Literature summary for 3.6.1.11 extracted from

Lichko, L.P.; Kulakovskaya, T.V.; Kulaev, I.S.
Two exopolyphosphatases of Microlunatus phosphovorus, a polyphosphate-accumulating eubacterium from activated sludge (2002), Process Biochem., 37, 799-803.

No PubMed abstract available

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Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	45% inhibition of polyphosphatase I and 55% inhibition of polyphosphatase II, both at 0.3 mM	Microlunatus phosphovorus
heparin	complete inhibition of polyphosphatase I at 0.05 mg/ml, 35% inhibition of polyphosphatase II at 0.1 mg/ml	Microlunatus phosphovorus
additional information	NaF and vanadate are not inhibitors	Microlunatus phosphovorus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	activates 4.4fold polyphosphatase II and activates 1.5fold polyphosphatase I both at 0.15 mM	Microlunatus phosphovorus
K+	activates 2fold polyphosphatase II, but not activates polyphosphatase I	Microlunatus phosphovorus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	polyphosphatase II, gel filtration	Microlunatus phosphovorus
93000	-	polyphosphatase I, gel filtration	Microlunatus phosphovorus

Organism

Organism	UniProt	Comment	Textmining
Microlunatus phosphovorus	-	NM-1 strain, two isoenzymes polyphosphatases I and II	-

Purification (Commentary)

Purification (Comment)	Organism
isolation of two isoenzymes by cellular fractionation and gel filtration	Microlunatus phosphovorus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(polyphosphate)n + H2O	polyphosphatase I shows optimal activity when n is 25 phosphate residues, polyphosphatases II prefers substrates with 9 residues	Microlunatus phosphovorus	(polyphosphate)n-1 + phosphate	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	-	polyphosphatase I	Microlunatus phosphovorus
7.5	-	polyphosphatase II	Microlunatus phosphovorus

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Release 2023.1 (January 2023)