-
The NTP pyrophosphatase DCTPP1 contributes to the homoeostasis and cleansing of the dNTP pool in human cells
Requena, C.E.; Perez-Moreno, G.; Ruiz-Perez, L.M.; Vidal, A.E.; Gonzalez-Pacanowska, D.; Biochem. J. 459, 171-180 (2014) 
Data extracted from this reference:
Cloned(Commentary)
Cloned (Commentary)
Organism
Homo sapiens
Engineering
Protein Variants
Commentary
Organism
E63Q
catalytically inactive
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0092
-
5-formyl-dCTP
pH 8.0, 25°C
Homo sapiens
0.0168
-
5-iodo-dCTP
pH 8.0, 25°C
Homo sapiens
0.0333
-
5-Methyl-dCTP
pH 8.0, 25°C
Homo sapiens
0.0365
-
5-bromo-dCTP
pH 8.0, 25°C
Homo sapiens
0.0476
-
dCTP
pH 8.0, 25°C
Homo sapiens
0.0629
-
5-hydroxymethyl-dCTP
pH 8.0, 25°C
Homo sapiens
0.065
-
dTTP
pH 8.0, 25°C
Homo sapiens
0.0793
-
dATP
pH 8.0, 25°C
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20800
-
x * 20800, recombinant protein with His-tag, SDS-PAGE
Homo sapiens
Organism
Organism
UniProt
Commentary
Textmining
Homo sapiens
Q9H773
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
HeLa cell
-
Homo sapiens
-
MRC-5 cell
-
Homo sapiens
-
TK-6 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5-bromo-dCTP + H2O
-
733297
Homo sapiens
5-bromo-dCMP + diphosphate
-
-
-
?
5-formyl-dCTP + H2O
-
733297
Homo sapiens
5-formyl-dCMP + diphosphate
-
-
-
?
5-hydroxymethyl-dCTP + H2O
-
733297
Homo sapiens
5-hydroxymethyl-dCMP + diphosphate
-
-
-
?
5-iodo-dCTP + H2O
-
733297
Homo sapiens
5-iodo-dCMP + diphosphate
-
-
-
?
5-methyl-dCTP + H2O
-
733297
Homo sapiens
5-methyl-dCMP + diphosphate
-
-
-
?
dATP + H2O
-
733297
Homo sapiens
dAMP + diphosphate
-
-
-
?
dCTP + H2O
-
733297
Homo sapiens
dCMP + diphosphate
-
-
-
?
dTTP + H2O
-
733297
Homo sapiens
dTMP + diphosphate
-
-
-
?
additional information
no substrtaes: ATP, CTP, UTP, dCDP, 5-methyl-dCDP, dGTP. Poor substrate: dUTP
733297
Homo sapiens
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 20800, recombinant protein with His-tag, SDS-PAGE
Homo sapiens
Synonyms
Synonyms
Commentary
Organism
XTP3-TPA
-
Homo sapiens
XTP3-transactivated protein A
-
Homo sapiens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.8
-
dTTP
pH 8.0, 25°C
Homo sapiens
2
-
dATP
pH 8.0, 25°C
Homo sapiens
3
-
5-formyl-dCTP
pH 8.0, 25°C
Homo sapiens
4.8
-
5-Methyl-dCTP
pH 8.0, 25°C
Homo sapiens
5.6
-
5-hydroxymethyl-dCTP
pH 8.0, 25°C
Homo sapiens
5.7
-
dCTP
pH 8.0, 25°C
Homo sapiens
6.2
-
5-iodo-dCTP
pH 8.0, 25°C
Homo sapiens
9.3
-
5-bromo-dCTP
pH 8.0, 25°C
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Engineering (protein specific)
Protein Variants
Commentary
Organism
E63Q
catalytically inactive
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0092
-
5-formyl-dCTP
pH 8.0, 25°C
Homo sapiens
0.0168
-
5-iodo-dCTP
pH 8.0, 25°C
Homo sapiens
0.0333
-
5-Methyl-dCTP
pH 8.0, 25°C
Homo sapiens
0.0365
-
5-bromo-dCTP
pH 8.0, 25°C
Homo sapiens
0.0476
-
dCTP
pH 8.0, 25°C
Homo sapiens
0.0629
-
5-hydroxymethyl-dCTP
pH 8.0, 25°C
Homo sapiens
0.065
-
dTTP
pH 8.0, 25°C
Homo sapiens
0.0793
-
dATP
pH 8.0, 25°C
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20800
-
x * 20800, recombinant protein with His-tag, SDS-PAGE
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
HeLa cell
-
Homo sapiens
-
MRC-5 cell
-
Homo sapiens
-
TK-6 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5-bromo-dCTP + H2O
-
733297
Homo sapiens
5-bromo-dCMP + diphosphate
-
-
-
?
