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Literature summary for 3.5.99.6 extracted from
- Identification, expression and variation of the GNPDA2 gene, and its association with body weight and fatness traits in chicken (2016), PeerJ, 4, e2129 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene GNPDA2, isozyme cGNPDA2-a, DNA and amino acid sequence determination and analysis, four chicken GNPDA2 transcripts, i.e. cGNPDA2-a, cGNPDA2-b, cGNPDA2-c, and cGNPDA2-d, are identified, genetic structure, sequence comparisons and phylogenetic analysis and tree, recombinant overexpression in chicken preadipocytes | Gallus gallus |
| gene GNPDA2, isozyme cGNPDA2-b, DNA and amino acid sequence determination and analysis, four chicken GNPDA2 transcripts, i.e. cGNPDA2-a, cGNPDA2-b, cGNPDA2-c, and cGNPDA2-d, are identified, genetic structure, sequence comparisons and phylogenetic analysis and tree, recombinant overexpression in chicken preadipocytes | Gallus gallus |
| gene GNPDA2, isozyme cGNPDA2-c, DNA and amino acid sequence determination and analysis, four chicken GNPDA2 transcripts, i.e. cGNPDA2-a, cGNPDA2-b, cGNPDA2-c, and cGNPDA2-d, are identified, genetic structure, sequence comparisons and phylogenetic analysis and tree, recombinant overexpression in chicken preadipocytes | Gallus gallus |
| gene GNPDA2, isozyme cGNPDA2-d, DNA and amino acid sequence determination and analysis, four chicken GNPDA2 transcripts, i.e. cGNPDA2-a, cGNPDA2-b, cGNPDA2-c, and cGNPDA2-d, are identified, genetic structure, sequence comparisons and phylogenetic analysis and tree, recombinant overexpression in chicken preadipocytes | Gallus gallus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | enzyme GNPDA2 overexpression and small interfering RNA (siRNA) knockout in chicken preadipocytes, phenotypes, overview. Two single-nucleotide polymorphisms of the GNPDA2 gene are significantly associated with body weight and a number of fatness traits in chicken. Two SNPs, g.6667C > T and g.7393C > T, in 3'-UTR are significantly associated with a number of productive traits in chicken. The genotyped results of these two SNPs are also confirmed by PCR-RFLP. The g.6667C > T and g.7393C > T (rs14486239) are both significantly associated with body weight during early stage (1-28 days old) of growth. The g.6667C > T is also significantly associated with chest depth and abdominal fat pad weight. The g.7393C > T is significantly associated with small intestine length, leg muscle weight, dressed weight, eviscerated weight, and semi-eviscerated weight | Gallus gallus |
| additional information | enzyme GNPDA2 overexpression and small interfering RNA (siRNA) knockout in chicken preadipocytes, phenotypes, overview. Two single-nucleotide polymorphisms of the GNPDA2 gene are significantly associated with body weight and a number of fatness traits in chicken. Two SNPs, g.6667C > T and g.7393C > T, in 3'-UTR are significantly associated with a number of productive traits in chicken. The genotypes resulting of these two SNPs are also confirmed by PCR-RFLP. The g.6667C > T and g.7393C > T (rs14486239) are both significantly associated with body weight during early stage (1-28 days old) of growth. The g.6667C > T is also significantly associated with chest depth and abdominal fat pad weight. The g.7393C > T is significantly associated with small intestine length, leg muscle weight, dressed weight, eviscerated weight, and semi-eviscerated weight | Gallus gallus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | allosterically regulated enzyme | Gallus gallus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucosamine 6-phosphate + H2O | Gallus gallus | - |
D-fructose 6-phosphate + NH3 | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | E1C878 | cGNPDA2-a; Xinghua | - |
| Gallus gallus | I7C3V5 | cGNPDA2-b; Xinghua | - |
| Gallus gallus | U5LV87 | cGNPDA2-c; Xinghua | - |
| Gallus gallus | U5LXR4 | cGNPDA2-d; Xinghua | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipose tissue | subcutaneous fat and abdominal fat, high expression level | Gallus gallus | - |
| cerebellum | low expression level | Gallus gallus | - |
