Application | Comment | Organism |
---|---|---|
agriculture | development of tomato plants with delayed fruit ripening by Agrobacterium tumefaciens mediated transfer of a gene encoding 1-aminocyclopropane-1-carboxylic acid deaminase, the enzyme delays fruit ripening by decreasing ethylene synthesis | Pseudomonas chlororaphis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and in delayed ripening tomato | Pseudomonas chlororaphis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36500 | - |
x * 36500, enzyme expressed in Escherichia coli, SDS-PAGE | Pseudomonas chlororaphis |
36800 | - |
x * 36800, calculation from amino acid composition | Pseudomonas chlororaphis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas chlororaphis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no modification | the enzyme expressed in Escherichia coli and in delayed ripening tomatoes is not glycosylated | Pseudomonas chlororaphis |
Purification (Comment) | Organism |
---|---|
expressed in delayed ripening tomato plants | Pseudomonas chlororaphis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.36 | - |
expressed in delayed ripening tomatoes, 37°C | Pseudomonas chlororaphis |
Storage Stability | Organism |
---|---|
-80°C, pH 7.5, 8 mg/ml protein, stable for at least 6 months | Pseudomonas chlororaphis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-aminocyclopropane-1-carboxylate + H2O | - |
Pseudomonas chlororaphis | 2-oxobutanoate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36500, enzyme expressed in Escherichia coli, SDS-PAGE | Pseudomonas chlororaphis |
? | x * 36800, calculation from amino acid composition | Pseudomonas chlororaphis |