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Literature summary for 3.6.1.1 extracted from

  • Schwarm, H.M.; Vigenschow, H.; Knobloch, K.
    Purification and properties of a soluble inorganic pyrophosphatase from Rhodopseudomonas palustris (1986), Biol. Chem. Hoppe-Seyler, 367, 119-126.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole
-
Rhodopseudomonas palustris
Ba2+
-
Rhodopseudomonas palustris
EDTA
-
Rhodopseudomonas palustris
F-
-
Rhodopseudomonas palustris
free divalent cations
-
Rhodopseudomonas palustris
I2
-
Rhodopseudomonas palustris
Imidodiphosphate
-
Rhodopseudomonas palustris
iodoacetate
-
Rhodopseudomonas palustris
phenylmercuric acetate
-
Rhodopseudomonas palustris
Zn2+
-
Rhodopseudomonas palustris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
Ca-diphosphate
-
Rhodopseudomonas palustris
0.23
-
Mg-diphosphate
-
Rhodopseudomonas palustris
0.29
-
Co-diphosphate
-
Rhodopseudomonas palustris
0.37
-
Mn-diphosphate
-
Rhodopseudomonas palustris

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ most potent activator Rhodopseudomonas palustris
Co2+ activating Rhodopseudomonas palustris
Mg2+
-
Rhodopseudomonas palustris
Mn2+ activating Rhodopseudomonas palustris
Zn2+ activating Rhodopseudomonas palustris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
2 * 32000, SDS-PAGE Rhodopseudomonas palustris
64500
-
-
Rhodopseudomonas palustris
65500
-
gel filtration Rhodopseudomonas palustris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Rhodopseudomonas palustris actual substrate is magnesium diphosphate or dimagnesium diphosphate 2 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Rhodopseudomonas palustris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodopseudomonas palustris

Storage Stability

Storage Stability Organism
-20°C, for several months Rhodopseudomonas palustris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O actual substrate is magnesium diphosphate or dimagnesium diphosphate Rhodopseudomonas palustris 2 phosphate
-
?
diphosphate + H2O diphosphate in form of Mg-diphosphate Rhodopseudomonas palustris 2 phosphate
-
?
diphosphate + H2O diphosphate in form of Ca-diphosphate Rhodopseudomonas palustris 2 phosphate
-
?
diphosphate + H2O diphosphate in form of Co-diphosphate Rhodopseudomonas palustris 2 phosphate
-
?
diphosphate + H2O diphosphate in form of Mn-diphosphate Rhodopseudomonas palustris 2 phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE Rhodopseudomonas palustris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
rapid inactivation Rhodopseudomonas palustris