5-formyl-dCTP + H2O
-
733297
Homo sapiens
5-formyl-dCMP + diphosphate
-
-
-
?
5-hydroxymethyl-dCTP + H2O
-
733297
Homo sapiens
5-hydroxymethyl-dCMP + diphosphate
-
-
-
?
5-iodo-dCTP + H2O
-
733297
Homo sapiens
5-iodo-dCMP + diphosphate
-
-
-
?
5-methyl-dCTP + H2O
-
733297
Homo sapiens
5-methyl-dCMP + diphosphate
-
-
-
?
dATP + H2O
-
733297
Homo sapiens
dAMP + diphosphate
-
-
-
?
dCTP + H2O
-
733297
Homo sapiens
dCMP + diphosphate
-
-
-
?
dTTP + H2O
-
733297
Homo sapiens
dTMP + diphosphate
-
-
-
?
additional information
no substrtaes: ATP, CTP, UTP, dCDP, 5-methyl-dCDP, dGTP. Poor substrate: dUTP
733297
Homo sapiens
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 20800, recombinant protein with His-tag, SDS-PAGE
Homo sapiens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.8
-
dTTP
pH 8.0, 25°C
Homo sapiens
2
-
dATP
pH 8.0, 25°C
Homo sapiens
3
-
5-formyl-dCTP
pH 8.0, 25°C
Homo sapiens
4.8
-
5-Methyl-dCTP
pH 8.0, 25°C
Homo sapiens
5.6
-
5-hydroxymethyl-dCTP
pH 8.0, 25°C
Homo sapiens
5.7
-
dCTP
pH 8.0, 25°C
Homo sapiens
6.2
-
5-iodo-dCTP
pH 8.0, 25°C
Homo sapiens
9.3
-
5-bromo-dCTP
pH 8.0, 25°C
Homo sapiens
General Information
General Information
Commentary
Organism
physiological function
DCTPP1-deficient cells accumulate high levels of dCTP and are hypersensitive to exposure to the nucleoside analogues 5-iodo-2'-deoxycytidine and 5-methyl-2'-deoxycytidine
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
physiological function
DCTPP1-deficient cells accumulate high levels of dCTP and are hypersensitive to exposure to the nucleoside analogues 5-iodo-2'-deoxycytidine and 5-methyl-2'-deoxycytidine
Homo sapiens
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
25
-
dATP
pH 8.0, 25°C
Homo sapiens
28
-
dTTP
pH 8.0, 25°C
Homo sapiens
90
-
5-hydroxymethyl-dCTP
pH 8.0, 25°C
Homo sapiens
119
-
dCTP
pH 8.0, 25°C
Homo sapiens
143
-
5-Methyl-dCTP
pH 8.0, 25°C
Homo sapiens
256
-
5-bromo-dCTP
pH 8.0, 25°C
Homo sapiens
322
-
5-formyl-dCTP
pH 8.0, 25°C
Homo sapiens
371
-
5-iodo-dCTP
pH 8.0, 25°C
Homo sapiens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
25
-
dATP
pH 8.0, 25°C
Homo sapiens
28
-
dTTP
pH 8.0, 25°C
Homo sapiens
90
-
5-hydroxymethyl-dCTP
pH 8.0, 25°C
Homo sapiens
119
-
dCTP
pH 8.0, 25°C
Homo sapiens
143
-
5-Methyl-dCTP
pH 8.0, 25°C
Homo sapiens
256
-
5-bromo-dCTP
pH 8.0, 25°C
Homo sapiens
322
-
5-formyl-dCTP
pH 8.0, 25°C
Homo sapiens
371
-
5-iodo-dCTP
pH 8.0, 25°C
Homo sapiens
Other publictions for EC 3.6.1.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
755792
Friese
Chemical genetics reveals a r ...
Homo sapiens
Angew. Chem. Int. Ed. Engl.
58
13009-13013
2019
-
-
1
-
1
-
5
2
-
-
-
2
-
1
-
-
1
-
-
1
-
-
2
-
2
-
-
-
-
-
-
-
-
1
-
4
-
-
1
-
-
1
-
4
5
1
2
-
-
-
2
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
756417
Martinez-Arribas
DCTPP1 prevents a mutator phe ...
