Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 89% activation at 0.9 mM, complete inhibition at 5 mM | Hypophthalmichthys nobilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 89% activation at 0.9 mM, complete inhibition at 5 mM | Hypophthalmichthys nobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.98 | - |
diphosphate | pH 7.0, 50°C, in presence of 5 mM Mg2+ | Hypophthalmichthys nobilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 60.1% of the activity with Mg2+ | Hypophthalmichthys nobilis | |
Mg2+ | most effetive divalent cation, can partially be replaced by other divalent cations | Hypophthalmichthys nobilis | |
Mn2+ | 32.8% of the activity with Mg2+ | Hypophthalmichthys nobilis | |
additional information | no effect by Ca2+, Cu2+, and Fe2+ | Hypophthalmichthys nobilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
2 * 25000, SDS-PAGE | Hypophthalmichthys nobilis |
50000 | - |
gel filtration | Hypophthalmichthys nobilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Hypophthalmichthys nobilis | - |
2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hypophthalmichthys nobilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from muscles, 114.5fold by ammonium sulfate fractionation, dialysis, ion exchange chromatography, and gel filtration to homogeneity | Hypophthalmichthys nobilis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Hypophthalmichthys nobilis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.52 | - |
purified native enzyme | Hypophthalmichthys nobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Hypophthalmichthys nobilis | 2 phosphate | - |
? | |
additional information | no activity with sodium triphosphate, ADP, ATP, and glucose-6-phosphate | Hypophthalmichthys nobilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 25000, SDS-PAGE | Hypophthalmichthys nobilis |
Synonyms | Comment | Organism |
---|---|---|
PPase | - |
Hypophthalmichthys nobilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Hypophthalmichthys nobilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | - |
Hypophthalmichthys nobilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
purified enzyme, quite stable | Hypophthalmichthys nobilis |
30 | - |
60 min, purified enzyme, 54% remaining activity | Hypophthalmichthys nobilis |
40 | - |
30 min, purified enzyme, 61% remaining activity | Hypophthalmichthys nobilis |
50 | - |
inactivation above | Hypophthalmichthys nobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Hypophthalmichthys nobilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 12 | - |
Hypophthalmichthys nobilis |