Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Helicobacter pylori |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion method, using 0.1 m Tris (pH 8.5), 1.1 M lithium sulfate | Helicobacter pylori |
Protein Variants | Comment | Organism |
---|---|---|
F47Y | poorly active mutant. At pH 6.0, its activity is absent. Moreover, the enzyme does not present any activity on thiamin degradation | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.058 | - |
4-amino-5-aminomethyl-2-methylpyrimidine | wild type enzyme, in 30 mM Tris, 50 mM NaCl (pH 8.0), at 20°C | Helicobacter pylori | |
0.068 | - |
4-amino-5-aminomethyl-2-methylpyrimidine | mutant enzyme F47Y, in 30 mM Tris, 50 mM NaCl (pH 8.0), at 20°C | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
4 * 25000, SDS-PAGE | Helicobacter pylori |
25643 | - |
4 * 25643, calculated from amino acid sequence | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | O25874 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography and Superdex 200 gel filtration | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-amino-5-aminomethyl-2-methylpyrimidine + H2O | the wild type enzyme is poorly active on 4-amino-5-aminoethyl-2-methylpyrimidine | Helicobacter pylori | 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 25000, SDS-PAGE | Helicobacter pylori |
homotetramer | 4 * 25643, calculated from amino acid sequence | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
HP1287 | - |
Helicobacter pylori |
TenA | - |
Helicobacter pylori |
thiaminase II | - |
Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
4-amino-5-aminomethyl-2-methylpyrimidine | mutant enzyme F47Y, in 30 mM Tris, 50 mM NaCl (pH 8.0), at 20°C | Helicobacter pylori | |
0.003 | - |
4-amino-5-aminomethyl-2-methylpyrimidine | wild type enzyme, in 30 mM Tris, 50 mM NaCl (pH 8.0), at 20°C | Helicobacter pylori |
General Information | Comment | Organism |
---|---|---|
metabolism | TenA plays a pivotal role in the thiamin biosynthetic route | Helicobacter pylori |