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Literature summary for 3.6.1.13 extracted from

  • Yu, H.N.; Song, E.K.; Yoo, S.M.; Lee, Y.R.; Han, M.K.; Yim, C.Y.; Kwak, J.Y.; Kim, J.S.
    Activation of NUDT5, an ADP-ribose pyrophosphatase, by nitric oxide-mediated ADP-ribosylation (2007), Biochem. Biophys. Res. Commun., 354, 764-768.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
nitric oxide NO stimulates non-enzymatic ADP-ribosylation, at cysteine residues in the presence of reductant, of NUDT5 using ADP-ribose and consequently activates its ADPRase activity. ADPRase activity in J774 macrophage cells is increased by the treatment with SNP, an exogenous NO generator, or TNF-alpha/IFN-gamma, endogenous NO inducers. NO has a regulatory role, overview Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene NUDT5, expression in murine J774A.1 macrophage cells, expression of His6-tagged NUDT5 in Escherichia coli strain BL21 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP-ribose + H2O Homo sapiens
-
AMP + D-ribose 5-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
gene NUDT5
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged NUDT5 from Escherichia coli strain BL21 by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,Nepsilon-etheno-ADP-ribose + H2O fluorogenic substrate Homo sapiens 1,Nepsilon-etheno-AMP-ribose + phosphate the product is converted to fluorescent 1,Nepsilon-etheno-adenosine by alkaline phosphatase for detection ?
ADP-ribose + H2O
-
Homo sapiens AMP + D-ribose 5-phosphate
-
?

Synonyms

Synonyms Comment Organism
ADP-ribose pyrophosphatase
-
Homo sapiens
ADPR pyrophosphatase
-
Homo sapiens
ADPRase
-
Homo sapiens
ADPRase-I
-
Homo sapiens
More the enzyme is a member of a superfamily of Nudix hydrolases Homo sapiens
NUDT5
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens