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Literature summary for 3.6.1.1 extracted from

  • Wakagi, T.; Lee, C.H.; Oshima, T.
    An extemely stable inorganic pyrophosphatase purified from the cytosol of a thermoacidophilic archaebacterium, Sulfolobus acidcaldarius strain 7 (1992), Biochim. Biophys. Acta, 1120, 289-296.
    View publication on PubMed

General Stability

General Stability Organism
extremely stable. No dissociation into subunit or unfolding of polypeptide chain occurrs in the presence of 8 M Sulfolobus acidocaldarius
urea. Unfolding using guanidine-HC1 suggests that the transition between a native tetrameric state and an unfolded state is completely reversible, and essentially independent of any additional factors such as divalent metal cation or dithiothreitol Sulfolobus acidocaldarius

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 50% at 0.012 mM: Mg2+ 0.4 mM Sulfolobus acidocaldarius
diphosphate free Sulfolobus acidocaldarius

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Sulfolobus acidocaldarius 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Sulfolobus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
4 * 21000, SDS-PAGE Sulfolobus acidocaldarius
80000
-
gel filtration Sulfolobus acidocaldarius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Sulfolobus acidocaldarius actual substrate is magnesium diphosphate or dimagnesium diphosphate 2 phosphate
-
?
diphosphate + H2O Sulfolobus acidocaldarius 7 actual substrate is magnesium diphosphate or dimagnesium diphosphate 2 phosphate
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol 20% v/v, 82% residual activity Sulfolobus acidocaldarius
isopropanol 20% v/v, 82% residual activity Sulfolobus acidocaldarius
Methanol 20% v/v, 82% residual activity Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius
-
-
-
Sulfolobus acidocaldarius 7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfolobus acidocaldarius

Renatured (Commentary)

Renatured (Comment) Organism
unfolding using guanidine-HCl suggests that the transition between a native tetrameric state and an unfolded state is completely reversible, and essentially independent of any additional factors such as divalent metal cation or dithiothreitol Sulfolobus acidocaldarius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
617
-
56°C Sulfolobus acidocaldarius
617
-
pH 6.5, 56°C Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
Sulfolobus acidocaldarius 2 phosphate
-
?
diphosphate + H2O actual substrate is magnesium diphosphate or dimagnesium diphosphate Sulfolobus acidocaldarius 2 phosphate
-
?
diphosphate + H2O high specificity Sulfolobus acidocaldarius 2 phosphate
-
?
diphosphate + H2O
-
Sulfolobus acidocaldarius 7 2 phosphate
-
?
diphosphate + H2O actual substrate is magnesium diphosphate or dimagnesium diphosphate Sulfolobus acidocaldarius 7 2 phosphate
-
?
diphosphate + H2O high specificity Sulfolobus acidocaldarius 7 2 phosphate
-
?

Subunits

Subunits Comment Organism
More analysis of amino acid composition and comparison with Escherichia coli, saccharomyces cerevisiae and Bacillus sp. enzymes Sulfolobus acidocaldarius
tetramer 4 * 21000, SDS-PAGE Sulfolobus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
Sulfolobus acidocaldarius

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 100
-
Sulfolobus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
10 min, no loss of activity Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Sulfolobus acidocaldarius
6.5
-
56°C Sulfolobus acidocaldarius

pI Value

Organism Comment pI Value Maximum pI Value
Sulfolobus acidocaldarius isoelectric focusing
-
5.1