BRENDA - Enzyme Database show
show all sequences of 3.6.1.12

Triptolide directly inhibits dCTP pyrophosphatase

Corson, T.W.; Cavga, H.; Aberle, N.; Crews, C.M.; ChemBioChem 12, 1767-1773 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21 Star (DE3) cells
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
14-alpha-triptolide
-
Homo sapiens
5-hydroxytriptonide
-
Homo sapiens
triptolide
noncompetitive inhibition
Homo sapiens
triptonide
-
Homo sapiens
triptriolide
-
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.239
-
5-iodo-dCTP
in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18000
-
2 * 18000, SDS-PAGE
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
glutathione-Sepharose column chromatography and Mono Q column chromatography
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-iodo-dCTP + H2O
good substrate
719287
Homo sapiens
5-iodo-dCMP + diphosphate
-
-
-
?
additional information
gemcitabine triphosphate is no substrate
719287
Homo sapiens
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homodimer
2 * 18000, SDS-PAGE
Homo sapiens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.000864
-
5-iodo-dCTP
in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
Homo sapiens
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.085
-
triptriolide
in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
Homo sapiens
0.135
-
triptonide
in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
Homo sapiens
0.168
-
triptolide
in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
Homo sapiens
0.356
-
5-hydroxytriptonide
in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21 Star (DE3) cells
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
14-alpha-triptolide
-
Homo sapiens
5-hydroxytriptonide
-
Homo sapiens
triptolide
noncompetitive inhibition
Homo sapiens
triptonide
-
Homo sapiens
triptriolide
-
Homo sapiens
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.085
-
triptriolide
in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
Homo sapiens
0.135
-
triptonide
in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
Homo sapiens
0.168
-
triptolide
in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
Homo sapiens
0.356
-
5-hydroxytriptonide
in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.239
-
5-iodo-dCTP
in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18000
-
2 * 18000, SDS-PAGE
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
glutathione-Sepharose column chromatography and Mono Q column chromatography
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-iodo-dCTP + H2O
good substrate
719287
Homo sapiens
5-iodo-dCMP + diphosphate
-
-
-
?
additional information
gemcitabine triphosphate is no substrate
719287
Homo sapiens
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 18000, SDS-PAGE
Homo sapiens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.000864
-
5-iodo-dCTP
in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
Homo sapiens
Expression
Organism
Commentary
Expression
Homo sapiens
neither knockdown nor over-expression of DCTPP1 significantly modifies the fraction of anti-BrdU reactive cells
additional information
Expression (protein specific)
Organism
Commentary
Expression
Homo sapiens
neither knockdown nor over-expression of DCTPP1 significantly modifies the fraction of anti-BrdU reactive cells
additional information
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
35
-
5-iodo-dCTP
in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
Homo sapiens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
35
-
5-iodo-dCTP
in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
Homo sapiens
Other publictions for EC 3.6.1.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734816
Song
Human dCTP pyrophosphatase 1 p ...
Homo sapiens
Oncogenesis
4
e159
2015
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1
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7
-
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2
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3
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8
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7
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1
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1
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7
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3
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8
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7
-
1
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1
1
-
7
7
733297
Requena
The NTP pyrophosphatase DCTPP1 ...
Homo sapiens
Biochem. J.
459
171-180
2014
-
-
1
-
1
-
-
8
-
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1
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2
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1
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3
-
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9
1
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8
-
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-
-
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1
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1
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8
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1
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1
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3
-
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9
1
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8
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1
1
-
8
8
733777
Zhang
dCTP pyrophosphohydrase exhibi ...
Homo sapiens
Eur. J. Histochem.
57
e29
2013
-
1
-
-
-
-
-
-
1
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2
-
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1
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1
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1
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1
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-
719287
Corson
Triptolide directly inhibits d ...
Homo sapiens
ChemBioChem
12
1767-1773
2011
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1
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5
1
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1
-
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1
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1
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-
-
-
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2
1
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1
-
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4
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1
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5
4
1
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1
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1
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-
-
2
1
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1
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-
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1
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1
1
1
722230
Nonaka
Mouse RS21-C6 is a mammalian 2 ...
Mus musculus
FEBS J.
276
1654-1666
2009
-
-
1
-
-
-
1
13
1
3
2
-
-
2
-
-
-
-
-
3
-
-
14
1
1
-
-
13
1
-
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1
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-
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-
1
-
13
1
3
2
-
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-
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3
-
-
14
1
1
-
-
13
1
-
-
-
-
-
-
-
13
13
209857
Mosig
Several new bacteriophage t4 g ...
Escherichia virus T4
Gene
223
143-155
1998
-
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1
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1
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1
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209858
Gary
A species barrier between bact ...
Enterobacteria phage T2, Escherichia virus T4
Genetics
148
1461-1473
1998
-
-
2
-
-
-
-
-
-
-
-
2
-
4
-
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4
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4
-
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209853
Neale
Enzymes of pyrimidine deoxyrib ...
Mycoplasma mycoides
J. Bacteriol.
156
1001-1005
1983
-
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1
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3
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209854
Allen
T4 phage deoxyribonucleotide-s ...
Escherichia virus T4
J. Biol. Chem.
258
5746-5753
1983
-
-
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1
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1
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1
1
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209855
Chiu
Characteristics of a bacteriop ...
Escherichia virus T4
J. Biol. Chem.
257
15087-15097
1982
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209856
Zimmerman
Deoxycytidine di- and triphosp ...
Enterobacteria phage T2
J. Biol. Chem.
236
1480-1486
1961
-
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