General Stability | Organism |
---|---|
extremely stable. No dissociation into subunit or unfolding of polypeptide chain occurrs in the presence of 8 M | Sulfolobus acidocaldarius |
urea. Unfolding using guanidine-HC1 suggests that the transition between a native tetrameric state and an unfolded state is completely reversible, and essentially independent of any additional factors such as divalent metal cation or dithiothreitol | Sulfolobus acidocaldarius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 50% at 0.012 mM: Mg2+ 0.4 mM | Sulfolobus acidocaldarius | |
diphosphate | free | Sulfolobus acidocaldarius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Sulfolobus acidocaldarius | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Sulfolobus acidocaldarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
4 * 21000, SDS-PAGE | Sulfolobus acidocaldarius |
80000 | - |
gel filtration | Sulfolobus acidocaldarius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Sulfolobus acidocaldarius | actual substrate is magnesium diphosphate or dimagnesium diphosphate | 2 phosphate | - |
? | |
diphosphate + H2O | Sulfolobus acidocaldarius 7 | actual substrate is magnesium diphosphate or dimagnesium diphosphate | 2 phosphate | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | 20% v/v, 82% residual activity | Sulfolobus acidocaldarius |
isopropanol | 20% v/v, 82% residual activity | Sulfolobus acidocaldarius |
Methanol | 20% v/v, 82% residual activity | Sulfolobus acidocaldarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | - |
- |
- |
Sulfolobus acidocaldarius 7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus acidocaldarius |
Renatured (Comment) | Organism |
---|---|
unfolding using guanidine-HCl suggests that the transition between a native tetrameric state and an unfolded state is completely reversible, and essentially independent of any additional factors such as divalent metal cation or dithiothreitol | Sulfolobus acidocaldarius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
617 | - |
56°C | Sulfolobus acidocaldarius |
617 | - |
pH 6.5, 56°C | Sulfolobus acidocaldarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Sulfolobus acidocaldarius | 2 phosphate | - |
? | |
diphosphate + H2O | actual substrate is magnesium diphosphate or dimagnesium diphosphate | Sulfolobus acidocaldarius | 2 phosphate | - |
? | |
diphosphate + H2O | high specificity | Sulfolobus acidocaldarius | 2 phosphate | - |
? | |
diphosphate + H2O | - |
Sulfolobus acidocaldarius 7 | 2 phosphate | - |
? | |
diphosphate + H2O | actual substrate is magnesium diphosphate or dimagnesium diphosphate | Sulfolobus acidocaldarius 7 | 2 phosphate | - |
? | |
diphosphate + H2O | high specificity | Sulfolobus acidocaldarius 7 | 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | analysis of amino acid composition and comparison with Escherichia coli, saccharomyces cerevisiae and Bacillus sp. enzymes | Sulfolobus acidocaldarius |
tetramer | 4 * 21000, SDS-PAGE | Sulfolobus acidocaldarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Sulfolobus acidocaldarius |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 100 | - |
Sulfolobus acidocaldarius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
10 min, no loss of activity | Sulfolobus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Sulfolobus acidocaldarius |
6.5 | - |
56°C | Sulfolobus acidocaldarius |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sulfolobus acidocaldarius | isoelectric focusing | - |
5.1 |