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Literature summary for 3.5.99.2 extracted from

  • Zallot, R.; Yazdani, M.; Goyer, A.; Ziemak, M.J.; Guan, J.C.; McCarty, D.R.; de Crecy-Lagard, V.; Gerdes, S.; Garrett, T.J.; Benach, J.; Hunt, J.F.; Shintani, D.K.; Hanson, A.D.
    Salvage of the thiamin pyrimidine moiety by plant TenA proteins lacking an active-site cysteine (2014), Biochem. J., 463, 145-155.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.99.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-amino-5-aminomethyl-2-methylpyrimidine + H2O Bacillus subtilis
-
 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3
-
 ?
4-amino-5-aminomethyl-2-methylpyrimidine + H2O Bacillus subtilis 168
-
 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3
-
 ?
additional information Bacillus subtilis no activity with N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine, thiamin monophosphate, thiamin diphosphate, thiamin disulfide, desthiothiamin, and thiochrome ?
-
 ?
additional information Bacillus subtilis 168 no activity with N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine, thiamin monophosphate, thiamin diphosphate, thiamin disulfide, desthiothiamin, and thiochrome ?
-
 ?
oxothiamin + H2O Bacillus subtilis
-
 ?
-
 ?
oxothiamin + H2O Bacillus subtilis 168
-
 ?
-
 ?
oxythiamin + H2O Bacillus subtilis
-
 ?
-
 ?
oxythiamin + H2O Bacillus subtilis 168
-
 ?
-
 ?
thiamine + H2O Bacillus subtilis
-
 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?
thiamine + H2O Bacillus subtilis 168
-
 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P25052
-
-
Bacillus subtilis 168 P25052
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ -affinity column chromatography Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.091
-
 with oxothiamin as substrate, at 30°C in 45 mM Tris/HCl buffer, pH 7.5, containing 0.9 mM dithiothreitol and 0.45 M glycinebetaine Bacillus subtilis
0.162
-
 with thiamine as substrate, at 30°C in 45 mM Tris/HCl buffer, pH 7.5, containing 0.9 mM dithiothreitol and 0.45 M glycinebetaine Bacillus subtilis
0.205
-
 with oxythiamin as substrate, at 30°C in 45 mM Tris/HCl buffer, pH 7.5, containing 0.9 mM dithiothreitol and 0.45 M glycinebetaine Bacillus subtilis
0.292
-
 with 4-amino-5-aminomethyl-2-methylpyrimidine as substrate, at 30°C in 45 mM Tris/HCl buffer, pH 7.5, containing 0.9 mM dithiothreitol and 0.45 M glycinebetaine Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-amino-5-aminomethyl-2-methylpyrimidine + H2O
-
 Bacillus subtilis 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3
-
 ?
4-amino-5-aminomethyl-2-methylpyrimidine + H2O
-
 Bacillus subtilis 168 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3
-
 ?
additional information no activity with N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine, thiamin monophosphate, thiamin diphosphate, thiamin disulfide, desthiothiamin, and thiochrome Bacillus subtilis ?
-
 ?
additional information no activity with N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine, thiamin monophosphate, thiamin diphosphate, thiamin disulfide, desthiothiamin, and thiochrome Bacillus subtilis 168 ?
-
 ?
oxothiamin + H2O
-
 Bacillus subtilis ?
-
 ?
oxothiamin + H2O
-
 Bacillus subtilis 168 ?
-
 ?
oxythiamin + H2O
-
 Bacillus subtilis ?
-
 ?
oxythiamin + H2O
-
 Bacillus subtilis 168 ?
-
 ?
thiamine + H2O
-
 Bacillus subtilis 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?
thiamine + H2O
-
 Bacillus subtilis 168 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
TenA_C
-
 Bacillus subtilis
thiaminase
-
 Bacillus subtilis