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Literature summary for 3.6.1.10 extracted from

  • Cordeiro, C.D.; Ahmed, M.A.; Windle, B.; Docampo, R.
    NUDIX hydrolases with inorganic polyphosphate exo- and endopolyphosphatase activities in the glycosome, cytosol and nucleus of Trypanosoma brucei (2019), Biosci. Rep., 39, BSR20190894 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3) Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0822
-
polyphosphate60 pH 7.4, 37°C, recombinant enzyme Trypanosoma brucei
0.1494
-
polyphosphate700 pH 7.4, 37°C, recombinant enzyme Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Trypanosoma brucei 5829
-
nucleus
-
Trypanosoma brucei 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Trypanosoma brucei
Mn2+ required Trypanosoma brucei
additional information Mg2+ or Mn2+ are the preferred cofactors while no activity is detected with Co2+ Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polyphosphate60 + H2O Trypanosoma brucei
-
?
-
?
polyphosphate60 + H2O Trypanosoma brucei 927/4 GUTat10.1
-
?
-
?
polyphosphate700 + H2O Trypanosoma brucei
-
?
-
?
polyphosphate700 + H2O Trypanosoma brucei 927/4 GUTat10.1
-
?
-
?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei Q583R7
-
-
Trypanosoma brucei 927/4 GUTat10.1 Q583R7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus (DE3) by nickel affinity chromatography and dialysis Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information TbNH4 is an endo- and exopolyphosphatase that has similar activity on polyP of various chain sizes. The enzyme has a higher affinity for polyP60 than for polyphosphate700. No activity with 5-diphosphoinositol pentakisphosphate (5-IP7). Malachite green assay Trypanosoma brucei ?
-
-
additional information TbNH4 is an endo- and exopolyphosphatase that has similar activity on polyP of various chain sizes. The enzyme has a higher affinity for polyP60 than for polyphosphate700. No activity with 5-diphosphoinositol pentakisphosphate (5-IP7). Malachite green assay Trypanosoma brucei 927/4 GUTat10.1 ?
-
-
polyphosphate60 + H2O
-
Trypanosoma brucei ?
-
?
polyphosphate60 + H2O
-
Trypanosoma brucei 927/4 GUTat10.1 ?
-
?
polyphosphate700 + H2O
-
Trypanosoma brucei ?
-
?
polyphosphate700 + H2O
-
Trypanosoma brucei 927/4 GUTat10.1 ?
-
?

Synonyms

Synonyms Comment Organism
endopolyphosphatase
-
Trypanosoma brucei
More see also EC 3.6.1.11 Trypanosoma brucei
Nudix hydrolase
-
Trypanosoma brucei
Tb927.6.2670
-
Trypanosoma brucei
TbDcp2
-
Trypanosoma brucei
TbNH4
-
Trypanosoma brucei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
73628
-
polyphosphate700 pH 7.4, 37°C, recombinant enzyme Trypanosoma brucei
413625
-
polyphosphate60 pH 7.4, 37°C, recombinant enzyme Trypanosoma brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Trypanosoma brucei

General Information

General Information Comment Organism
evolution the Nudix superfamily (Pfam PF00293) is found in archaea, bacteria, eukaryotes and viruses and includes pyrophosphohydrolases of nucleotide sugars and alcohols, nucleoside and deoxynucleoside triphosphates ([d]NTPs), dinucleoside polyphosphates, dinucleotide coenzymes and capped RNAs. Trypanosoma brucei has five Nudix hydrolases Trypanosoma brucei
physiological function NHs can participate in polyphosphate homeostasis and therefore may help control polyphosphate levels in glycosomes, cytosol and nuclei of Trypanosoma brucei. Endopolyphosphatase (PPN) activity cleaves internal phosphoanhydride bonds generating shorter polyphosphate molecules. Nudix hydrolase 4 (TbNH4 or TbDcp2) is a mRNA de-capping enzyme that removes the 5' cap from processed mRNAs Trypanosoma brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11000
-
polyphosphate700 pH 7.4, 37°C, recombinant enzyme Trypanosoma brucei
34000
-
polyphosphate60 pH 7.4, 37°C, recombinant enzyme Trypanosoma brucei