Cloned (Comment) | Organism |
---|---|
- |
Comamonas testosteroni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 2 mM | Comamonas testosteroni | |
Cu2+ | 2 mM, complete inhibition | Comamonas testosteroni | |
diethyl dicarbonate | 10 mM | Comamonas testosteroni | |
Mg2+ | 2 mM | Comamonas testosteroni | |
Ni2+ | 2 mM, complete inhibition | Comamonas testosteroni | |
p-chloromercuribenzoate | 0.2 mM, complete inhibition | Comamonas testosteroni | |
Zn2+ | 2 mM, complete inhibition | Comamonas testosteroni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
2-aminomuconate | pH and temperature not specified in the publication | Comamonas testosteroni | |
0.0088 | - |
2-amino-5-chloromuconate | pH and temperature not specified in the publication | Comamonas testosteroni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
1 * 28000, SDS-PAGE | Comamonas testosteroni |
30000 | - |
gel filtration | Comamonas testosteroni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminomuconate + H2O | Comamonas testosteroni | the enzyme is involved in chloronitrobenzene and nitrobenzene degradation | (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3 | - |
? | |
2-aminomuconate + H2O | Comamonas testosteroni CNB-1 | the enzyme is involved in chloronitrobenzene and nitrobenzene degradation | (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | - |
- |
- |
Comamonas testosteroni CNB-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Comamonas testosteroni |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.147 | - |
substrate: 2-aminomuconate, pH and temperature not specified in the publication | Comamonas testosteroni |
0.196 | - |
substrate: 2-amino-5-chloromuconate, pH and temperature not specified in the publication | Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-5-chloromuconate + H2O | - |
Comamonas testosteroni | 2-hydroxy-5-chloromuconate + NH3 | - |
? | |
2-amino-5-chloromuconate + H2O | - |
Comamonas testosteroni CNB-1 | 2-hydroxy-5-chloromuconate + NH3 | - |
? | |
2-aminomuconate + H2O | the enzyme is involved in chloronitrobenzene and nitrobenzene degradation | Comamonas testosteroni | (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3 | - |
? | |
2-aminomuconate + H2O | 2-aminomuconate i.e. (2Z,4E)-2-aminohexa-2,4-dienedioate | Comamonas testosteroni | (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3 | (2Z,4E)-2-hydroxyhexa-2,4-dienedioate i.e. (2Z,4E)-2-hydroxymuconate | ? | |
2-aminomuconate + H2O | the enzyme is involved in chloronitrobenzene and nitrobenzene degradation | Comamonas testosteroni CNB-1 | (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3 | - |
? | |
2-aminomuconate + H2O | 2-aminomuconate i.e. (2Z,4E)-2-aminohexa-2,4-dienedioate | Comamonas testosteroni CNB-1 | (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3 | (2Z,4E)-2-hydroxyhexa-2,4-dienedioate i.e. (2Z,4E)-2-hydroxymuconate | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28000, SDS-PAGE | Comamonas testosteroni |
Synonyms | Comment | Organism |
---|---|---|
cnbZ | - |
Comamonas testosteroni |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in chloronitrobenzene and nitrobenzene degradation | Comamonas testosteroni |