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Literature summary for 3.6.1.1 extracted from

  • Gao, R.; Xue, C.; Yuan, L.; Zhang, J.; Li, Z.; Xue, Y.; Feng, H.
    Purification and characterization of pyrophosphatase from bighead carp (Aristichthys nobilis) (2008), Lebensm. Wiss. Technol., 41, 254-261.
    View publication on PubMed
Search for more literature for 3.6.1.1

Activating Compound

Activating Compound Comment Organism Structure
EDTA 89% activation at 0.9 mM, complete inhibition at 5 mM Hypophthalmichthys nobilis

Inhibitors

Inhibitors Comment Organism Structure
EDTA 89% activation at 0.9 mM, complete inhibition at 5 mM Hypophthalmichthys nobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.98
-
 diphosphate pH 7.0, 50°C, in presence of 5 mM Mg2+ Hypophthalmichthys nobilis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 60.1% of the activity with Mg2+ Hypophthalmichthys nobilis
Mg2+ most effetive divalent cation, can partially be replaced by other divalent cations Hypophthalmichthys nobilis
Mn2+ 32.8% of the activity with Mg2+ Hypophthalmichthys nobilis
additional information no effect by Ca2+, Cu2+, and Fe2+ Hypophthalmichthys nobilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 2 * 25000, SDS-PAGE Hypophthalmichthys nobilis
50000
-
 gel filtration Hypophthalmichthys nobilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Hypophthalmichthys nobilis
-
 2 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Hypophthalmichthys nobilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from muscles, 114.5fold by ammonium sulfate fractionation, dialysis, ion exchange chromatography, and gel filtration to homogeneity Hypophthalmichthys nobilis

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Hypophthalmichthys nobilis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.52
-
 purified native enzyme Hypophthalmichthys nobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
 Hypophthalmichthys nobilis 2 phosphate
-
 ?
additional information no activity with sodium triphosphate, ADP, ATP, and glucose-6-phosphate Hypophthalmichthys nobilis ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 25000, SDS-PAGE Hypophthalmichthys nobilis

Synonyms

Synonyms Comment Organism
PPase
-
 Hypophthalmichthys nobilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Hypophthalmichthys nobilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60
-
 Hypophthalmichthys nobilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 purified enzyme, quite stable Hypophthalmichthys nobilis
30
-
 60 min, purified enzyme, 54% remaining activity Hypophthalmichthys nobilis
40
-
 30 min, purified enzyme, 61% remaining activity Hypophthalmichthys nobilis
50
-
 inactivation above Hypophthalmichthys nobilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Hypophthalmichthys nobilis

pH Range

pH Minimum pH Maximum Comment Organism
4 12
-
 Hypophthalmichthys nobilis