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Literature summary for 3.5.99.7 extracted from

  • Finn, R.F.; Leimgruber, R.M.; Boyle, D.M.; Jennings, M.G.; Kimack, N.M.; Smith, C.E.; Bishop, B.F.; Frazier, R.B.; Magin, K.M.; Fuchs, R.L.; Reed, A.J.
    Purification and biochemical comparison of 1-aminocyclopropane-1-carboxylic acid deaminase proteins expressed in delayed ripening tomato and Escherichia coli: studies for a food safety assessment (1996), J. Agric. Food Chem., 44, 381-387.
    View publication on PubMed

Application

Application Comment Organism
agriculture development of tomato plants with delayed fruit ripening by Agrobacterium tumefaciens mediated transfer of a gene encoding 1-aminocyclopropane-1-carboxylic acid deaminase, the enzyme delays fruit ripening by decreasing ethylene synthesis Pseudomonas chlororaphis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and in delayed ripening tomato Pseudomonas chlororaphis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36500
-
x * 36500, enzyme expressed in Escherichia coli, SDS-PAGE Pseudomonas chlororaphis
36800
-
x * 36800, calculation from amino acid composition Pseudomonas chlororaphis

Organism

Organism UniProt Comment Textmining
Pseudomonas chlororaphis
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification the enzyme expressed in Escherichia coli and in delayed ripening tomatoes is not glycosylated Pseudomonas chlororaphis

Purification (Commentary)

Purification (Comment) Organism
expressed in delayed ripening tomato plants Pseudomonas chlororaphis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.36
-
expressed in delayed ripening tomatoes, 37°C Pseudomonas chlororaphis

Storage Stability

Storage Stability Organism
-80°C, pH 7.5, 8 mg/ml protein, stable for at least 6 months Pseudomonas chlororaphis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-aminocyclopropane-1-carboxylate + H2O
-
Pseudomonas chlororaphis 2-oxobutanoate + NH3
-
?

Subunits

Subunits Comment Organism
? x * 36500, enzyme expressed in Escherichia coli, SDS-PAGE Pseudomonas chlororaphis
? x * 36800, calculation from amino acid composition Pseudomonas chlororaphis