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Literature summary for 3.6.1.11 extracted from

  • Rivero, M.; Torres-Paris, C.; Munoz, R.; Cabrera, R.; Navarro, C.A.; Jerez, C.A.
    Inorganic polyphosphate, exopolyphosphatase, and Pho84-like transporters may be involved in copper resistance in Metallosphaera sedula DSM 5348T (2018), Archaea, 2018, 5251061 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.11

Cloned(Commentary)

Cloned (Comment) Organism
gene ppx or Msed_0891, quantitative RT-PCR expression analysis, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Metallosphaera sedula

Protein Variants

Protein Variants Comment Organism
E111A site-directed mutagenesis, inactive mutant Metallosphaera sedula
E112A site-directed mutagenesis, the mutant shows unaltered PPX activity compared to wild-type Metallosphaera sedula
E113A site-directed mutagenesis, inactive mutant Metallosphaera sedula

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Metallosphaera sedula

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Metallosphaera sedula

Organism

Organism UniProt Comment Textmining
Metallosphaera sedula A4YFE8
-
-
Metallosphaera sedula ATCC 51363 A4YFE8
-
-
Metallosphaera sedula DSM 5348 A4YFE8
-
-
Metallosphaera sedula JCM 9185 A4YFE8
-
-
Metallosphaera sedula NBRC 15509 A4YFE8
-
-
Metallosphaera sedula TH2 A4YFE8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Metallosphaera sedula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the purified recombinant enzyme hydrolyzes polyphosphate, E111 and E113 are essential residues for catalysis of PPXMsed Metallosphaera sedula ?
-
-
additional information the purified recombinant enzyme hydrolyzes polyphosphate, E111 and E113 are essential residues for catalysis of PPXMsed Metallosphaera sedula NBRC 15509 ?
-
-
additional information the purified recombinant enzyme hydrolyzes polyphosphate, E111 and E113 are essential residues for catalysis of PPXMsed Metallosphaera sedula TH2 ?
-
-
additional information the purified recombinant enzyme hydrolyzes polyphosphate, E111 and E113 are essential residues for catalysis of PPXMsed Metallosphaera sedula JCM 9185 ?
-
-
additional information the purified recombinant enzyme hydrolyzes polyphosphate, E111 and E113 are essential residues for catalysis of PPXMsed Metallosphaera sedula DSM 5348 ?
-
-
additional information the purified recombinant enzyme hydrolyzes polyphosphate, E111 and E113 are essential residues for catalysis of PPXMsed Metallosphaera sedula ATCC 51363 ?
-
-

Synonyms

Synonyms Comment Organism
Msed_0981 locus name Metallosphaera sedula
PPX
-
 Metallosphaera sedula
Ppx/GppA phosphatase UniProt Metallosphaera sedula
PPXMsed
-
 Metallosphaera sedula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Metallosphaera sedula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Metallosphaera sedula

Expression

Organism Comment Expression
Metallosphaera sedula gene ppx is upregulated upon copper exposure up

General Information

General Information Comment Organism
metabolism inorganic polyphosphate, exopolyphosphatase, and Pho84-like transporters may be involved in copper resistance in Metallosphaera sedula DSM 5348. Existence of a polyphosphate-dependent copper-resistance system that may be of great importance in the adaptation of this thermoacidophilic archaeon to its harsh environment Metallosphaera sedula
additional information homology modeling structure modelling, Aquifex aeolicus PPX structure (PDB ID 1T6C) is used as the template, overview Metallosphaera sedula