hydrolysis of adenosine tetraphosphate is: Co2+, Fe2+, Mg2+, Ni2+, Mn2+. For tripolyphosphate hydrolysis the order is: Ni2+, Co2+, Fe2+, Mg2+, Mn2+
Oryctolagus cuniculus
Mn2+
divalent metal ion required, the order of decreasing effectiveness for hydrolysis of adenosine tetraphosphate is: Co2+, Fe2+, Mg2+, Ni2+, Mn2+. For tripolyphosphate hydrolysis the order is: Ni2+, Co2+, Fe2+, Mg2+, Mn2+
Oryctolagus cuniculus
Ni2+
divalent metal ion required, the order of decreasing effectiveness for hydrolysis of adenosine tetraphosphate is: Co2+, Fe2+, Mg2+, Ni2+, Mn2+. For tripolyphosphate hydrolysis the order is: Ni2+, Co2+, Fe2+, Mg2+, Mn2+
Oryctolagus cuniculus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Oryctolagus cuniculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
heart
-
Oryctolagus cuniculus
-
kidney
-
Oryctolagus cuniculus
-
liver
-
Oryctolagus cuniculus
-
muscle
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adenosine 5'-tetraphosphate + H2O
-
209871
Oryctolagus cuniculus
ATP + phosphate
-
209871
Oryctolagus cuniculus
?
inosine tetraphosphate + H2O
-
209871
Oryctolagus cuniculus
ITP + phosphate
-
209871
Oryctolagus cuniculus
?
tripolyphosphate + H2O
-
209871
Oryctolagus cuniculus
diphosphate + phosphate
-
209871
Oryctolagus cuniculus
?
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
N-ethylmaleimide
-
Oryctolagus cuniculus
p-hydroxymercuribenzoate
-
Oryctolagus cuniculus
tripolyphosphate
competitive inhibitor of adenosine tetraphosphate
Oryctolagus cuniculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0027
-
adenosine 5'-tetraphosphate
-
Oryctolagus cuniculus
0.0034
-
inosine tetraphosphate
-
Oryctolagus cuniculus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
divalent metal ion required, the order of decreasing effectiveness for hydrolysis of adenosine 5'-tetraphosphate is: Co2+, Fe2+, Mg2+, Ni2+, Mn2+. For tripolyphosphate hydrolysis the order is: Ni2+, Co2+, Fe2+, Mg2+, Mn2+
Oryctolagus cuniculus
Fe2+
divalent metal ion required, the order of decreasing effectiveness for hydrolysis of adenosine 5'-tetraphosphate is: Co2+, Fe2+, Mg2+, Ni2+, Mn2+. For tripolyphosphate hydrolysis the order is: Ni2+, Co2+, Fe2+, Mg2+, Mn2+
Oryctolagus cuniculus
Mg2+
divalent metal ion required, the order of decreasing effectiveness for hydrolysis of adenosine tetraphosphate is: Co2+, Fe2+, Mg2+, Ni2+, Mn2+. For tripolyphosphate hydrolysis the order is: Ni2+, Co2+, Fe2+, Mg2+, Mn2+
Oryctolagus cuniculus
Mn2+
divalent metal ion required, the order of decreasing effectiveness for hydrolysis of adenosine tetraphosphate is: Co2+, Fe2+, Mg2+, Ni2+, Mn2+. For tripolyphosphate hydrolysis the order is: Ni2+, Co2+, Fe2+, Mg2+, Mn2+
Oryctolagus cuniculus
Ni2+
divalent metal ion required, the order of decreasing effectiveness for hydrolysis of adenosine tetraphosphate is: Co2+, Fe2+, Mg2+, Ni2+, Mn2+. For tripolyphosphate hydrolysis the order is: Ni2+, Co2+, Fe2+, Mg2+, Mn2+
Oryctolagus cuniculus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Oryctolagus cuniculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
heart
-
Oryctolagus cuniculus
-
kidney
-
Oryctolagus cuniculus
-
liver
-
Oryctolagus cuniculus
-
muscle
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adenosine 5'-tetraphosphate + H2O
-
209871
Oryctolagus cuniculus
ATP + phosphate
-
209871
Oryctolagus cuniculus
?
inosine tetraphosphate + H2O
-
209871
Oryctolagus cuniculus
ITP + phosphate
-
209871
Oryctolagus cuniculus
?
tripolyphosphate + H2O
-
209871
Oryctolagus cuniculus
diphosphate + phosphate
-
209871
Oryctolagus cuniculus
?
Other publictions for EC 3.6.1.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744647
Amici
Synthesis and degradation of ...
Homo sapiens
Cell Chem. Biol.
24
553-564
2017
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668579
Guranowski
Methylene analogues of adenosi ...
Homo sapiens, Lupinus luteus
FEBS J.
273
829-838
2006
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
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-
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-
-
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-
-
-
-
-
2
-
-
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-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209852
Guranowski
Adenosine 5'-tetraphosphate ph ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1380
232-238
1998
-
-
-
-
-
-
3
1
-
4
1
-
-
1
-
-
-
-
-
-
-
-
4
1
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
4
1
-
-
-
-
-
-
-
-
-
4
1
-
-
-
2
1
1
-
-
-
-
-
-
-
-
209873
Guranowski
Adenosine 5'-tetraphosphate ph ...
Lupinus luteus
Biochem. J.
328
257-262
1997
-
-
-
-
-
-
3
1
-
4
1
-
-
1
-
-
1
-
-
1
1
1
5
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
4
1
-
-
-
-
1
-
1
1
1
5
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
209871
Small
Purification and properties of ...
Oryctolagus cuniculus
Biochemistry
5
14-26
1966
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-
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3
2
-
5
-
-
-
1
-
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1
-
-
4
1
-
3
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
3
-
2
-
5
-
-
-
-
-
1
-
4
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
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