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Literature summary for 3.6.1.11 extracted from

  • Andreeva, N.; Trilisenko, L.; Eldarov, M.; Kulakovskaya, T.
    Polyphosphatase PPN1 of Saccharomyces cerevisiae: switching of exopolyphosphatase and endopolyphosphatase activities (2015), PLoS ONE, 10, e0119594.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
additional information in the presence of Mg2+, the enzyme shows a polyphosphate chain fragmentation activity. ATP inhibits while ADP activates endopolyphosphatase activity in the presence of Mg2+ Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ Co2+ stimulates phosphate release from the polyphosphate chain end Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q04119 bifunctional exo- and endopolyphosphatase, EC 3.6.1.11 and EC 3.6.1.10
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification PPN1 gene encodes a polypeptide with the predicted molecular mass of 78 kDa, the monomer molecular mass of the mature PPN1 is about 33-35 kDa Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3 8 substrate (phosphate)3, presence of 0.1 mM Co2+, pH 7.2, 30°C Saccharomyces cerevisiae
240
-
 substrate (phosphate)15, presence of 0.1 mM Co2+, pH 7.2, 30°C Saccharomyces cerevisiae
290
-
 substrate (phosphate)208, presence of 0.1 mM Co2+, pH 7.2, 30°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(phosphate)15 + H2O
-
 Saccharomyces cerevisiae (phosphate)14 + phosphate
-
 ?
(phosphate)208 + H2O
-
 Saccharomyces cerevisiae (phosphate)207 + phosphate reaction continues to a chain length of about 15 residues ?
(phosphate)3 + H2O
-
 Saccharomyces cerevisiae (phosphate)2 + phosphate
-
 ?
additional information enzyme is a bifunctional exo- and endopolyphosphatase, activities of EC 3.6.1.11 and EC 3.6.1.10, respectively. No activity with diphosphate, ATP and 4-nitrophenylphosphate Saccharomyces cerevisiae ?
-
 ?