BRENDA - Enzyme Database show
show all sequences of 3.6.1.12

Mouse RS21-C6 is a mammalian 2'-deoxycytidine 5'-triphosphate pyrophosphohydrolase that prefers 5-iodocytosine

Nonaka, M.; Tsuchimoto, D.; Sakumi, K.; Nakabeppu, Y.; FEBS J. 276, 1654-1666 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Mus musculus
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
not inhibitory: ITP up to 0.5 mM
Mus musculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0039
-
5-iodo-dCTP
pH 8.0, 30°C
Mus musculus
0.0217
-
5-bromo-dCTP
pH 8.0, 30°C
Mus musculus
0.034
-
5-fluoro-dUTP
pH 8.0, 30°C
Mus musculus
0.0441
-
dCTP
pH 8.0, 30°C
Mus musculus
0.0485
-
5-Methyl-dCTP
pH 8.0, 30°C
Mus musculus
0.05
-
5-chloro-dCTP
pH 8.0, 30°C
Mus musculus
0.107
-
2-chloro-dATP
pH 8.0, 30°C
Mus musculus
0.118
-
dATP
pH 8.0, 30°C
Mus musculus
0.164
-
5-hydroxy-dCTP
pH 8.0, 30°C
Mus musculus
0.169
-
2-hydroxy-dATP
pH 8.0, 30°C
Mus musculus
0.407
-
dTTP
pH 8.0, 30°C
Mus musculus
0.484
-
dUTP
pH 8.0, 30°C
Mus musculus
0.529
-
CTP
pH 8.0, 30°C
Mus musculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
exclusivelyx located in the cytosol
Mus musculus
5829
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
MgCl2
maximum activity in presence of 100 mM MgCl2
Mus musculus
MnCl2
up to 40% of the activity with MgCl2
Mus musculus
additional information
divalent cation is absolutely required
Mus musculus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18783
-
x * 19000, SDS-PAGE, x * 18783, calculated
Mus musculus
19000
-
x * 19000, SDS-PAGE, x * 18783, calculated
Mus musculus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
Q9QY93
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
heart
enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland
Mus musculus
-
liver
enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland
Mus musculus
-
salivary gland
enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-chloro-dATP + H2O
-
722230
Mus musculus
2-chloro-AMP + diphosphate
-
-
-
?
2-hydroxy-dATP + H2O
-
722230
Mus musculus
2-hydroxy-AMP + diphosphate
-
-
-
?
5-bromo-dCTP + H2O
-
722230
Mus musculus
5-bromo-dCMP + diphosphate
-
-
-
?
5-chloro-dCTP + H2O
-
722230
Mus musculus
5-chloro-dCMP + diphosphate
-
-
-
?
5-fluoro-dUTP + H2O
-
722230
Mus musculus
5-fluoro-dUMP + diphosphate
-
-
-
?
5-hydroxy-dCTP + H2O
-
722230
Mus musculus
5-hydroxy-dCMP + diphosphate
-
-
-
?
5-iodo-dCTP + H2O
-
722230
Mus musculus
5-iodo-dCMP + diphosphate
-
-
-
?
5-methyl-dCTP + H2O
-
722230
Mus musculus
5-methyl-dCMP + diphosphate
-
-
-
?
CTP + H2O
-
722230
Mus musculus
CMP + diphosphate
-
-
-
?
dATP + H2O
-
722230
Mus musculus
dAMP + diphosphate
-
-
-
?
dCTP + H2O
-
722230
Mus musculus
dCMP + diphosphate
-
-
-
?
dTTP + H2O
-
722230
Mus musculus
dTMP + diphosphate
-
-
-
?
dUTP + H2O
-
722230
Mus musculus
dUMP + diphosphate
-
-
-
?
