Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Guanidine HCl | - |
Acidithiobacillus thiooxidans | |
iodoacetamide | - |
Acidithiobacillus thiooxidans | |
KCN | - |
Acidithiobacillus thiooxidans | |
N-ethylmaleimide | - |
Acidithiobacillus thiooxidans | |
NaF | - |
Acidithiobacillus thiooxidans | |
NaN3 | - |
Acidithiobacillus thiooxidans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Acidithiobacillus thiooxidans | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 25% relative activity compared to Mg2+ | Acidithiobacillus thiooxidans | |
Mg2+ | maximum activity | Acidithiobacillus thiooxidans | |
Mn2+ | 10.6% relative activity compared to Mg2+ | Acidithiobacillus thiooxidans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
4 * 20000, SDS-PAGE | Acidithiobacillus thiooxidans |
88000 | - |
SDS-PAGE | Acidithiobacillus thiooxidans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Acidithiobacillus thiooxidans | - |
2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus thiooxidans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acidithiobacillus thiooxidans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
804.1 | - |
- |
Acidithiobacillus thiooxidans |
Storage Stability | Organism |
---|---|
0°C, purified enzyme, more than 9 months | Acidithiobacillus thiooxidans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | 2.6% relative activity compared to diphosphate | Acidithiobacillus thiooxidans | ? | - |
? | |
ATP + H2O | 4.2% relative activity compared to diphosphate | Acidithiobacillus thiooxidans | ? | - |
? | |
diphosphate + H2O | - |
Acidithiobacillus thiooxidans | 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 20000, SDS-PAGE | Acidithiobacillus thiooxidans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
10 min | Acidithiobacillus thiooxidans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Acidithiobacillus thiooxidans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.9 | 9.5 | 4.5% and 72.2% relative activity at pH 5.9 and 9.5 respectively | Acidithiobacillus thiooxidans |