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Literature summary for 3.5.5.7 extracted from

  • Han, C.; Yao, P.; Yuan, J.; Duan, Y.; Feng, J.; Wang, M.; Wu, Q.; Zhu, D.
    Nitrilase-catalyzed hydrolysis of 3-aminopropionitrile at high concentration with a tandem reaction strategy for shifting the reaction to beta-alanine formation (2015), J. Mol. Catal. B, 115, 113-118 .
No PubMed abstract available
Search for more literature for 3.5.5.7

Application

Application Comment Organism
synthesis the enzyme catalyzes the hydrolysis of 3-aminopropionitrile at high substrate concentration up to 3 M, and optimal pH 7.3 and temperature 30°C. However, with the increase of substrate concentration, 3-aminopropanamide is formed, reaching 33% at the substrate concentration of 3 M. A tandem reaction strategy is developed by introducing the aspartate ammonia-lyase-catalyzed amination of fumarate, which utilizes the by-product ammonia as the amino donor in the formation of aspartic acid. Formation of 3-aminopropanamide is significantly inhibited with its amount being reduced from 33% to 3%. The tandem reaction strategy of removing the byproduct ammonia might offer a possibility of producing x02-alanine and L-aspartic acid in one process if the problem in the separation of these two products was solved Bradyrhizobium japonicum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rosetta2(DE3) Bradyrhizobium japonicum

Organism

Organism UniProt Comment Textmining
Bradyrhizobium japonicum
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Bradyrhizobium japonicum USDA110
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-aminopropionitrile + 2 H2O the enzyme catalyzes the hydrolysis of 3-aminopropionitrile at high substrate concentration up to 3 M. With the increase of substrate concentration, 3-aminopropanamide is formed, reaching 33% at the substrate concentration of 3 M Bradyrhizobium japonicum beta-alanine + NH3
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 ?
3-aminopropionitrile + 2 H2O the enzyme catalyzes the hydrolysis of 3-aminopropionitrile at high substrate concentration up to 3 M. With the increase of substrate concentration, 3-aminopropanamide is formed, reaching 33% at the substrate concentration of 3 M Bradyrhizobium japonicum USDA110 beta-alanine + NH3
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 ?

Synonyms

Synonyms Comment Organism
BjNIT3397
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 Bradyrhizobium japonicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
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-
 Bradyrhizobium japonicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
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-
 Bradyrhizobium japonicum

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5.0: about 75% of maximal activity, pH 8.0: about 75% of maximal activity Bradyrhizobium japonicum