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Literature summary for 3.6.1.13 extracted from

  • Ito, R.; Sekiguchi, M.; Setoyama, D.; Nakatsu, Y.; Yamagata, Y.; Hayakawa, H.
    Cleavage of oxidized guanine nucleotide and ADP sugar by human NUDT5 protein (2011), J. Biochem., 149, 731-738.
    View publication on PubMed
Search for more literature for 3.6.1.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
8-oxo-dGDP the ADP-ribose cleavage is competitively inhibited by 8-oxo-dGDP (68% relative cleavage efficiency at 0.01 mM) Homo sapiens
8-oxo-dGMP the ADP-ribose cleavage is competitively inhibited by 8-oxo-dGMP (89% relative cleavage efficiency at 0.01 mM) Homo sapiens
ADP-ribose the 8-oxo-dGDP cleavage is competitively inhibited by ADP-ribose (22% relative cleavage efficiency at 0.005 mM) Homo sapiens
AMP the 8-oxo-dGDP cleavage is competitively inhibited by AMP (30% relative cleavage efficiency at 0.005 mM) Homo sapiens
additional information no inhibitory effect is observed with 8-oxo-dGMP Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0019
-
 ADP-ribose at pH 8.0 and 37°C Homo sapiens
0.0035
-
 8-oxo-dGDP at pH 10.0 and 37°C Homo sapiens
0.0038
-
 8-oxo-dGDP at pH 8.0 and 37°C Homo sapiens
0.036
-
 ADP-ribose at pH 10.0 and 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UKK9
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon resin column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-oxo-dGDP + H2O
-
 Homo sapiens 8-oxo-dGMP + phosphate
-
 ?
8-oxo-dGTP + H2O the enzyme hardly acts on 8-oxo-dGTP Homo sapiens ?
-
 ?
ADP-ribose + H2O ADP-ribose is a better substrate compared with 8-oxo-dGDP Homo sapiens AMP + ribose 5-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
NUDT5
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9 for cleavage of ADP-ribose Homo sapiens
10
-
 for cleavage of 8-oxo-dGDP Homo sapiens