| duodenum | high expression level | Gallus gallus | - |
| gizzard | low expression level | Gallus gallus | - |
| glandular stomach | low expression level | Gallus gallus | - |
| hypophysis | - |
Gallus gallus | - |
| hypothalamus | high expression level | Gallus gallus | - |
| kidney | low expression level | Gallus gallus | - |
| liver | - |
Gallus gallus | - |
| lung | - |
Gallus gallus | - |
| additional information | analysis of effects on the enzyme by fasting and high-glucose-fat diet treatments, association analysis of variations of GNPDA2 with productive traits in chicken tissues, overview. The complete transcript GNPDA2-a is predominantly expressed in adipose tissue (subcutaneous and abdominal fat), hypothalamus, and duodenum. In fasting chickens, the mRNA level of GNPDA2 is decreased by 58.8% in hypothalamus, and returned to normal level after refeeding. High-glucose-fat diet fed chicken show GNPDA2 gene expression about 2fold higher in adipose tissue compared to control (basal diet), but decreased expression in hypothalamus. Two single-nucleotide polymorphisms of the GNPDA2 gene are significantly associated with body weight and a number of fatness traits in chicken. Tissue specific expression of GNPDA2 variants in Xinghua chicken, no significantly different in expression level of all tissues between female and male chicken | Gallus gallus | - |
| additional information | analysis of effects on the enzyme by fasting and high-glucose-fat diet treatments, association analysis of variations of GNPDA2 with productive traits in chicken tissues, overview. The complete transcript GNPDA2-a is predominantly expressed in adipose tissue (subcutaneous and abdominal fat), hypothalamus, and duodenum. In fasting chickens, the mRNA level of GNPDA2 is decreased by 58.8% in hypothalamus, and returned to normal level after refeeding. High-glucose-fat diet fed chicken show GNPDA2 gene expression about 2fold higher in adipose tissue compared to control (basal diet), but decreased expression in hypothalamus. Two single-nucleotide polymorphisms of the GNPDA2 gene are significantly associated with body weight and a number of fatness traits in chicken. Tissue specific expression of GNPDA2 variants in Xinghua chicken, no significantly different in expression level of all tissues between female and male chickens | Gallus gallus | - |
| pectoral muscle | low expression level | Gallus gallus | - |
| preadipocyte | - |
Gallus gallus | - |
| spleen | - |
Gallus gallus | - |
| telencephalon | low expression level | Gallus gallus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucosamine 6-phosphate + H2O | - |
Gallus gallus | D-fructose 6-phosphate + NH3 | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cGNPDA2-a | - |
Gallus gallus |
| cGNPDA2-b | - |
Gallus gallus |
| cGNPDA2-c | - |
Gallus gallus |
| cGNPDA2-d | - |
Gallus gallus |
| glucosamine-6-phosphate deaminase 2 | - |
Gallus gallus |
| glucosamine-6-phosphate deaminase 2 isoform 2 | - |
Gallus gallus |
| glucosamine-6-phosphate deaminase 2 isoform 3 | - |
Gallus gallus |
| glucosamine-6-phosphate isomerase | UniProt | Gallus gallus |
| GNPDA2 | - |
Gallus gallus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Gallus gallus | the level of cGNPDA2 mRNA is regulated by body weight, feeding/fasting and by consumption of the high-glucose-fat diet, overview | additional information |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the GNPDA2 (glucosamine-6-phosphate deaminase 2) gene is a member of glucosamine-6-phosphate (GlcN6P) deaminase subfamily | Gallus gallus |
| malfunction | two single-nucleotide polymorphisms of the GNPDA2 gene are significantly associated with body weight and a number of fatness traits in chicken, overview | Gallus gallus |
| physiological function | the GNPDA2 gene encodes an allosteric enzyme of glucosamine 6-phosphate deaminase (GlcN6P), which can catalyzes the reversible conversion of D-glucosamine 6-phosphate into D-fructose-6-phosphate and ammonium. The GNPDA2 gene has a potential role in the regulation of body weight, fat and energy metabolism in chicken | Gallus gallus |
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