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Literature summary for 3.6.1.1 extracted from
- Metal-free PPi activates hydrolysis of MgPPi by an Escherichia coli inorganic pyrophosphatase (2005), Biochemistry (Moscow), 70, 69-78.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| methylene diphosphate | noncompetitive activation of magnesium diphosphate hydrolysis | Escherichia coli |  |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D26A | site-directed mutagenesis, the mutant does not obey Michaelis-Menten kinetics | Escherichia coli |
| E145Q | site-directed mutagenesis, about 80% reduced activity and altered kinetic properties compared to the wild-type enzyme | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methylene diphosphate-Mg complex | competitive inhibition of MgPPi hydrolysis, binds at the active site | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | detailed kinetics of hexameric, trimeric enzyme, and enzyme mutants, overview, the active trimeric enzyme, formed under acidic conditions, does not obey Michaelis-Menten kinetics, overview | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Escherichia coli | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, substrate is magnesium diphoshate | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | Escherichia coli | - |
2 phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
| 15 | - |
hexameric mutant E145Q | Escherichia coli |
| 280 | - |
wild-type enzyme | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | - |
Escherichia coli | 2 phosphate | - |
? | |
| diphosphate + H2O | MgPPi | Escherichia coli | 2 phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | dimer of active trimers | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| inorganic pyrophosphatase | - |
Escherichia coli |
| PPase | - |
Escherichia coli |
| soluble inorganic pyrophosphatase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 83 | - |
diphosphate | pH 7.5, 25°C, hexameric mutant E145Q, 5 mM Mg2+ | Escherichia coli | |
| 390 | - |
diphosphate | pH 7.5, 25°C, wild-type enzyme, 5 mM Mg2+ | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH-dependence of wild-type and mutant activity, overview | Escherichia coli |
| 7.5 | - |
assay at | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.7 | - |
Escherichia coli |
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