BRENDA - Enzyme Database show
show all sequences of 3.6.1.14

Adenosine 5'-tetraphosphate phosphohydrolase activity is an inherent property of soluble exopolyphosphatase from yeast Saccharomyces cerevisiae

Guranowski, A.; Starzynska, E.; Barnes, L.D.; Robinson, A.K.; Liu, S.; Biochim. Biophys. Acta 1380, 232-238 (1998)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ammonium molybdate
-
Saccharomyces cerevisiae
sodium o-vanadate
-
Saccharomyces cerevisiae
Zn2+
ZnCl2
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.08
-
adenosine 5'-tetraphosphate
pH 4.75, 50 mM sodium acetate, 5 mM CoCl2
Saccharomyces cerevisiae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
divalent cations activate hydrolysis of adenosine 5'-tetraphosphate in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Ni2+
Saccharomyces cerevisiae
Mg2+
divalent cations activate hydrolysis of adenosine 5'-tetraphosphate in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Ni2+
Saccharomyces cerevisiae
Mn2+
divalent cations activate hydrolysis of adenosine 5'-tetraphosphate in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Ni2+
Saccharomyces cerevisiae
Ni2+
divalent cations activate hydrolysis of adenosine 5'-tetraphosphate in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Ni2+
Saccharomyces cerevisiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
x * 45000, SDS-PAGE
Saccharomyces cerevisiae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adenosine 5'-pentaphosphate + H2O
-
209852
Saccharomyces cerevisiae
?
-
-
-
?
adenosine 5'-tetraphosphate + H2O
-
209852
Saccharomyces cerevisiae
ATP + phosphate
-
209852
Saccharomyces cerevisiae
?
guanosine tetraphosphate + H2O
-
209852
Saccharomyces cerevisiae
GTP + phosphate
-
209852
Saccharomyces cerevisiae
?
inosine tetraphosphate + H2O
-
209852
Saccharomyces cerevisiae
ITP + phosphate
-
209852
Saccharomyces cerevisiae
?
Subunits
Subunits
Commentary
Organism
?
x * 45000, SDS-PAGE
Saccharomyces cerevisiae
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
40
-
adenosine-5'-pentaphosphate
-
Saccharomyces cerevisiae
723
-
adenosine 5'-tetraphosphate
-
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.8
-
-
Saccharomyces cerevisiae
pH Range
pH Minimum
pH Maximum
Commentary
Organism
3.8
7.2
50% of maximal activity at pH 3.8 and 7.2
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ammonium molybdate
-
Saccharomyces cerevisiae
sodium o-vanadate
-
Saccharomyces cerevisiae
Zn2+
ZnCl2
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.08
-
adenosine 5'-tetraphosphate
pH 4.75, 50 mM sodium acetate, 5 mM CoCl2
Saccharomyces cerevisiae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
divalent cations activate hydrolysis of adenosine 5'-tetraphosphate in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Ni2+
Saccharomyces cerevisiae
Mg2+
divalent cations activate hydrolysis of adenosine 5'-tetraphosphate in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Ni2+
Saccharomyces cerevisiae
Mn2+
divalent cations activate hydrolysis of adenosine 5'-tetraphosphate in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Ni2+
Saccharomyces cerevisiae
Ni2+
divalent cations activate hydrolysis of adenosine 5'-tetraphosphate in the order of decreasing effectiveness: Co2+, Mn2+, Mg2+, Ni2+
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
x * 45000, SDS-PAGE
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adenosine 5'-pentaphosphate + H2O
-
209852
Saccharomyces cerevisiae
?
-
-
-
?
adenosine 5'-tetraphosphate + H2O
-
209852
Saccharomyces cerevisiae
ATP + phosphate
-
209852
Saccharomyces cerevisiae
?
guanosine tetraphosphate + H2O
-
209852
Saccharomyces cerevisiae
GTP + phosphate
-
209852
Saccharomyces cerevisiae
?
inosine tetraphosphate + H2O
-
209852
Saccharomyces cerevisiae
ITP + phosphate
-
209852
Saccharomyces cerevisiae
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 45000, SDS-PAGE
Saccharomyces cerevisiae
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
40
-
adenosine-5'-pentaphosphate
-
Saccharomyces cerevisiae
723
-
adenosine 5'-tetraphosphate
-
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.8
-
-
Saccharomyces cerevisiae
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
3.8
7.2
50% of maximal activity at pH 3.8 and 7.2
Saccharomyces cerevisiae
Other publictions for EC 3.6.1.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744647
Amici
Synthesis and degradation of ...
Homo sapiens
Cell Chem. Biol.
24
553-564
2017
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
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-
-
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-
1
-
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-
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-
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668579
Guranowski
Methylene analogues of adenosi ...
Homo sapiens, Lupinus luteus
FEBS J.
273
829-838
2006
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209852
Guranowski
Adenosine 5'-tetraphosphate ph ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1380
232-238
1998
-
-
-
-
-
-
3
1
-
4
1
-
-
3
-
-
-
-
-
-
-
-
4
1
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
4
1
-
-
-
-
-
-
-
-
-
4
1
-
-
-
2
1
1
-
-
-
-
-
-
-
-
209873
Guranowski
Adenosine 5'-tetraphosphate ph ...
Lupinus luteus
Biochem. J.
328
257-262
1997
-
-
-
-
-
-
3
1
-
4
1
-
-
1
-
-
1
-
-
1
1
1
5
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
4
1
-
-
-
-
1
-
1
1
1
5
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
209871
Small
Purification and properties of ...
Oryctolagus cuniculus
Biochemistry
5
14-26
1966
-
-
-
-
-
-
3
2
-
5
-
-
-
1
-
-
1
-
-
4
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
-
5
-
-
-
-
-
1
-
4
1
-
3
-
-
-
-
-
-
-
-
-
-
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