Activating Compound | Comment | Organism | Structure |
---|---|---|---|
N-acetylglucosamine 6-phosphate | GlcNAc6P, allosteric site ligand, behaving as a V activator and a K inhibitor: in the absence of GlcNAc6P, the apparent kcat of the enzyme is so low, that GlcNAc6P behaves as an essential activator. Additionally, substrate inhibition, dependent on GlcNAc6P concentration, is observed, Monod allosteric model with some additional postulates, overview. The ligand binds to either enzyme conformational state, but with higher affinity for the R form | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of isozyme GNPDA1 in Escherichia coli strain IBPC 590 (DELTAnagEBACD DELTAlac), which carries a deletion of the chromosomal copy of the nag operon and expresses the recombinant enzyme constitutively from the lac promoter due to the lacI mutation | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
isozyme GNPDA1, X-ray diffraction structure determination and analysis at 1.75 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of mutants truncated at positions 260 and 275 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | allosteric enzyme, hGNPDA1 enzyme displays hyperbolic kinetics but very low-activity in the absence of GlcNAc6P, kinetic analysis and modelling of both reaction directions, detailed overview | Homo sapiens | |
0.27 | - |
D-glucosamine 6-phosphate | recombinant wild-type isozyme GNPDA1, pH 8.0, 30°C, kinetic method 1, in absence of N-acetylglucosamine 6-phosphate | Homo sapiens | |
2 | - |
D-glucosamine 6-phosphate | recombinant isozyme GNPDA1 275 truncation mutant, pH 8.0, 30°C, kinetic method 1 | Homo sapiens | |
16.5 | - |
D-glucosamine 6-phosphate | recombinant wild-type isozyme GNPDA1, pH 8.0, 30°C, kinetic method 1, in presence of saturating concentration of N-acetylglucosamine 6-phosphate | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucosamine 6-phosphate + H2O | Homo sapiens | - |
D-fructose 6-phosphate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P46926 | isozyme GNPDA1; isozyme GNPDA1 | - |
Homo sapiens | Q8TDQ7 | isozyme GNPDA2; isozyme GNPDA2 | - |
Purification (Comment) | Organism |
---|---|
recombinant isozyme GNPDA1 from Escherichia coli strain IBPC 590 by single purification step by means of allosteric-site affinity chromatography on N-6-aminohexanoyl-GlcN6P agarose | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucosamine 6-phosphate + H2O | - |
Homo sapiens | D-fructose 6-phosphate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GNPDA | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
41.2 | - |
D-glucosamine 6-phosphate | recombinant isozyme GNPDA1 268 truncation mutant, pH 8.0, 30°C, kinetic method 2 | Homo sapiens | |
44.2 | - |
D-glucosamine 6-phosphate | recombinant isozyme GNPDA1 275 truncation mutant, pH 8.0, 30°C, kinetic method 1 | Homo sapiens | |
74 | - |
D-glucosamine 6-phosphate | recombinant isozyme GNPDA1 275 truncation mutant, pH 8.0, 30°C, kinetic method 2 | Homo sapiens | |
228 | - |
D-glucosamine 6-phosphate | recombinant wild-type isozyme GNPDA1, pH 8.0, 30°C, kinetic method 1, in presence of saturating concentration of N-acetylglucosamine 6-phosphate | Homo sapiens | |
260 | - |
D-glucosamine 6-phosphate | recombinant wild-type isozyme GNPDA1, pH 8.0, 30°C, kinetic method 2, in presence of saturating concentration of N-acetylglucosamine 6-phosphate | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | possible functional significance of the C-terminal extension of hGNPDA1 isozyme, which is not present in isoform 2 | Homo sapiens |
physiological function | hGNPDA1 can be important for the maintenance of an adequate level of the pool of the UDP-GlcNAc6P, the N-acetylglucosylaminyl donor for many reactions in the cell | Homo sapiens |