additional information
no substrates: ATP, GTP, UTP, ITP, 2-hydroxy-ATP, dGTP, dITP, 8-oxo-dGTP, dCDP, dADP
722230
Mus musculus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 19000, SDS-PAGE, x * 18783, calculated
Mus musculus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
temperature-dependent increase of activity up to 60°C
Mus musculus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.31
-
CTP
pH 8.0, 30°C
Mus musculus
0.53
-
dATP
pH 8.0, 30°C
Mus musculus
0.58
-
5-fluoro-dUTP
pH 8.0, 30°C
Mus musculus
0.76
-
dTTP
pH 8.0, 30°C
Mus musculus
0.76
-
dUTP
pH 8.0, 30°C
Mus musculus
0.87
-
5-hydroxy-dCTP
pH 8.0, 30°C
Mus musculus
0.94
-
5-iodo-dCTP
pH 8.0, 30°C
Mus musculus
1.33
-
5-Methyl-dCTP
pH 8.0, 30°C
Mus musculus
1.37
-
dCTP
pH 8.0, 30°C
Mus musculus
1.39
-
2-hydroxy-dATP
pH 8.0, 30°C
Mus musculus
1.49
-
5-bromo-dCTP
pH 8.0, 30°C
Mus musculus
1.5
-
5-chloro-dCTP
pH 8.0, 30°C
Mus musculus
1.57
-
2-chloro-dATP
pH 8.0, 30°C
Mus musculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Mus musculus
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Mus musculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
not inhibitory: ITP up to 0.5 mM
Mus musculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0039
-
5-iodo-dCTP
pH 8.0, 30°C
Mus musculus
0.0217
-
5-bromo-dCTP
pH 8.0, 30°C
Mus musculus
0.034
-
5-fluoro-dUTP
pH 8.0, 30°C
Mus musculus
0.0441
-
dCTP
pH 8.0, 30°C
Mus musculus
0.0485
-
5-Methyl-dCTP
pH 8.0, 30°C
Mus musculus
0.05
-
5-chloro-dCTP
pH 8.0, 30°C
Mus musculus
0.107
-
2-chloro-dATP
pH 8.0, 30°C
Mus musculus
0.118
-
dATP
pH 8.0, 30°C
Mus musculus
0.164
-
5-hydroxy-dCTP
pH 8.0, 30°C
Mus musculus
0.169
-
2-hydroxy-dATP
pH 8.0, 30°C
Mus musculus
0.407
-
dTTP
pH 8.0, 30°C
Mus musculus
0.484
-
dUTP
pH 8.0, 30°C
Mus musculus
0.529
-
CTP
pH 8.0, 30°C
Mus musculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
exclusivelyx located in the cytosol
Mus musculus
5829
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
MgCl2
maximum activity in presence of 100 mM MgCl2
Mus musculus
MnCl2
up to 40% of the activity with MgCl2
Mus musculus
additional information
divalent cation is absolutely required
Mus musculus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18783
-
x * 19000, SDS-PAGE, x * 18783, calculated
Mus musculus
19000
-
x * 19000, SDS-PAGE, x * 18783, calculated
Mus musculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
heart
enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland
Mus musculus
-
liver
enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland
Mus musculus
-
salivary gland
enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-chloro-dATP + H2O
-
722230
Mus musculus
2-chloro-AMP + diphosphate
-
-
-
?
2-hydroxy-dATP + H2O
-
722230
Mus musculus
2-hydroxy-AMP + diphosphate
-
-
-
?
5-bromo-dCTP + H2O
-
722230
Mus musculus
5-bromo-dCMP + diphosphate
-
-
-
?
5-chloro-dCTP + H2O
-
722230
Mus musculus
5-chloro-dCMP + diphosphate
-
-
-
?
5-fluoro-dUTP + H2O
-
722230
Mus musculus
5-fluoro-dUMP + diphosphate
-
-
-
?
5-hydroxy-dCTP + H2O
-
722230
Mus musculus
5-hydroxy-dCMP + diphosphate
-
-
-
?
5-iodo-dCTP + H2O
-
722230
Mus musculus
5-iodo-dCMP + diphosphate
-
-
-
?
5-methyl-dCTP + H2O
-
722230
Mus musculus
5-methyl-dCMP + diphosphate
-
-
-
?
CTP + H2O
-
722230
Mus musculus
CMP + diphosphate
-
-
-
?
dATP + H2O
-
722230
Mus musculus
dAMP + diphosphate
-
-
-
?
dCTP + H2O
-
722230
Mus musculus
dCMP + diphosphate
-
-
-
?
dTTP + H2O
-
722230
Mus musculus
dTMP + diphosphate
-
-
-
?
dUTP + H2O
-
722230
Mus musculus
dUMP + diphosphate
-
-
-
?