Homo sapiens
Cell. Mol. Life Sci.
77
1645-1660
2019
-
1
-
-
1
-
-
-
3
-
-
2
-
3
-
-
-
-
-
1
-
-
2
-
5
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
3
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
756185
Llona-Minguez
Diverse heterocyclic scaffold ...
Homo sapiens
Bioorg. Med. Chem. Lett.
27
3219-3225
2017
-
-
-
-
-
-
51
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
17
-
-
-
-
-
-
-
17
53
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
756187
Llona-Minguez
Diverse heterocyclic scaffold ...
Homo sapiens
Bioorg. Med. Chem. Lett.
27
3897-3904
2017
-
-
-
-
-
-
69
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
42
-
-
-
-
-
-
-
42
69
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
757389
Llona-Minguez
Identification of triazolothi ...
Homo sapiens
J. Med. Chem.
60
2148-2154
2017
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
757391
Llona-Minguez
Piperazin-1-ylpyridazine deri ...
Homo sapiens
J. Med. Chem.
60
4279-4292
2017
-
1
-
-
-
-
2
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
755999
Guiomar
The nucleotidohydrolases DCTP ...
Homo sapiens
Biochem. J.
473
2635-2643
2016
-
1
1
-
1
-
-
1
-
1
-
1
-
2
-
-
-
-
-
1
-
-
2
-
4
2
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
1
-
1
-
1
-
-
-
-
-
1
-
-
2
-
2
-
-
-
-
-
-
-
2
6
6
2
-
-
757386
Llona-Minguez
Discovery of the first potent ...
Homo sapiens
J. Med. Chem.
59
1140-1148
2016
-
-
-
-
-
-
37
-
-
-
-
-
-
6
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
32
-
-
-
-
-
-
-
32
37
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
734816
Song
Human dCTP pyrophosphatase 1 p ...
Homo sapiens
Oncogenesis
4
e159
2015
-
-
-
1
-
-
-
7
-
-
-
-
-
4
-
-
-
-
-
3
-
-
8
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
3
-
-
8
-
-
-
-
7
-
1
-
-
-
1
1
-
7
7
733297
Requena
The NTP pyrophosphatase DCTPP1 ...
Homo sapiens
Biochem. J.
459
171-180
2014
-
-
1
-
1
-
-
8
-
-
1
-
-
6
-
-
1
-
-
3
-
-
9
1
2
-
-
-
8
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
8
-
-
1
-
-
-
-
1
-
3
-
-
9
1
-
-
-
8
-
-
-
-
-
1
1
-
8
8
733777
Zhang
dCTP pyrophosphohydrase exhibi ...
Homo sapiens
Eur. J. Histochem.
57
e29
2013
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719287
Corson
Triptolide directly inhibits d ...
Homo sapiens
ChemBioChem
12
1767-1773
2011
-
-
1
-
-
-
5
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
2
1
2
-
-
-
1
-
-
-
-
4
-
-
-
-
1
-
-
-
-
-
5
4
1
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
1
-
-
-
-
1
-
-
1
1
1
722230
Nonaka
Mouse RS21-C6 is a mammalian 2 ...
Mus musculus
FEBS J.
276
1654-1666
2009
-
-
1
-
-
-
1
13
1
3
2
-
-
3
-
-
-
-
-
3
-
-
14
1
4
1
-
-
13
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
13
1
3
2
-
-
-
-
-
-
3
-
-
14
1
1
-
-
13
1
-
-
-
-
-
-
-
13
13
209857
Mosig
Several new bacteriophage t4 g ...
Escherichia virus T4
Gene
223
143-155
1998
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209858
Gary
A species barrier between bact ...
Enterobacteria phage T2, Escherichia virus T4
Genetics
148
1461-1473
1998
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2
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9
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209853
Neale
Enzymes of pyrimidine deoxyrib ...
Mycoplasma mycoides
J. Bacteriol.
156
1001-1005
1983
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5
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209854
Allen
T4 phage deoxyribonucleotide-s ...
Escherichia virus T4
J. Biol. Chem.
258
5746-5753
1983
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1
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209855
Chiu
Characteristics of a bacteriop ...
Escherichia virus T4
J. Biol. Chem.
257
15087-15097
1982
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209856
Zimmerman
Deoxycytidine di- and triphosp ...
Enterobacteria phage T2
J. Biol. Chem.
236
1480-1486
1961
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