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Literature summary for 3.6.1.11 extracted from

  • Lorenz, B.; Batel, R.; Bachinski, N.; Mueller, W.E.G.; Schroeder, H.C.
    Purification and characterization of two exopolyphosphatases from the marine sponge Tethya lyncurium (1995), Biochim. Biophys. Acta, 1245, 17-28.
    View publication on PubMed
Search for more literature for 3.6.1.11

General Stability

General Stability Organism
repeated freezing and thawing destroys activity Tethya aurantium

Inhibitors

Inhibitors Comment Organism Structure
ADP 0.5 mM, 30% loss of activity, exopolyphosphatase II Tethya aurantium
ATP 0.5 mM, 47% loss of activity, exopolyphosphatase II Tethya aurantium
EDTA exopolyphosphatase II Tethya aurantium
Mg2+ exopolyphosphatase I Tethya aurantium
NaCl 600 mM NaCl, 73% inhibition of exopolyphosphatase I and 84% inhibition of exopolyphosphatase II Tethya aurantium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0092
-
 polyP10 exopolyphosphatase II Tethya aurantium
0.0238
-
 polyP10 exopolyphosphatase I Tethya aurantium

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal activity of exopolyphosphatase II in presence of 3 mM Tethya aurantium
additional information exopolyphosphatase I does not require divalent cations Tethya aurantium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 exopolyphosphatase I, gel filtration Tethya aurantium
70000
-
 exopolyphosphatase II, gel filtration Tethya aurantium
73000
-
 1 * 73000, exopolyphosphatase I, SDS-PAGE Tethya aurantium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(polyphosphate)n + H2O Tethya aurantium the inhibitory effect of long-chain polyphosphates on adenylate kinase is higher than that of short-chain polyphosphates, suggesting a potential role of polyphosphate metabolism in regulating intracellular concentration of adenylate nucleotides (polyphosphate)n-1 + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Tethya aurantium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
exopolyphosphatase I and II Tethya aurantium

Storage Stability

Storage Stability Organism
-20°C, in 50 mM Tris-HCl buffer, pH 7.5, 5 mM MgCl2, 0.5 mM EDTA, 50 mM NaCl, 0.1% w/v bovine serum albumin, 40% v/v glycerol, exopolyphosphatase I and II, less than 30% loss of activity after 2 months Tethya aurantium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(polyphosphate)n + H2O
-
 Tethya aurantium (polyphosphate)n-1 + phosphate
-
 ?
(polyphosphate)n + H2O the inhibitory effect of long-chain polyphosphates on adenylate kinase is higher than that of short-chain polyphosphates, suggesting a potential role of polyphosphate metabolism in regulating intracellular concentration of adenylate nucleotides Tethya aurantium (polyphosphate)n-1 + phosphate
-
 ?
polyP10 + H2O
-
 Tethya aurantium polyP9 + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, exopolyphosphatase I, SDS-PAGE Tethya aurantium
monomer 1 * 73000, exopolyphosphatase I, SDS-PAGE Tethya aurantium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 exopolyphosphatase I Tethya aurantium
7.5
-
 exopolyphosphatase II Tethya aurantium

pH Range

pH Minimum pH Maximum Comment Organism
4.6 5.4 pH 4.6: 81% of maximal activity, pH 5.4: 59% of maximal activity, exopolyphosphatase I Tethya aurantium
7 9 pH 7.0: 5% of maximal activity, pH 9.0: 57% of maximal activity, exopolyphosphatase I Tethya aurantium