Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3) | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0822 | - |
polyphosphate60 | pH 7.4, 37°C, recombinant enzyme | Trypanosoma brucei | |
0.1494 | - |
polyphosphate700 | pH 7.4, 37°C, recombinant enzyme | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Trypanosoma brucei | 5829 | - |
nucleus | - |
Trypanosoma brucei | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Trypanosoma brucei | |
Mn2+ | required | Trypanosoma brucei | |
additional information | Mg2+ or Mn2+ are the preferred cofactors while no activity is detected with Co2+ | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
polyphosphate60 + H2O | Trypanosoma brucei | - |
? | - |
? | |
polyphosphate60 + H2O | Trypanosoma brucei 927/4 GUTat10.1 | - |
? | - |
? | |
polyphosphate700 + H2O | Trypanosoma brucei | - |
? | - |
? | |
polyphosphate700 + H2O | Trypanosoma brucei 927/4 GUTat10.1 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | Q583R7 | - |
- |
Trypanosoma brucei 927/4 GUTat10.1 | Q583R7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus (DE3) by nickel affinity chromatography and dialysis | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | TbNH4 is an endo- and exopolyphosphatase that has similar activity on polyP of various chain sizes. The enzyme has a higher affinity for polyP60 than for polyphosphate700. No activity with 5-diphosphoinositol pentakisphosphate (5-IP7). Malachite green assay | Trypanosoma brucei | ? | - |
- |
|
additional information | TbNH4 is an endo- and exopolyphosphatase that has similar activity on polyP of various chain sizes. The enzyme has a higher affinity for polyP60 than for polyphosphate700. No activity with 5-diphosphoinositol pentakisphosphate (5-IP7). Malachite green assay | Trypanosoma brucei 927/4 GUTat10.1 | ? | - |
- |
|
polyphosphate60 + H2O | - |
Trypanosoma brucei | ? | - |
? | |
polyphosphate60 + H2O | - |
Trypanosoma brucei 927/4 GUTat10.1 | ? | - |
? | |
polyphosphate700 + H2O | - |
Trypanosoma brucei | ? | - |
? | |
polyphosphate700 + H2O | - |
Trypanosoma brucei 927/4 GUTat10.1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endopolyphosphatase | - |
Trypanosoma brucei |
More | see also EC 3.6.1.11 | Trypanosoma brucei |
Nudix hydrolase | - |
Trypanosoma brucei |
Tb927.6.2670 | - |
Trypanosoma brucei |
TbDcp2 | - |
Trypanosoma brucei |
TbNH4 | - |
Trypanosoma brucei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trypanosoma brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
73628 | - |
polyphosphate700 | pH 7.4, 37°C, recombinant enzyme | Trypanosoma brucei | |
413625 | - |
polyphosphate60 | pH 7.4, 37°C, recombinant enzyme | Trypanosoma brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Trypanosoma brucei |
General Information | Comment | Organism |
---|---|---|
evolution | the Nudix superfamily (Pfam PF00293) is found in archaea, bacteria, eukaryotes and viruses and includes pyrophosphohydrolases of nucleotide sugars and alcohols, nucleoside and deoxynucleoside triphosphates ([d]NTPs), dinucleoside polyphosphates, dinucleotide coenzymes and capped RNAs. Trypanosoma brucei has five Nudix hydrolases | Trypanosoma brucei |
physiological function | NHs can participate in polyphosphate homeostasis and therefore may help control polyphosphate levels in glycosomes, cytosol and nuclei of Trypanosoma brucei. Endopolyphosphatase (PPN) activity cleaves internal phosphoanhydride bonds generating shorter polyphosphate molecules. Nudix hydrolase 4 (TbNH4 or TbDcp2) is a mRNA de-capping enzyme that removes the 5' cap from processed mRNAs | Trypanosoma brucei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11000 | - |
polyphosphate700 | pH 7.4, 37°C, recombinant enzyme | Trypanosoma brucei | |
34000 | - |
polyphosphate60 | pH 7.4, 37°C, recombinant enzyme | Trypanosoma brucei |