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Literature summary for 3.6.1.11 extracted from

  • Andreeva, N.; Trilisenko, L.; Kulakovskaya, T.; Dumina, M.; Eldarov, M.
    Purification and properties of recombinant exopolyphosphatase PPN1 and effects of its overexpression on polyphosphate in Saccharomyces cerevisiae (2015), J. Biosci. Bioeng., 119, 52-56.
    View publication on PubMed
Search for more literature for 3.6.1.11

Activating Compound

Activating Compound Comment Organism Structure
ammonium chloride 200 mM, stimulates almost twofold in the presence and absence of 0.1 mM Co2+ Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
heparin
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol 42% of the activity in the lysate Saccharomyces cerevisiae 5829
-
vacuole 20% of the activity in the lysate Saccharomyces cerevisiae 5773
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ best activator, maximum activation at 0.1 mM Saccharomyces cerevisiae
Mg2+ poor activation Saccharomyces cerevisiae
Mn2+ poor activition Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 x * 33000, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q04119 bifunctional exo- and endopolyphosphatase, ECs 3.6.1.10 and 3.6.1.11
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(phosphate)15 + H2O 88% of the activity with (phosphate)25 Saccharomyces cerevisiae (phosphate)14 + phosphate
-
 ?
(phosphate)208 + H2O 100% activity Saccharomyces cerevisiae (phosphate)207 + phosphate
-
 ?
(phosphate)25 + H2O 100% activity Saccharomyces cerevisiae (phosphate)24 + phosphate
-
 ?
(phosphate)3 + H2O 14% of the activity with (phosphate)25 Saccharomyces cerevisiae (phosphate)2 + phosphate
-
 ?
(phosphate)45 + H2O 100% activity Saccharomyces cerevisiae (phosphate)44 + phosphate
-
 ?
(phosphate)9 + H2O 45% of the activity with (phosphate)25 Saccharomyces cerevisiae (phosphate)8 + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 33000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Ppn1
-
 Saccharomyces cerevisiae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
 50% inhibition around 1 mg/L Saccharomyces cerevisiae heparin

General Information

General Information Comment Organism
physiological function strong constitutive overexpression of exopolyphosphatase PPN1 results in 28- and 11fold increase in activity compared to the PPN1 mutant and wild-type strains, respectively. The content of acid-soluble polyphosphate decreases about 6fold and the content of acid-insoluble polyphosphate decreases about 2.5fold in the cells of the transformant compared to the mutant strain Saccharomyces cerevisiae