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Literature summary for 3.5.99.2 extracted from

  • Wittliff, J.L.; Airth, R.L.
    Thiaminase II (thiamine hydrolase, EC 3.5.99.2) (1971), Methods Enzymol., 18, 234-238.
No PubMed abstract available
Search for more literature for 3.5.99.2

Activating Compound

Activating Compound Comment Organism Structure
EDTA in a concentration range of 0.02 mM to 1000 mM Aneurinibacillus aneurinilyticus
L-cysteine in a concentration range of 0.02 mM to 1000 mM Aneurinibacillus aneurinilyticus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 0.02 mM Aneurinibacillus aneurinilyticus
Fe2+ 0.02 mM Aneurinibacillus aneurinilyticus
additional information inhibited by many aromatic and heterocyclic amines Aneurinibacillus aneurinilyticus
p-chloromercuribenzoate 0.02 mM Aneurinibacillus aneurinilyticus
Zn2+ 0.02 mM Aneurinibacillus aneurinilyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 thiamine
-
 Aneurinibacillus aneurinilyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 ultracentrifugation Aneurinibacillus aneurinilyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thiamine + H2O Candida sp. (in: Saccharomycetales)
-
 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?
thiamine + H2O Geotrichum candidum
-
 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?
thiamine + H2O Aneurinibacillus aneurinilyticus
-
 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?
thiamine + H2O Trichosporon aneurinolyticum
-
 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?

Organism

Organism UniProt Comment Textmining
Aneurinibacillus aneurinilyticus
-
-
-
Candida sp. (in: Saccharomycetales)
-
-
-
Geotrichum candidum
-
-
-
Trichosporon aneurinolyticum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aneurinibacillus aneurinilyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiamine + H2O
-
 Candida sp. (in: Saccharomycetales) 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?
thiamine + H2O
-
 Geotrichum candidum 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?
thiamine + H2O
-
 Aneurinibacillus aneurinilyticus 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?
thiamine + H2O
-
 Trichosporon aneurinolyticum 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 100000, SDS-PAGE Aneurinibacillus aneurinilyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 40
-
 Candida sp. (in: Saccharomycetales)
40 45
-
 Trichosporon aneurinolyticum
60
-
-
 Geotrichum candidum
60
-
-
 Aneurinibacillus aneurinilyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 enzyme inactivated by heating for 10 min Candida sp. (in: Saccharomycetales)
60
-
 enzyme inactivated by heating for 10 min Trichosporon aneurinolyticum
80
-
 enzyme inactivated by heating for 10 min Geotrichum candidum
80
-
 enzyme inactivated by heating for 10 min Aneurinibacillus aneurinilyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Candida sp. (in: Saccharomycetales)
7
-
-
 Geotrichum candidum
7
-
-
 Trichosporon aneurinolyticum
8.6
-
-
 Aneurinibacillus aneurinilyticus