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Literature summary for 3.5.99.11 extracted from

  • He, Z.; Spain, J.C.
    A novel 2-aminomuconate deaminase in the nitrobenzene degradation pathway of Pseudomonas pseudoalcaligenes JS45 (1998), J. Bacteriol., 180, 2502-2506.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 2 mM, 20% inhibition Pseudomonas oleovorans
diethyl dicarbonate 2 mM, complete loss of activity Pseudomonas oleovorans
Mn2+ 2 mM, 20% inhibition Pseudomonas oleovorans
additional information not inhibitory: saturated alpha-amino acids, including glycine, alanine, aspartic acid, glutaric acid, and 2-aminoadipic acid, or EDTA at 25 mM Pseudomonas oleovorans
phenylhydrazine 10 mM, 80% inhibition Pseudomonas oleovorans
Zn2+ 2 mM, 70% inhibition Pseudomonas oleovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.067
-
2-aminomuconate pH 6.6, 25°C Pseudomonas oleovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16600
-
6 * 16600, SDS-PAGE Pseudomonas oleovorans
100000
-
gel filtration and PAGE Pseudomonas oleovorans

Organism

Organism UniProt Comment Textmining
Pseudomonas oleovorans P81593
-
-
Pseudomonas oleovorans JS45 P81593
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
37.8
-
pH 6.6, 25°C Pseudomonas oleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminomuconate + H2O the deamination reaction is irreversible under the assay conditions Pseudomonas oleovorans 4-oxalocrotonate + NH3 enzyme converts 2-aminomuconate directly to 4-oxalocrotonate, rather than 2-hydroxymuconate, which suggests that the deamination is carried out via an imine intermediate ir
2-aminomuconate + H2O the deamination reaction is irreversible under the assay conditions Pseudomonas oleovorans JS45 4-oxalocrotonate + NH3 enzyme converts 2-aminomuconate directly to 4-oxalocrotonate, rather than 2-hydroxymuconate, which suggests that the deamination is carried out via an imine intermediate ir
additional information no substrates: 2-aminomuconic semialdehyde, or saturated alpha-amino acids, including glycine, alanine, aspartic acid, glutaric acid, and 2-aminoadipic acid Pseudomonas oleovorans ?
-
?
additional information no substrates: 2-aminomuconic semialdehyde, or saturated alpha-amino acids, including glycine, alanine, aspartic acid, glutaric acid, and 2-aminoadipic acid Pseudomonas oleovorans JS45 ?
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 16600, SDS-PAGE Pseudomonas oleovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
stable at room temperature for 3 days Pseudomonas oleovorans
60
-
5 min, 23% residual activity Pseudomonas oleovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
208
-
2-aminomuconate pH 6.6, 25°C Pseudomonas oleovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
-
Pseudomonas oleovorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.7 8.8 room temperature, stable for at least 3 h Pseudomonas oleovorans