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Literature summary for 3.6.1.12 extracted from

  • Corson, T.W.; Cavga, H.; Aberle, N.; Crews, C.M.
    Triptolide directly inhibits dCTP pyrophosphatase (2011), ChemBioChem, 12, 1767-1773.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
14-alpha-triptolide
-
Homo sapiens
5-hydroxytriptonide
-
Homo sapiens
triptolide noncompetitive inhibition Homo sapiens
triptonide
-
Homo sapiens
triptriolide
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.239
-
5-iodo-dCTP in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
2 * 18000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose column chromatography and Mono Q column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-iodo-dCTP + H2O good substrate Homo sapiens 5-iodo-dCMP + diphosphate
-
?
additional information gemcitabine triphosphate is no substrate Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 18000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
dCTP pyrophosphatase 1
-
Homo sapiens
DCTPP1 formerly known as XTP3TPA Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000864
-
5-iodo-dCTP in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.085
-
triptriolide in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C Homo sapiens
0.135
-
triptonide in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C Homo sapiens
0.168
-
triptolide in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C Homo sapiens
0.356
-
5-hydroxytriptonide in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C Homo sapiens

Expression

Organism Comment Expression
Homo sapiens neither knockdown nor over-expression of DCTPP1 significantly modifies the fraction of anti-BrdU reactive cells additional information

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
35
-
5-iodo-dCTP in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C Homo sapiens