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Literature summary for 3.6.1.11 extracted from

  • Wurst, H.; Kornberg, A.
    A soluble exopolyphosphatase of Saccharomyces cerevisiae. Purification and characterization (1994), J. Biol. Chem., 269, 10996-11001.
    View publication on PubMed
Search for more literature for 3.6.1.11

General Stability

General Stability Organism
more than 90% loss of activity after freezing in liquid nitrogen and subsequent thawing Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
CHES buffer 20 mM Saccharomyces cerevisiae
MES buffer 20 mM Saccharomyces cerevisiae
spermidine 0.17 mM, 50% loss of activity Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000004
-
 polyP250
-
 Saccharomyces cerevisiae
0.000024
-
 polyP100
-
 Saccharomyces cerevisiae
0.00005
-
 polyP500
-
 Saccharomyces cerevisiae
0.00006
-
 polyP50
-
 Saccharomyces cerevisiae
0.00016
-
 polyP25
-
 Saccharomyces cerevisiae
0.0039
-
 polyP10
-
 Saccharomyces cerevisiae
0.14
-
 tripolyphosphate
-
 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 2 mM CoCl2, 2.5fold stimulation Saccharomyces cerevisiae
Mg2+ 1-10 mM, about 7fold activation Saccharomyces cerevisiae
Mn2+ 1-10 mM, 3-5fold stimulation Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 gel filtration Saccharomyces cerevisiae
420000
-
 1 * 420000, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(polyphosphate)n + H2O n = 10, 25, 50, 100, 250 or 500. n = 250 is the preferred substrate Saccharomyces cerevisiae (polyphosphate)n-1 + phosphate
-
 ?
polyP10 + H2O
-
 Saccharomyces cerevisiae polyP9 + phosphate
-
 ?
polyP100 + H2O
-
 Saccharomyces cerevisiae polyP99 + phosphate
-
 ?
polyP25 + H2O
-
 Saccharomyces cerevisiae polyP24 + phosphate
-
 ?
polyP250 + H2O
-
 Saccharomyces cerevisiae polyP249 + phosphate
-
 ?
polyP50 + H2O
-
 Saccharomyces cerevisiae polyP49 + phosphate
-
 ?
polyP500
-
 Saccharomyces cerevisiae polyP499 + phosphate
-
 ?
polyP500 + H2O
-
 Saccharomyces cerevisiae polyP499 + phosphate
-
 ?
tripolyphosphate + H2O
-
 Saccharomyces cerevisiae phosphate + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 420000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
47
-
-
 Saccharomyces cerevisiae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 53 30°C: about 30% of maximal activity, 53°C: about 40% of maximal activity Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 polyP500
-
 Saccharomyces cerevisiae
1.2
-
 polyP250
-
 Saccharomyces cerevisiae
3.3
-
 polyP100
-
 Saccharomyces cerevisiae
8.4
-
 polyP50
-
 Saccharomyces cerevisiae
15
-
 polyP25
-
 Saccharomyces cerevisiae
63
-
 polyP10
-
 Saccharomyces cerevisiae
180
-
 tripolyphosphate
-
 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 8.8
-
 Saccharomyces cerevisiae