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Literature summary for 3.5.99.6 extracted from
- Glucosamine metabolism. IV. Glucosamine-6-phosphate deaminase (1958), J. Biol. Chem., 232, 807-827.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| N-Acetylgalactosamine 6-phosphate | activates | Sus scrofa |  |
| N-acetylglucosamine 6-phosphate | activates | Escherichia coli | |
| N-acetylglucosamine 6-phosphate | activates | Sus scrofa | |
| N-acetylglucosamine 6-phosphate | increases reaction velocity when D-glucosamine 6-phosphate is present at levels below that required for saturation | Escherichia coli | |
General Stability
| General Stability | Organism |
|---|---|
| labile to freezing and thawing | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.66 | - |
D-glucosamine 6-phosphate | pH 7.9, 37°C, activated by N-acetylglucosamine 6-phosphate | Sus scrofa | |
| 1.2 | - |
D-glucosamine 6-phosphate | pH 7.8, 37°C, activated by N-acetylglucosamine 6-phosphate | Escherichia coli | |
| 7.1 | - |
D-glucosamine 6-phosphate | pH 7.8, 37°C | Escherichia coli | |
| 14 | - |
D-glucosamine 6-phosphate | pH 7.8, 37°C, without activator | Sus scrofa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Sus scrofa | - |
hog | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 52 | - |
- |
Sus scrofa |
| 158 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, little loss of activity after 2-3 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucosamine 6-phosphate + H2O | - |
Sus scrofa | D-fructose 6-phosphate + NH3 | - |
r | |
| D-glucosamine 6-phosphate + H2O | specific for | Escherichia coli | D-fructose 6-phosphate + NH3 | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.2 | - |
Sus scrofa |
| 7.5 | - |
enzyme activated by N-acetylgalactosamine 6-phosphate | Sus scrofa |
| 7.8 | - |
- |
Escherichia coli |
| 8.8 | - |
enzyme activated by N-acetylglucosamine 6-phosphate | Sus scrofa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | pH 6.0: about 25% of maximal activity, pH 8.5: about 60% of maximal activity | Escherichia coli |
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