additional information
no substrates: ATP, GTP, UTP, ITP, 2-hydroxy-ATP, dGTP, dITP, 8-oxo-dGTP, dCDP, dADP
722230
Mus musculus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 19000, SDS-PAGE, x * 18783, calculated
Mus musculus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
temperature-dependent increase of activity up to 60°C
Mus musculus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.31
-
CTP
pH 8.0, 30°C
Mus musculus
0.53
-
dATP
pH 8.0, 30°C
Mus musculus
0.58
-
5-fluoro-dUTP
pH 8.0, 30°C
Mus musculus
0.76
-
dTTP
pH 8.0, 30°C
Mus musculus
0.76
-
dUTP
pH 8.0, 30°C
Mus musculus
0.87
-
5-hydroxy-dCTP
pH 8.0, 30°C
Mus musculus
0.94
-
5-iodo-dCTP
pH 8.0, 30°C
Mus musculus
1.33
-
5-Methyl-dCTP
pH 8.0, 30°C
Mus musculus
1.37
-
dCTP
pH 8.0, 30°C
Mus musculus
1.39
-
2-hydroxy-dATP
pH 8.0, 30°C
Mus musculus
1.49
-
5-bromo-dCTP
pH 8.0, 30°C
Mus musculus
1.5
-
5-chloro-dCTP
pH 8.0, 30°C
Mus musculus
1.57
-
2-chloro-dATP
pH 8.0, 30°C
Mus musculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Mus musculus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.156
-
CTP
pH 8.0, 30°C
Mus musculus
1.57
-
dUTP
pH 8.0, 30°C
Mus musculus
1.87
-
dTTP
pH 8.0, 30°C
Mus musculus
1.9
-
5-fluoro-dUTP
pH 8.0, 30°C
Mus musculus
4.49
-
dATP
pH 8.0, 30°C
Mus musculus
5.3
-
5-hydroxy-dCTP
pH 8.0, 30°C
Mus musculus
8.22
-
2-hydroxy-dATP
pH 8.0, 30°C
Mus musculus
14.7
-
2-chloro-dATP
pH 8.0, 30°C
Mus musculus
27.4
-
5-Methyl-dCTP
pH 8.0, 30°C
Mus musculus
30
-
5-chloro-dCTP
pH 8.0, 30°C
Mus musculus
31.1
-
dCTP
pH 8.0, 30°C
Mus musculus
68.7
-
5-bromo-dCTP
pH 8.0, 30°C
Mus musculus
241
-
5-iodo-dCTP
pH 8.0, 30°C
Mus musculus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.156
-
CTP
pH 8.0, 30°C
Mus musculus
1.57
-
dUTP
pH 8.0, 30°C
Mus musculus
1.87
-
dTTP
pH 8.0, 30°C
Mus musculus
1.9
-
5-fluoro-dUTP
pH 8.0, 30°C
Mus musculus
4.49
-
dATP
pH 8.0, 30°C
Mus musculus
5.3
-
5-hydroxy-dCTP
pH 8.0, 30°C
Mus musculus
8.22
-
2-hydroxy-dATP
pH 8.0, 30°C
Mus musculus
14.7
-
2-chloro-dATP
pH 8.0, 30°C
Mus musculus
27.4
-
5-Methyl-dCTP
pH 8.0, 30°C
Mus musculus
30
-
5-chloro-dCTP
pH 8.0, 30°C
Mus musculus
31.1
-
dCTP
pH 8.0, 30°C
Mus musculus
68.7
-
5-bromo-dCTP
pH 8.0, 30°C
Mus musculus
241
-
5-iodo-dCTP
pH 8.0, 30°C
Mus musculus
Other publictions for EC 3.6.1.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734816
Song
Human dCTP pyrophosphatase 1 p ...
Homo sapiens
Oncogenesis
4
e159
2015
-
-
-
1
-
-
-
7
-
-
-
-
-
2
-
-
-
-
-
3
-
-
8
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
3
-
-
8
-
-
-
-
7
-
1
-
-
-
1
1
-
7
7
733297
Requena
The NTP pyrophosphatase DCTPP1 ...
Homo sapiens
Biochem. J.
459
171-180
2014
-
-
1
-
1
-
-
8
-
-
1
-
-
2
-
-
1
-
-
3
-
-
9
1
-
-
-
8
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
8
-
-
1
-
-
-
-
1
-
3
-
-
9
1
-
-
-
8
-
-
-
-
-
1
1
-
8
8
733777
Zhang
dCTP pyrophosphohydrase exhibi ...
Homo sapiens
Eur. J. Histochem.
57
e29
2013
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719287
Corson
Triptolide directly inhibits d ...
Homo sapiens
ChemBioChem
12
1767-1773
2011
-
-
1
-
-
-
5
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
1
-
-
-
-
4
-
-
-
-
1
-
-
-
-
-
5
4
1
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
1
-
-
-
-
1
-
-
1
1
1
722230
Nonaka
Mouse RS21-C6 is a mammalian 2 ...
Mus musculus
FEBS J.
276
1654-1666
2009
-
-
1
-
-
-
1
13
1
3
2
-
-
2
-
-
-
-
-
3
-
-
14
1
1
-
-
13
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
13
1
3
2
-
-
-
-
-
-
3
-
-
14
1
1
-
-
13
1
-
-
-
-
-
-
-
13
13
209857
Mosig
Several new bacteriophage t4 g ...
Escherichia virus T4
Gene
223
143-155
1998
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209858
Gary
A species barrier between bact ...
Enterobacteria phage T2, Escherichia virus T4
Genetics
148
1461-1473
1998
-
-
2
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209853
Neale
Enzymes of pyrimidine deoxyrib ...
Mycoplasma mycoides
J. Bacteriol.
156
1001-1005
1983
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
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209854
Allen
T4 phage deoxyribonucleotide-s ...
Escherichia virus T4
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5746-5753
1983
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1
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209855
Chiu
Characteristics of a bacteriop ...
Escherichia virus T4
J. Biol. Chem.
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15087-15097
1982
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209856
Zimmerman
Deoxycytidine di- and triphosp ...
Enterobacteria phage T2
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1480-1486
1961
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