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Literature summary for 3.6.1.1 extracted from

  • Baykov, A.A.; Hyytiไ, T.; Turkina, M.V.; Efimova, I.S.; Kasho, V.N.; Goldman, A.; Cooperman, B.S.; Lahti, R.
    Functional characterization of Escherichia coli inorganic pyrophosphatase in zwitterionic buffers (1999), Eur. J. Biochem., 260, 308-317.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00045
-
Mg-diphosphate pH 7.2, 1 mM Mg2+, 0.1 M TES/KOH Escherichia coli
0.00053
-
Mg-diphosphate pH 7.2, 1 mM Mg2+, 0.1 M MOPS/KOH Escherichia coli
0.00116
-
Mg-diphosphate pH 7.2, 1 mM Mg2+, 0.08 M monoethalamine/HCl + 0.02 M Tes/KOH Escherichia coli
0.00133
-
Mg-diphosphate pH 7.2, 1 mM Mg2+, 0.1 M Tris-HCl + 0.05 M KCl Escherichia coli
0.00144
-
Mg-diphosphate pH 7.2, 1 mM Mg2+, 0.08 M Tes/Tris + 0.02 M Tes/KOH Escherichia coli
0.00168
-
Mg-diphosphate pH 7.2, 1 mM Mg2+, 0.2 M Tes/Tris Escherichia coli
0.00196
-
Mg-diphosphate pH 7.2, 1 mM Mg2+, 0.15 M Tris-HCl or 0.08 M 2-amino-2-methyl-1,3-propanediol/HCl + 0.02 M Tes/KOH Escherichia coli
0.0055
-
Mg-diphosphate pH 7.2, 1 mM Mg2+, 0.08 M NH4Cl + 0.02 M TES/KOH Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity and stability Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
6 * 20000, sedimentation equilibrium Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Escherichia coli actual substrate is magnesium diphosphate or dimagnesium diphosphate 2 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O actual substrate is magnesium diphosphate or dimagnesium diphosphate Escherichia coli 2 phosphate
-
?
diphosphate + H2O diphosphate in form of Mg-diphosphate Escherichia coli 2 phosphate
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 20000, sedimentation equilibrium Escherichia coli