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Literature summary for 3.5.5.7 extracted from

  • Chen, Z.; Chen, H.; Ni, Z.; Tian, R.; Zhang, T.; Jia, J.; Yang, S.
    Expression and characterization of a novel nitrilase from hyperthermophilic bacterium Thermotoga maritima MSB8 (2015), J. Microbiol. Biotechnol., 25, 1660-1669 .
    View publication on PubMed
Search for more literature for 3.5.5.7

Application

Application Comment Organism
industry the enzyme is a potential candidate for industrial applications for biosynthesis of carboxylic acid Thermotoga maritima
synthesis the enzyme is a potential candidate for industrial applications for biosynthesis of carboxylic acid Thermotoga maritima

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM, 25% inhibition Thermotoga maritima
acetone 5%, about 60% inhibition Thermotoga maritima
Ba2+ 1 mM, 18% inhibition Thermotoga maritima
chloroform 5%, about 90% inhibition Thermotoga maritima
Co2+ 1 mM, 21% inhibition Thermotoga maritima
Cu2+ 1 mM, 85% inhibition Thermotoga maritima
DMSO 5%, about 45% inhibition Thermotoga maritima
EDTA 1 mM, 17% inhibition Thermotoga maritima
ethanol 5%, about 10% inhibition Thermotoga maritima
ether 5%, about 70% inhibition Thermotoga maritima
ethyl acetate 5%, about 85% inhibition Thermotoga maritima
Fe3+ 1 mM, 8% inhibition Thermotoga maritima
glycerol 5%, about 40% inhibition Thermotoga maritima
Isopropanol 5%, about 30% inhibition Thermotoga maritima
L-cysteine 1 mM, 6% inhibition Thermotoga maritima
L-glutathione 1 mM, 17% inhibition Thermotoga maritima
methanol 5%, about 30% inhibition Thermotoga maritima
Mn2+ 1 mM, 65% inhibition Thermotoga maritima
Ni2+ 1 mM, 60% inhibition Thermotoga maritima
PMSF 1 mM, 51% inhibition Thermotoga maritima
SDS 1 mM, 63% inhibition Thermotoga maritima
Triton X-100 1 mM, 65% inhibition Thermotoga maritima
Tween-20 1 mM, 54% inhibition Thermotoga maritima
Tween-80 1 mM, 57% inhibition Thermotoga maritima
Zn2+ 1 mM, 54% inhibition Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.63
-
 3-Cyanopyridine pH 7.5, temperature not specified in the publication Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 1 mM, 1.2fold activation Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30070
-
 SDS-PAGE Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9WYX6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-cyanopyridine + 2 H2O
-
 Thermotoga maritima pyridine 3-carboxylic acid + NH3
-
 ?
4-aminobenzonitrile + 2 H2O 57.6% compared to the activity with 3-cyanopyridine Thermotoga maritima 4-aminobenzoic acid + NH3
-
 ?
4-chlorobutyronitrile + 2 H2O 66.7% compared to the activity with 3-cyanopyridine Thermotoga maritima 4-chlorobutanoic acid + NH3
-
 ?
acetonitrile + 2 H2O 19.1% compared to the activity with 3-cyanopyridine Thermotoga maritima acetic acid + NH3
-
 ?
acrylonitrile + 2 H2O 2.8% compared to the activity with 3-cyanopyridine Thermotoga maritima acrylic acid + NH3
-
 ?
butyronitrile + 2 H2O 48.5% compared to the activity with 3-cyanopyridine Thermotoga maritima butyric acid + NH3
-
 ?
dimethylaminopropionitrile + 2 H2O 50.5% compared to the activity with 3-cyanopyridine Thermotoga maritima dimethylaminopropionic acid + NH3
-
 ?
hexanedinitrile + 2 H2O 111% compared to the activity with 3-cyanopyridine Thermotoga maritima ?
-
 ?
malononitrile + 2 H2O 120.5% compared to the activity with 3-cyanopyridine Thermotoga maritima malonic acid + NH3
-
 ?
additional information no activity with mandelonitrile, glycolonitrile Thermotoga maritima ?
-
-
pentanenitrile + 2 H2O 56.3% compared to the activity with 3-cyanopyridine Thermotoga maritima pentanoic acid + NH3
-
 ?
phenylacetonitrile + 2 H2O 16.3% compared to the activity with 3-cyanopyridine Thermotoga maritima phenylacetic acid + NH3
-
 ?
propionitrile + 2 H2O 33.2% compared to the activity with 3-cyanopyridine Thermotoga maritima propionic acid + NH3
-
 ?
succinonitrile + 2 H2O 137.7% compared to the activity with 3-cyanopyridine Thermotoga maritima succinic acid + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 30070, SDS-PAGE Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 50 40°C: about 50% of maximal activity, 50°C: about 40% of maximal activity Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
 60 min, 60% loss of activity Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.44
-
 Succinonitrile pH 7.5, temperature not specified in the publication Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 40% of maximal activity, pH 9.0: about 35% of maximal activity Thermotoga maritima