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Literature summary for 3.6.1.12 extracted from

  • Nonaka, M.; Tsuchimoto, D.; Sakumi, K.; Nakabeppu, Y.
    Mouse RS21-C6 is a mammalian 2'-deoxycytidine 5'-triphosphate pyrophosphohydrolase that prefers 5-iodocytosine (2009), FEBS J., 276, 1654-1666.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: ITP up to 0.5 mM Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0039
-
5-iodo-dCTP pH 8.0, 30°C Mus musculus
0.0217
-
5-bromo-dCTP pH 8.0, 30°C Mus musculus
0.034
-
5-fluoro-dUTP pH 8.0, 30°C Mus musculus
0.0441
-
dCTP pH 8.0, 30°C Mus musculus
0.0485
-
5-Methyl-dCTP pH 8.0, 30°C Mus musculus
0.05
-
5-chloro-dCTP pH 8.0, 30°C Mus musculus
0.107
-
2-chloro-dATP pH 8.0, 30°C Mus musculus
0.118
-
dATP pH 8.0, 30°C Mus musculus
0.164
-
5-hydroxy-dCTP pH 8.0, 30°C Mus musculus
0.169
-
2-hydroxy-dATP pH 8.0, 30°C Mus musculus
0.407
-
dTTP pH 8.0, 30°C Mus musculus
0.484
-
dUTP pH 8.0, 30°C Mus musculus
0.529
-
CTP pH 8.0, 30°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol exclusivelyx located in the cytosol Mus musculus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2 maximum activity in presence of 100 mM MgCl2 Mus musculus
MnCl2 up to 40% of the activity with MgCl2 Mus musculus
additional information divalent cation is absolutely required Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18783
-
x * 19000, SDS-PAGE, x * 18783, calculated Mus musculus
19000
-
x * 19000, SDS-PAGE, x * 18783, calculated Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q9QY93
-
-

Source Tissue

Source Tissue Comment Organism Textmining
heart enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland Mus musculus
-
liver enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland Mus musculus
-
salivary gland enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro-dATP + H2O
-
Mus musculus 2-chloro-AMP + diphosphate
-
?
2-hydroxy-dATP + H2O
-
Mus musculus 2-hydroxy-dAMP + diphosphate
-
?
5-bromo-dCTP + H2O
-
Mus musculus 5-bromo-dCMP + diphosphate
-
?
5-chloro-dCTP + H2O
-
Mus musculus 5-chloro-dCMP + diphosphate
-
?
5-fluoro-dUTP + H2O
-
Mus musculus 5-fluoro-dUMP + diphosphate
-
?
5-hydroxy-dCTP + H2O
-
Mus musculus 5-hydroxy-dCMP + diphosphate
-
?
5-iodo-dCTP + H2O
-
Mus musculus 5-iodo-dCMP + diphosphate
-
?
5-methyl-dCTP + H2O
-
Mus musculus 5-methyl-dCMP + diphosphate
-
?
CTP + H2O
-
Mus musculus CMP + diphosphate
-
?
dATP + H2O
-
Mus musculus dAMP + diphosphate
-
?
dCTP + H2O
-
Mus musculus dCMP + diphosphate
-
?
dTTP + H2O
-
Mus musculus dTMP + diphosphate
-
?
dUTP + H2O
-
Mus musculus dUMP + diphosphate
-
?
additional information no substrates: ATP, GTP, UTP, ITP, 2-hydroxy-ATP, dGTP, dITP, 8-oxo-dGTP, dCDP, dADP Mus musculus ?
-
?

Subunits

Subunits Comment Organism
? x * 19000, SDS-PAGE, x * 18783, calculated Mus musculus

Synonyms

Synonyms Comment Organism
dCTP pyrophosphatase 1
-
Mus musculus
dCTPase 1
-
Mus musculus
DCTPP1
-
Mus musculus
RS21-C6
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
temperature-dependent increase of activity up to 60°C Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.31
-
CTP pH 8.0, 30°C Mus musculus
0.53
-
dATP pH 8.0, 30°C Mus musculus
0.58
-
5-fluoro-dUTP pH 8.0, 30°C Mus musculus
0.76
-
dTTP pH 8.0, 30°C Mus musculus
0.76
-
dUTP pH 8.0, 30°C Mus musculus
0.87
-
5-hydroxy-dCTP pH 8.0, 30°C Mus musculus
0.94
-
5-iodo-dCTP pH 8.0, 30°C Mus musculus
1.33
-
5-Methyl-dCTP pH 8.0, 30°C Mus musculus
1.37
-
dCTP pH 8.0, 30°C Mus musculus
1.39
-
2-hydroxy-dATP pH 8.0, 30°C Mus musculus
1.49
-
5-bromo-dCTP pH 8.0, 30°C Mus musculus
1.5
-
5-chloro-dCTP pH 8.0, 30°C Mus musculus
1.57
-
2-chloro-dATP pH 8.0, 30°C Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.156
-
CTP pH 8.0, 30°C Mus musculus
1.57
-
dUTP pH 8.0, 30°C Mus musculus
1.87
-
dTTP pH 8.0, 30°C Mus musculus
1.9
-
5-fluoro-dUTP pH 8.0, 30°C Mus musculus
4.49
-
dATP pH 8.0, 30°C Mus musculus
5.3
-
5-hydroxy-dCTP pH 8.0, 30°C Mus musculus
8.22
-
2-hydroxy-dATP pH 8.0, 30°C Mus musculus
14.7
-
2-chloro-dATP pH 8.0, 30°C Mus musculus
27.4
-
5-Methyl-dCTP pH 8.0, 30°C Mus musculus
30
-
5-chloro-dCTP pH 8.0, 30°C Mus musculus
31.1
-
dCTP pH 8.0, 30°C Mus musculus
68.7
-
5-bromo-dCTP pH 8.0, 30°C Mus musculus
241
-
5-iodo-dCTP pH 8.0, 30°C Mus musculus