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Literature summary for 3.5.99.6 extracted from

  • Alvarez-Anorve, L.I.; Alonzo, D.A.; Mora-Lugo, R.; Lara-Gonzalez, S.; Bustos-Jaimes, I.; Plumbridge, J.; Calcagno, M.L.
    Allosteric kinetics of the isoform 1 of human glucosamine-6-phosphate deaminase (2011), Biochim. Biophys. Acta, 1814, 1846-1853.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
N-acetylglucosamine 6-phosphate GlcNAc6P, allosteric site ligand, behaving as a V activator and a K inhibitor: in the absence of GlcNAc6P, the apparent kcat of the enzyme is so low, that GlcNAc6P behaves as an essential activator. Additionally, substrate inhibition, dependent on GlcNAc6P concentration, is observed, Monod allosteric model with some additional postulates, overview. The ligand binds to either enzyme conformational state, but with higher affinity for the R form Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of isozyme GNPDA1 in Escherichia coli strain IBPC 590 (DELTAnagEBACD DELTAlac), which carries a deletion of the chromosomal copy of the nag operon and expresses the recombinant enzyme constitutively from the lac promoter due to the lacI mutation Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
isozyme GNPDA1, X-ray diffraction structure determination and analysis at 1.75 A resolution Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information generation of mutants truncated at positions 260 and 275 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information allosteric enzyme, hGNPDA1 enzyme displays hyperbolic kinetics but very low-activity in the absence of GlcNAc6P, kinetic analysis and modelling of both reaction directions, detailed overview Homo sapiens
0.27
-
D-glucosamine 6-phosphate recombinant wild-type isozyme GNPDA1, pH 8.0, 30°C, kinetic method 1, in absence of N-acetylglucosamine 6-phosphate Homo sapiens
2
-
D-glucosamine 6-phosphate recombinant isozyme GNPDA1 275 truncation mutant, pH 8.0, 30°C, kinetic method 1 Homo sapiens
16.5
-
D-glucosamine 6-phosphate recombinant wild-type isozyme GNPDA1, pH 8.0, 30°C, kinetic method 1, in presence of saturating concentration of N-acetylglucosamine 6-phosphate Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucosamine 6-phosphate + H2O Homo sapiens
-
D-fructose 6-phosphate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P46926 isozyme GNPDA1; isozyme GNPDA1
-
Homo sapiens Q8TDQ7 isozyme GNPDA2; isozyme GNPDA2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isozyme GNPDA1 from Escherichia coli strain IBPC 590 by single purification step by means of allosteric-site affinity chromatography on N-6-aminohexanoyl-GlcN6P agarose Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucosamine 6-phosphate + H2O
-
Homo sapiens D-fructose 6-phosphate + NH3
-
?

Synonyms

Synonyms Comment Organism
GNPDA
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
41.2
-
D-glucosamine 6-phosphate recombinant isozyme GNPDA1 268 truncation mutant, pH 8.0, 30°C, kinetic method 2 Homo sapiens
44.2
-
D-glucosamine 6-phosphate recombinant isozyme GNPDA1 275 truncation mutant, pH 8.0, 30°C, kinetic method 1 Homo sapiens
74
-
D-glucosamine 6-phosphate recombinant isozyme GNPDA1 275 truncation mutant, pH 8.0, 30°C, kinetic method 2 Homo sapiens
228
-
D-glucosamine 6-phosphate recombinant wild-type isozyme GNPDA1, pH 8.0, 30°C, kinetic method 1, in presence of saturating concentration of N-acetylglucosamine 6-phosphate Homo sapiens
260
-
D-glucosamine 6-phosphate recombinant wild-type isozyme GNPDA1, pH 8.0, 30°C, kinetic method 2, in presence of saturating concentration of N-acetylglucosamine 6-phosphate Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

General Information

General Information Comment Organism
additional information possible functional significance of the C-terminal extension of hGNPDA1 isozyme, which is not present in isoform 2 Homo sapiens
physiological function hGNPDA1 can be important for the maintenance of an adequate level of the pool of the UDP-GlcNAc6P, the N-acetylglucosylaminyl donor for many reactions in the cell Homo sapiens