Cloned (Comment) | Organism |
---|---|
- |
Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: ITP up to 0.5 mM | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
5-iodo-dCTP | pH 8.0, 30°C | Mus musculus | |
0.0217 | - |
5-bromo-dCTP | pH 8.0, 30°C | Mus musculus | |
0.034 | - |
5-fluoro-dUTP | pH 8.0, 30°C | Mus musculus | |
0.0441 | - |
dCTP | pH 8.0, 30°C | Mus musculus | |
0.0485 | - |
5-Methyl-dCTP | pH 8.0, 30°C | Mus musculus | |
0.05 | - |
5-chloro-dCTP | pH 8.0, 30°C | Mus musculus | |
0.107 | - |
2-chloro-dATP | pH 8.0, 30°C | Mus musculus | |
0.118 | - |
dATP | pH 8.0, 30°C | Mus musculus | |
0.164 | - |
5-hydroxy-dCTP | pH 8.0, 30°C | Mus musculus | |
0.169 | - |
2-hydroxy-dATP | pH 8.0, 30°C | Mus musculus | |
0.407 | - |
dTTP | pH 8.0, 30°C | Mus musculus | |
0.484 | - |
dUTP | pH 8.0, 30°C | Mus musculus | |
0.529 | - |
CTP | pH 8.0, 30°C | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | exclusivelyx located in the cytosol | Mus musculus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | maximum activity in presence of 100 mM MgCl2 | Mus musculus | |
MnCl2 | up to 40% of the activity with MgCl2 | Mus musculus | |
additional information | divalent cation is absolutely required | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18783 | - |
x * 19000, SDS-PAGE, x * 18783, calculated | Mus musculus |
19000 | - |
x * 19000, SDS-PAGE, x * 18783, calculated | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9QY93 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland | Mus musculus | - |
liver | enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland | Mus musculus | - |
salivary gland | enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloro-dATP + H2O | - |
Mus musculus | 2-chloro-AMP + diphosphate | - |
? | |
2-hydroxy-dATP + H2O | - |
Mus musculus | 2-hydroxy-dAMP + diphosphate | - |
? | |
5-bromo-dCTP + H2O | - |
Mus musculus | 5-bromo-dCMP + diphosphate | - |
? | |
5-chloro-dCTP + H2O | - |
Mus musculus | 5-chloro-dCMP + diphosphate | - |
? | |
5-fluoro-dUTP + H2O | - |
Mus musculus | 5-fluoro-dUMP + diphosphate | - |
? | |
5-hydroxy-dCTP + H2O | - |
Mus musculus | 5-hydroxy-dCMP + diphosphate | - |
? | |
5-iodo-dCTP + H2O | - |
Mus musculus | 5-iodo-dCMP + diphosphate | - |
? | |
5-methyl-dCTP + H2O | - |
Mus musculus | 5-methyl-dCMP + diphosphate | - |
? | |
CTP + H2O | - |
Mus musculus | CMP + diphosphate | - |
? | |
dATP + H2O | - |
Mus musculus | dAMP + diphosphate | - |
? | |
dCTP + H2O | - |
Mus musculus | dCMP + diphosphate | - |
? | |
dTTP + H2O | - |
Mus musculus | dTMP + diphosphate | - |
? | |
dUTP + H2O | - |
Mus musculus | dUMP + diphosphate | - |
? | |
additional information | no substrates: ATP, GTP, UTP, ITP, 2-hydroxy-ATP, dGTP, dITP, 8-oxo-dGTP, dCDP, dADP | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 19000, SDS-PAGE, x * 18783, calculated | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
dCTP pyrophosphatase 1 | - |
Mus musculus |
dCTPase 1 | - |
Mus musculus |
DCTPP1 | - |
Mus musculus |
RS21-C6 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
temperature-dependent increase of activity up to 60°C | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
CTP | pH 8.0, 30°C | Mus musculus | |
0.53 | - |
dATP | pH 8.0, 30°C | Mus musculus | |
0.58 | - |
5-fluoro-dUTP | pH 8.0, 30°C | Mus musculus | |
0.76 | - |
dTTP | pH 8.0, 30°C | Mus musculus | |
0.76 | - |
dUTP | pH 8.0, 30°C | Mus musculus | |
0.87 | - |
5-hydroxy-dCTP | pH 8.0, 30°C | Mus musculus | |
0.94 | - |
5-iodo-dCTP | pH 8.0, 30°C | Mus musculus | |
1.33 | - |
5-Methyl-dCTP | pH 8.0, 30°C | Mus musculus | |
1.37 | - |
dCTP | pH 8.0, 30°C | Mus musculus | |
1.39 | - |
2-hydroxy-dATP | pH 8.0, 30°C | Mus musculus | |
1.49 | - |
5-bromo-dCTP | pH 8.0, 30°C | Mus musculus | |
1.5 | - |
5-chloro-dCTP | pH 8.0, 30°C | Mus musculus | |
1.57 | - |
2-chloro-dATP | pH 8.0, 30°C | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Mus musculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.156 | - |
CTP | pH 8.0, 30°C | Mus musculus | |
1.57 | - |
dUTP | pH 8.0, 30°C | Mus musculus | |
1.87 | - |
dTTP | pH 8.0, 30°C | Mus musculus | |
1.9 | - |
5-fluoro-dUTP | pH 8.0, 30°C | Mus musculus | |
4.49 | - |
dATP | pH 8.0, 30°C | Mus musculus | |
5.3 | - |
5-hydroxy-dCTP | pH 8.0, 30°C | Mus musculus | |
8.22 | - |
2-hydroxy-dATP | pH 8.0, 30°C | Mus musculus | |
14.7 | - |
2-chloro-dATP | pH 8.0, 30°C | Mus musculus | |
27.4 | - |
5-Methyl-dCTP | pH 8.0, 30°C | Mus musculus | |
30 | - |
5-chloro-dCTP | pH 8.0, 30°C | Mus musculus | |
31.1 | - |
dCTP | pH 8.0, 30°C | Mus musculus | |
68.7 | - |
5-bromo-dCTP | pH 8.0, 30°C | Mus musculus | |
241 | - |
5-iodo-dCTP | pH 8.0, 30°C | Mus musculus |