Crystallization (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
E108Q | kcat/Km is 9.6fold lower than wild-type value | Thermus thermophilus |
E63Q | kcat/Km is 173fold lower than wild-type value | Thermus thermophilus |
E70Q | kcat/Km is 8.9fold lower than wild-type value | Thermus thermophilus |
E73Q | kcat/Km is 1.8fold lower than wild-type value | Thermus thermophilus |
E82Q | kcat/Km is 118171fold lower than wild-type value | Thermus thermophilus |
E85Q | kcat/Km is 3.8fold lower than wild-type value | Thermus thermophilus |
E86Q | kcat/Km is 6722fold lower than wild-type value | Thermus thermophilus |
H33A | kcat/Km is 10fold lower than wild-type value | Thermus thermophilus |
I19A | kcat/Km is 22fold lower than wild-type value | Thermus thermophilus |
L68A | kcat/Km is 5.3fold lower than wild-type value | Thermus thermophilus |
Q52A | kcat/Km is 3.5fold lower than wild-type value | Thermus thermophilus |
R18Q | kcat/Km is 1.5fold higher than wild-type value | Thermus thermophilus |
R27Q | kcat/Km is 1.04fold lower than wild-type value | Thermus thermophilus |
R54Q | kcat/Km is 1589fold lower than wild-type value | Thermus thermophilus |
R81Q | kcat/Km is 77fold lower than wild-type value | Thermus thermophilus |
S102A | kcat/Km is 28.3fold lower than wild-type value | Thermus thermophilus |
S153A | kcat/Km is 2.9fold lower than wild-type value | Thermus thermophilus |
T110A | kcat/Km is 2.3fold lower than wild-type value | Thermus thermophilus |
T155A | kcat/Km is 1.9fold lower than wild-type value | Thermus thermophilus |
Y28Q | kcat/Km is 4fold lower than wild-type value | Thermus thermophilus |
Y99F | kcat/Km is 2.6fold lower than wild-type value | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fluoride | mixed inhibition mechanism | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme E70Q | Thermus thermophilus | |
0.027 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme R81Q | Thermus thermophilus | |
0.036 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme E73Q | Thermus thermophilus | |
0.043 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme E85Q | Thermus thermophilus | |
0.05 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme T155A | Thermus thermophilus | |
0.06 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme T110A | Thermus thermophilus | |
0.09 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme Y99F | Thermus thermophilus | |
0.1 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme E86Q | Thermus thermophilus | |
0.1 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme S153A | Thermus thermophilus | |
0.11 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme E82Q | Thermus thermophilus | |
0.11 | - |
ADP-ribose | 25°C, pH 7.6, wild-type enzyme | Thermus thermophilus | |
0.12 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme R18Q | Thermus thermophilus | |
0.14 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme R27Q | Thermus thermophilus | |
0.24 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme Q52A | Thermus thermophilus | |
0.29 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme E108Q | Thermus thermophilus | |
0.31 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme L68A | Thermus thermophilus | |
0.4 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme E63Q | Thermus thermophilus | |
0.4 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme Y28Q | Thermus thermophilus | |
0.42 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme H33A | Thermus thermophilus | |
0.65 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme R54Q | Thermus thermophilus | |
0.69 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme S102A | Thermus thermophilus | |
1.1 | - |
GDP-ribose | 25°C, pH 7.6, wild-type enzyme | Thermus thermophilus | |
1.24 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme I19A | Thermus thermophilus | |
1.36 | - |
CDP-ribose | 25°C, pH 7.6, wild-type enzyme | Thermus thermophilus | |
3.16 | - |
ADP-glucose | 25°C, pH 7.6, wild-type enzyme | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + H2O | kcat/Km is 3% of the kcat/Km for ADP-ribose | Thermus thermophilus | AMP + alpha-D-glucose 1-phosphate | - |
? | |
ADP-ribose + H2O | interactions responsible for the substrate recognition are located at the terminal moieties of the substrate. The adenine moiety is recognized by Ile-19 and the main chain carbonyl group of Glu-29 and/or Gly-104. The terminal ribose moiety is recognized by the sum of some weak interactions with multiple residues that are close in space. Glu-82 and Glu-86 are essential for catalysis but unlikely to act as a catalytic base. Two-metal ion mechanism for the catalysis of ADPRase in which a water molecule is activated to act as a nucleophile by the cations coordinated by Glu-82 and Glu-86. Arg-54, Glu-70, Arg-81, and Glu-85 are predicted to support this nucleophilic attack on the R-phosphate of the substrate | Thermus thermophilus | AMP + D-ribose 5-phosphate | - |
? | |
CDP-ribose + H2O | kcat/Km is 2.5% of the kcat/Km for ADP-ribose | Thermus thermophilus | CMP + D-ribose 5-phosphate | - |
? | |
GDP-ribose + H2O | kcat/Km is 3% of the kcat/Km for ADP-ribose | Thermus thermophilus | GMP + D-ribose 5-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADP-ribose pyrophosphatase | - |
Thermus thermophilus |
ADPRase | - |
Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme I19A | Thermus thermophilus | |
0.000939 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme E82Q | Thermus thermophilus | |
0.00153 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme E86Q | Thermus thermophilus | |
0.035 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme R81Q | Thermus thermophilus | |
0.0411 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme R54Q | Thermus thermophilus | |
0.052 | 2.1 | ADP-ribose | 25°C, pH 7.6, mutant enzyme Y28Q | Thermus thermophilus | |
0.057 | 0.65 | ADP-ribose | 25°C, pH 7.6, wild-type enzyme | Thermus thermophilus | |
0.23 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme E63Q | Thermus thermophilus | |
0.41 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme E70Q | Thermus thermophilus | |
0.97 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme R18Q | Thermus thermophilus | |
1.12 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme E85Q | Thermus thermophilus | |
2.07 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme E73Q | Thermus thermophilus | |
2.42 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme T155A | Thermus thermophilus | |
2.43 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme S102A | Thermus thermophilus | |
2.65 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme T110A | Thermus thermophilus | |
2.97 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme E108Q | Thermus thermophilus | |
3.3 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme S153A | Thermus thermophilus | |
3.32 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme Y99F | Thermus thermophilus | |
3.36 | - |
CDP-ribose | 25°C, pH 7.6, wild-type enzyme | Thermus thermophilus | |
3.57 | - |
GDP-ribose | 25°C, pH 7.6, wild-type enzyme | Thermus thermophilus | |
4.16 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme H33A | Thermus thermophilus | |
5.68 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme I19A | Thermus thermophilus | |
5.93 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme L68A | Thermus thermophilus | |
6.74 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme Q52A | Thermus thermophilus | |
9.1 | - |
ADP-glucose | 25°C, pH 7.6, wild-type enzyme | Thermus thermophilus | |
10.04 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme Y28Q | Thermus thermophilus | |
10.65 | - |
ADP-ribose | 25°C, pH 7.6, wild-type enzyme | Thermus thermophilus | |
12.9 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme R27Q | Thermus thermophilus | |
17.05 | - |
ADP-ribose | 25°C, pH 7.6, mutant enzyme R18Q | Thermus thermophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
fluoride | 25°C, pH 7.6, Ki(2), Ki(1) is 0.15 mM | Thermus thermophilus | |
0.15 | - |
fluoride | 25°C, pH 7.6, Ki(1), Ki(2) is 0.015 mM | Thermus thermophilus |