Catabolic pathway for 2-nitroimidazole involves a novel nitrohydrolase that also confers drug resistance
Qu, Y.; Spain, J.C.; Environ. Microbiol. 13, 1010-1017 (2011) 
Data extracted from this reference:
Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli
Mycobacterium sp.
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Mycobacterium sp.
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
NaCl
1 mM, stimulation
Mycobacterium sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-nitroimidazole + H2O
Mycobacterium sp.
-
imidazol-2-one + nitrite
-
-
?
2-nitroimidazole + H2O
Mycobacterium sp. JS330
-
imidazol-2-one + nitrite
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Mycobacterium sp.
F4ZCI3
-
-
Mycobacterium sp. JS330
F4ZCI3
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.011
-
substrate benznidazole, pH 7.2, 24°C
Mycobacterium sp.
0.0236
-
substrate 2-nitroimidazole, pH 7.2, 24°C
Mycobacterium sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-nitroimidazole + H2O
-
717544
Mycobacterium sp.
imidazol-2-one + nitrite
-
-
-
?
2-nitroimidazole + H2O
-
717544
Mycobacterium sp. JS330
imidazol-2-one + nitrite
-
-
-
?
benznidazole + H2O
-
717544
Mycobacterium sp.
N-benzyl-2-(2-oxo-2,3-dihydro-1H-imidazol-1-yl)acetamide + nitrite
-
-
-
?
benznidazole + H2O
-
717544
Mycobacterium sp. JS330
N-benzyl-2-(2-oxo-2,3-dihydro-1H-imidazol-1-yl)acetamide + nitrite
-
-
-
?
additional information
no activity with 4-nitroimidazole, metronidazole, 2,4-dinitroimidazole, 3-nitro-1,2,4-triazol-5-one, nitazoxanide, or tizoxanide
717544
Mycobacterium sp.
?
-
-
-
?
additional information
no activity with 4-nitroimidazole, metronidazole, 2,4-dinitroimidazole, 3-nitro-1,2,4-triazol-5-one, nitazoxanide, or tizoxanide
717544
Mycobacterium sp. JS330
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
2NI denitrase
-
Mycobacterium sp.
2NI nitrohydrolase
-
Mycobacterium sp.
NnhA
-
Mycobacterium sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
24
-
-
Mycobacterium sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
-
Mycobacterium sp.
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactors required, no oxygen consumption
Mycobacterium sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Mycobacterium sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactors required, no oxygen consumption
Mycobacterium sp.
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Mycobacterium sp.
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
NaCl
1 mM, stimulation
Mycobacterium sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-nitroimidazole + H2O
Mycobacterium sp.
-
imidazol-2-one + nitrite
-
-
?
2-nitroimidazole + H2O
Mycobacterium sp. JS330
-
imidazol-2-one + nitrite
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.011
-
substrate benznidazole, pH 7.2, 24°C
Mycobacterium sp.
0.0236
-
substrate 2-nitroimidazole, pH 7.2, 24°C
Mycobacterium sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-nitroimidazole + H2O
-
717544
Mycobacterium sp.
imidazol-2-one + nitrite
-
-
-
?
2-nitroimidazole + H2O
-
717544
Mycobacterium sp. JS330
imidazol-2-one + nitrite
-
-
-
?
benznidazole + H2O
-
717544
Mycobacterium sp.
N-benzyl-2-(2-oxo-2,3-dihydro-1H-imidazol-1-yl)acetamide + nitrite
-
-
-
?
benznidazole + H2O
-
717544
Mycobacterium sp. JS330
N-benzyl-2-(2-oxo-2,3-dihydro-1H-imidazol-1-yl)acetamide + nitrite
-
-
-
?
additional information
no activity with 4-nitroimidazole, metronidazole, 2,4-dinitroimidazole, 3-nitro-1,2,4-triazol-5-one, nitazoxanide, or tizoxanide
717544
Mycobacterium sp.
?
-
-
-
?
additional information
no activity with 4-nitroimidazole, metronidazole, 2,4-dinitroimidazole, 3-nitro-1,2,4-triazol-5-one, nitazoxanide, or tizoxanide
717544
Mycobacterium sp. JS330
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
24
-
-
Mycobacterium sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
-
Mycobacterium sp.
General Information
General Information
Commentary
Organism
metabolism
Initial step in the biodegradation of 2-nitroimidazole by Mycobacterium sp. JS330. This is the first hydrolytic enzyme involved in elimination of the nitro group. The organism grows on 2-nitroimidazole as sole carbon source.
Mycobacterium sp.
General Information (protein specific)
General Information
Commentary
Organism
metabolism
Initial step in the biodegradation of 2-nitroimidazole by Mycobacterium sp. JS330. This is the first hydrolytic enzyme involved in elimination of the nitro group. The organism grows on 2-nitroimidazole as sole carbon source.
Mycobacterium sp.
Other publictions for EC 3.5.99.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
717544
Qu
Catabolic pathway for 2-nitroi ...
Mycobacterium sp., Mycobacterium sp. JS330
Environ. Microbiol.
13
1010-1017
2011
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