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Literature summary for 3.6.1.1 extracted from
- Identification and characterization of two isoforms of soluble alkaline inorganic pyrophosphatase from sugar cane leaves (1995), Plant Physiol. Biochem., 33, 39-46.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 10 mM | Saccharum officinarum |  |
| NaF | 1 mM | Saccharum officinarum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.057 | - |
diphosphate | isoform 1 | Saccharum officinarum | |
| 0.072 | - |
diphosphate | isoform 2 | Saccharum officinarum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Saccharum officinarum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Saccharum officinarum | |
| Mg2+ | no other divalent cations can substitute for Mg2+ | Saccharum officinarum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
gel filtration | Saccharum officinarum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | Saccharum officinarum | - |
2 phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharum officinarum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharum officinarum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Saccharum officinarum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.7 | - |
isoform 1 | Saccharum officinarum |
| 6 | - |
isoform 2 | Saccharum officinarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | - |
Saccharum officinarum | 2 phosphate | - |
? | |
| diphosphate + H2O | high specificity | Saccharum officinarum | 2 phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
isoform 2, 6 mM MgCl2, 1mM diphosphate, isoform 1 in presence of 10 mM MgCl2 | Saccharum officinarum |
| 9 | - |
isoform 1, 6 mM MgCl2, 1mM diphosphate | Saccharum officinarum |
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