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Literature summary for 3.5.99.6 extracted from

  • Kikuchi, K.; Kikuchi, H.
    Stabilization and purification of glucosamine 6-phosphate isomerase from rat kidney (1979), Sci. Rep. Res. Inst. Tohoku Univ. Med., 26, 92-98.
    View publication on PubMed

General Stability

General Stability Organism
D-glucosamine 6-phosphate and N-acetylglucosamine 6-phosphate stabilize against heat inactivation at 60°C, and protect the enzyme from tryptic digestion Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
PCMB protection by 0.3 mM glucosamine 6-phosphate, no protection by 0.3 mM N-acetylglucosamine 6-phosphate Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-70°C, stable for 70 days Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucosamine 6-phosphate + H2O
-
Rattus norvegicus D-fructose 6-phosphate + NH3
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
10 min, in presence of 1.7 mM glucosamine 6-phosphate or N-acetylglucosamine 6-phosphate, 7% loss of activity Rattus norvegicus
60
-
90% loss of activity within 1 min without stabilizer, stable for 1 min in presence of 0.6 mM glucosamine 6-phosphate or N-acetylglucosamine 6-phosphate Rattus norvegicus
65
-
complete loss of activity Rattus norvegicus