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Literature summary for 3.6.1.11 extracted from
- Partial purification and characterization of nuclear exopolyphosphatase from Saccharomyces cerevisiae strain with inactivated PPX1 gene encoding a major yeast exopolyphosphatase (2004), Biochemistry, 69, 270-274.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | RNA does not alter the nuclear exopolyphosphatase activity | Saccharomyces cerevisiae | |
| polylysine | 100 mg/l, 2fold activity enhancement | Saccharomyces cerevisiae |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| heparin | 20 mg/l, 85% activity loss; 85% inhibition at 0.02 mg /ml | Saccharomyces cerevisiae | |
| additional information | not inhibited by 1 mM sodium fluoride | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
(Polyphosphate)n | n = 208 | Saccharomyces cerevisiae | |
| 0.025 | - |
(25R)-3beta-hydroxycholest-5-en-27-oate | polyphosphate 208, in the presence of 2.5 mM Mg2+ | Saccharomyces cerevisiae | |
| 0.133 | - |
(Polyphosphate)n | n = 15 | Saccharomyces cerevisiae | |
| 0.133 | - |
(25R)-3beta-hydroxycholest-5-en-27-oate | polyphosphate 15, in the presence of 2.5 mM Mg2+ | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | best activator | Saccharomyces cerevisiae | |
| Co2+ | 0.1 mM, 31fold activity enhancement | Saccharomyces cerevisiae | |
| K+ | slight activation | Saccharomyces cerevisiae | |
| K+ | 100 mM, 30% activity enhancement | Saccharomyces cerevisiae | |
| Mg2+ | 2.5 mM, 15fold activity enhancement | Saccharomyces cerevisiae | |
| NH4+ | slight activation | Saccharomyces cerevisiae | |
| NH4+ | 100 mM, 35% activity enhancement | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 200000 | - |
gel filtration | Saccharomyces cerevisiae |
| 200000 | - |
gel filtration on Superose 6 | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae | P38698 | PPX1 mutant | - |
| Saccharomyces cerevisiae CRX | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion-exchange chromatography | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.04 | - |
1.25 mM diphosphate as substrate, in the presence of 2.5 mM Mg2+ | Saccharomyces cerevisiae |
| 0.05 | - |
1.25 mM polyphosphate 3 as substrate, in the presence of 2.5 mM Mg2+ | Saccharomyces cerevisiae |
| 0.095 | - |
0.01 mM polyphosphate 208 as substrate, in the presence of 2.5 mM Mg2+ | Saccharomyces cerevisiae |
| 0.1 | - |
0.13 mM polyphosphate 15 as substrate, in the presence of 2.5 mM Mg2+ | Saccharomyces cerevisiae |
| 0.21 | - |
0.13 mM polyphosphate 15 as substrate, in the presence of 1 mM Co2+ | Saccharomyces cerevisiae |
| 0.23 | - |
0.13 mM polyphosphate 15 as substrate, in the presence of 0.1 mM Co2+ | Saccharomyces cerevisiae |
| 0.28 | - |
0.01 mM polyphosphate 208 as substrate, in the presence of 1 mM Co2+ | Saccharomyces cerevisiae |
| 0.3 | - |
2.0 mM polyphosphate 208 as substrate, in the presence of 0.1 mM Co2+ | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (25R)-3beta-hydroxycholest-5-en-27-oate + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| (25R)-3beta-hydroxycholest-5-en-27-oate + H2O | - |
Saccharomyces cerevisiae CRX | ? | - |
? | |
| (polyphosphate)n + H2O | - |
Saccharomyces cerevisiae | (polyphosphate)n-1 + phosphate | - |
? | |
| (polyphosphate)n + H2O | - |
Saccharomyces cerevisiae CRX | (polyphosphate)n-1 + phosphate | - |
? | |
| polyphosphate 15 + H2O | - |
Saccharomyces cerevisiae | polyphosphate 14 + phosphate | - |
? | |
| polyphosphate 15 + H2O | - |
Saccharomyces cerevisiae CRX | polyphosphate 14 + phosphate | - |
? | |
| polyphosphate 208 + H2O | - |
Saccharomyces cerevisiae | polyphosphate 207 + phosphate | - |
? | |
| polyphosphate 208 + H2O | - |
Saccharomyces cerevisiae CRX | polyphosphate 207 + phosphate | - |
? | |
| polyphosphate 3 + H2O | - |
Saccharomyces cerevisiae | polyphosphate 2 + phosphate | - |
? | |
| polyphosphate 3 + H2O | - |
Saccharomyces cerevisiae CRX | polyphosphate 2 + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| major cytosolic exopolyphosphatase PPX1 | - |
Saccharomyces cerevisiae |
| nuclear exopolyphosphatase | - |
Saccharomyces cerevisiae |
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